Interaction of barley powdery mildew effector candidate CSEP0055 with the defence protein PR17c

Wenjing Zhang; Carsten Pedersen; Mark Adrianus Cornelis J Kwaaitaal; Per L. Gregersen; Sara Melhedegård Mørch; Susanne Hanisch; Astrid Kristensen; Anja Thoe Fuglsang; David B. Collinge; Hans Thordal-Christensen

doi:10.1111/j.1364-3703.2012.00820.x

Interaction of barley powdery mildew effector candidate CSEP0055 with the defence protein PR17c

Wenjing Zhang, Carsten Pedersen, Mark Adrianus Cornelis J Kwaaitaal, Per L. Gregersen, Sara Melhedegård Mørch, Susanne Hanisch, Astrid Kristensen, Anja Thoe Fuglsang, David B. Collinge, Hans Thordal-Christensen

65 Citations (Scopus)

Abstract

A large number of effector candidates have been identified recently in powdery mildew fungi. However, their roles and how they perform their functions remain unresolved. In this study, we made use of host-induced gene silencing and confirmed that the secreted barley powdery mildew effector candidate, CSEP0055, contributes to the