Intracellular pH in rat pancreatic ducts.

I Novak; M Hug; R Greger

Intracellular pH in rat pancreatic ducts.

I Novak, M Hug, R Greger

Molecular Integrative Physiology

6 Citations (Scopus)

Abstract

In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.

Original language	English
Journal	Comparative biochemistry and physiology. Part A, Physiology
Volume	118
Issue number	2
Pages (from-to)	409-11
Number of pages	2
ISSN	1096-4940
Publication status	Published - 1997

Cite this

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title = "Intracellular pH in rat pancreatic ducts.",

abstract = "In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.",

author = "I Novak and M Hug and R Greger",

note = "Keywords: Animals; Bicarbonates; Chlorides; Hydrogen-Ion Concentration; Pancreatic Ducts; Rats; Rats, Wistar; Sodium-Hydrogen Antiporter",

year = "1997",

language = "English",

volume = "118",

pages = "409--11",

journal = "Comparative Biochemistry and Physiology. Part A: Physiology",

issn = "1096-4940",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Intracellular pH in rat pancreatic ducts.

AU - Novak, I

AU - Hug, M

AU - Greger, R

N1 - Keywords: Animals; Bicarbonates; Chlorides; Hydrogen-Ion Concentration; Pancreatic Ducts; Rats; Rats, Wistar; Sodium-Hydrogen Antiporter

PY - 1997

Y1 - 1997

N2 - In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.

AB - In order to study the mechanism of H+ and HCO3- transport in a HCO3- secreting epithelium, pancreatic ducts, we have measured the intracellular pH (pHi) in this tissue using the pH sensitive probe BCECF. We found that exposures of ducts to solutions containing acetate/acetic acid or NH4+/NH3 buffers (20 mmol/l) led to pHi changes in accordance with entry of lipid-soluble forms of the buffers, followed by back-regulation of pHi by duct cells. In another type of experiment, changes in extracellular pH of solutions containing HEPES or HCO3-/CO2 buffers led to significant changes in pHi that did not seem to be back-regulated efficiently by duct cells. The sensitivity of pHi to the inhibitor HOE 694 and to changes in Na+ gradients, indicate that the Na+/H+ exchanger is present in this epithelium. Similarly, the sensitivity to Cl- and HCO3- gradients indicated the presence of the Cl-/HCO3- exchanger. Under some conditions, these exchangers can be invoked to regulate cell pH.

M3 - Journal article

C2 - 9366082

SN - 1096-4940

VL - 118

SP - 409

EP - 411

JO - Comparative Biochemistry and Physiology. Part A: Physiology

JF - Comparative Biochemistry and Physiology. Part A: Physiology

IS - 2

ER -

Intracellular pH in rat pancreatic ducts.

Abstract

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