TY - JOUR
T1 - Interfacing capillary electrophoresis with inductively coupled plasma mass spectrometry by direct injection nebulization for selenium speciation
AU - Bendahl, Lars
AU - Gammelgaard, Bente
AU - Jons, O.
AU - Farver, O.
AU - Hansen, S.H.
PY - 2001
Y1 - 2001
N2 - A demountable direct injection high efficiency nebulizer operating at low sample uptake rates was developed and used for coupling of capillary electrophoresis (CE) with inductively coupled plasma mass spectrometry (ICP-MS). When the nebulizer was used for continuous sample introduction, detection limits of 20 and 1 ng L-1 were obtained for Se-82 and Rh-103, respectively, at sample uptake rates of 10-30 muL min(-1), based on three times the standard deviation of blank solution (3 sigma (b), n = 10). The nebulizer was used as part of the interface for coupling of CE with ICP-MS and applied for speciation of aqueous selenium standards. The interface was operated in the self-aspirating mode with a sheath liquid uptake of 10 muL min(-1). The CE-ICP-MS system resulted in baseline separation of selenate, selenite, selenocystine and selenomethionine within a total analysis time of 5.4 min. Detection limits were in the sub mug Se L-1 range, corresponding to absolute detection limits in the range 25-125 fg selenium. Repeatability (n = 6) expressed as relative standard deviations with respect to migration times, peak heights and peak areas were better than 1.6, 6.7 and 6.0%, respectively
AB - A demountable direct injection high efficiency nebulizer operating at low sample uptake rates was developed and used for coupling of capillary electrophoresis (CE) with inductively coupled plasma mass spectrometry (ICP-MS). When the nebulizer was used for continuous sample introduction, detection limits of 20 and 1 ng L-1 were obtained for Se-82 and Rh-103, respectively, at sample uptake rates of 10-30 muL min(-1), based on three times the standard deviation of blank solution (3 sigma (b), n = 10). The nebulizer was used as part of the interface for coupling of CE with ICP-MS and applied for speciation of aqueous selenium standards. The interface was operated in the self-aspirating mode with a sheath liquid uptake of 10 muL min(-1). The CE-ICP-MS system resulted in baseline separation of selenate, selenite, selenocystine and selenomethionine within a total analysis time of 5.4 min. Detection limits were in the sub mug Se L-1 range, corresponding to absolute detection limits in the range 25-125 fg selenium. Repeatability (n = 6) expressed as relative standard deviations with respect to migration times, peak heights and peak areas were better than 1.6, 6.7 and 6.0%, respectively
M3 - Journal article
SN - 0267-9477
VL - 16
SP - 38
EP - 42
JO - Journal of Analytical Atomic Spectrometry
JF - Journal of Analytical Atomic Spectrometry
IS - 1
ER -