Interaction of extremophilic archaeal viruses with human and mouse complement system and viral biodistribution in mice

Linping Wu; Kristine Buch Uldahl; Fangfang Chen; Halli Benasutti; Deborah Logvinski; Vivian Vu; Nirmal K. Banda; Xu Peng; Dmitri Simberg; Seyed Moein Moghimi

doi:10.1016/j.molimm.2017.08.009

Interaction of extremophilic archaeal viruses with human and mouse complement system and viral biodistribution in mice

Linping Wu, Kristine Buch Uldahl, Fangfang Chen, Halli Benasutti, Deborah Logvinski, Vivian Vu, Nirmal K. Banda, Xu Peng, Dmitri Simberg, Seyed Moein Moghimi^*

^*Corresponding author for this work

Functional Genomics

3 Citations (Scopus)

Abstract

Archaeal viruses offer exceptional biophysical properties for modification and exploration of their potential in bionanotechnology, bioengineering and nanotherapeutic developments. However, the interaction of archaeal viruses with elements of the innate immune system has not been explored, which is a necessary prerequisite if their potential for biomedical applications to be realized. Here we show complement activation through lectin (via direct binding of MBL/MASPs) and alternative pathways by two extremophilic archaeal viruses (Sulfolobus monocaudavirus 1 and Sulfolobus spindle-shaped virus 2) in human serum. We further show some differences in initiation of complement activation pathways between these viruses. Since, Sulfolobus monocaudavirus 1 was capable of directly triggering the alternative pathway, we also demonstrate that the complement regulator factor H has no affinity for the viral surface, but factor H deposition is purely C3-dependent. This suggests that unlike some virulent pathogens Sulfolobus monocaudavirus 1 does not acquire factor H for protection. Complement activation with Sulfolobus monocaudavirus 1 also proceeds in murine sera through MBL-A/C as well as factor D-dependent manner, but C3 deficiency has no overall effect on viral clearance by organs of the reticuloendothelial system on intravenous injection. However, splenic deposition was significantly higher in C3 knockout animals compared with the corresponding wild type mice. We discuss the potential application of these viruses in biomedicine in relation to their complement activating properties.

Original language	English
Journal	Molecular Immunology
Volume	90
Pages (from-to)	273-279
Number of pages	7
ISSN	0161-5890
DOIs	https://doi.org/10.1016/j.molimm.2017.08.009
Publication status	Published - Oct 2017

Keywords

Complement system
Mannose-binding lectin
Nano-biotechnology
Reticuloendothelial system
Sulfolobus monocaudavirus 1
Sulfolobus spindle-shaped virus 2

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10.1016/j.molimm.2017.08.009

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@article{22a94f46fe4146e39b880ae03980967e,

title = "Interaction of extremophilic archaeal viruses with human and mouse complement system and viral biodistribution in mice",

abstract = "Archaeal viruses offer exceptional biophysical properties for modification and exploration of their potential in bionanotechnology, bioengineering and nanotherapeutic developments. However, the interaction of archaeal viruses with elements of the innate immune system has not been explored, which is a necessary prerequisite if their potential for biomedical applications to be realized. Here we show complement activation through lectin (via direct binding of MBL/MASPs) and alternative pathways by two extremophilic archaeal viruses (Sulfolobus monocaudavirus 1 and Sulfolobus spindle-shaped virus 2) in human serum. We further show some differences in initiation of complement activation pathways between these viruses. Since, Sulfolobus monocaudavirus 1 was capable of directly triggering the alternative pathway, we also demonstrate that the complement regulator factor H has no affinity for the viral surface, but factor H deposition is purely C3-dependent. This suggests that unlike some virulent pathogens Sulfolobus monocaudavirus 1 does not acquire factor H for protection. Complement activation with Sulfolobus monocaudavirus 1 also proceeds in murine sera through MBL-A/C as well as factor D-dependent manner, but C3 deficiency has no overall effect on viral clearance by organs of the reticuloendothelial system on intravenous injection. However, splenic deposition was significantly higher in C3 knockout animals compared with the corresponding wild type mice. We discuss the potential application of these viruses in biomedicine in relation to their complement activating properties.",

keywords = "Complement system, Mannose-binding lectin, Nano-biotechnology, Reticuloendothelial system, Sulfolobus monocaudavirus 1, Sulfolobus spindle-shaped virus 2",

author = "Linping Wu and Uldahl, {Kristine Buch} and Fangfang Chen and Halli Benasutti and Deborah Logvinski and Vivian Vu and Banda, {Nirmal K.} and Xu Peng and Dmitri Simberg and Moghimi, {Seyed Moein}",

year = "2017",

month = oct,

doi = "10.1016/j.molimm.2017.08.009",

language = "English",

volume = "90",

pages = "273--279",

journal = "Molecular Immunology",

issn = "0161-5890",

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T1 - Interaction of extremophilic archaeal viruses with human and mouse complement system and viral biodistribution in mice

AU - Wu, Linping

AU - Uldahl, Kristine Buch

AU - Chen, Fangfang

AU - Benasutti, Halli

AU - Logvinski, Deborah

AU - Vu, Vivian

AU - Banda, Nirmal K.

AU - Peng, Xu

AU - Simberg, Dmitri

AU - Moghimi, Seyed Moein

PY - 2017/10

Y1 - 2017/10

N2 - Archaeal viruses offer exceptional biophysical properties for modification and exploration of their potential in bionanotechnology, bioengineering and nanotherapeutic developments. However, the interaction of archaeal viruses with elements of the innate immune system has not been explored, which is a necessary prerequisite if their potential for biomedical applications to be realized. Here we show complement activation through lectin (via direct binding of MBL/MASPs) and alternative pathways by two extremophilic archaeal viruses (Sulfolobus monocaudavirus 1 and Sulfolobus spindle-shaped virus 2) in human serum. We further show some differences in initiation of complement activation pathways between these viruses. Since, Sulfolobus monocaudavirus 1 was capable of directly triggering the alternative pathway, we also demonstrate that the complement regulator factor H has no affinity for the viral surface, but factor H deposition is purely C3-dependent. This suggests that unlike some virulent pathogens Sulfolobus monocaudavirus 1 does not acquire factor H for protection. Complement activation with Sulfolobus monocaudavirus 1 also proceeds in murine sera through MBL-A/C as well as factor D-dependent manner, but C3 deficiency has no overall effect on viral clearance by organs of the reticuloendothelial system on intravenous injection. However, splenic deposition was significantly higher in C3 knockout animals compared with the corresponding wild type mice. We discuss the potential application of these viruses in biomedicine in relation to their complement activating properties.

AB - Archaeal viruses offer exceptional biophysical properties for modification and exploration of their potential in bionanotechnology, bioengineering and nanotherapeutic developments. However, the interaction of archaeal viruses with elements of the innate immune system has not been explored, which is a necessary prerequisite if their potential for biomedical applications to be realized. Here we show complement activation through lectin (via direct binding of MBL/MASPs) and alternative pathways by two extremophilic archaeal viruses (Sulfolobus monocaudavirus 1 and Sulfolobus spindle-shaped virus 2) in human serum. We further show some differences in initiation of complement activation pathways between these viruses. Since, Sulfolobus monocaudavirus 1 was capable of directly triggering the alternative pathway, we also demonstrate that the complement regulator factor H has no affinity for the viral surface, but factor H deposition is purely C3-dependent. This suggests that unlike some virulent pathogens Sulfolobus monocaudavirus 1 does not acquire factor H for protection. Complement activation with Sulfolobus monocaudavirus 1 also proceeds in murine sera through MBL-A/C as well as factor D-dependent manner, but C3 deficiency has no overall effect on viral clearance by organs of the reticuloendothelial system on intravenous injection. However, splenic deposition was significantly higher in C3 knockout animals compared with the corresponding wild type mice. We discuss the potential application of these viruses in biomedicine in relation to their complement activating properties.

KW - Complement system

KW - Mannose-binding lectin

KW - Nano-biotechnology

KW - Reticuloendothelial system

KW - Sulfolobus monocaudavirus 1

KW - Sulfolobus spindle-shaped virus 2

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DO - 10.1016/j.molimm.2017.08.009

M3 - Journal article

C2 - 28846925

AN - SCOPUS:85028343853

SN - 0161-5890

VL - 90

SP - 273

EP - 279

JO - Molecular Immunology

JF - Molecular Immunology

ER -

Interaction of extremophilic archaeal viruses with human and mouse complement system and viral biodistribution in mice

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Keywords

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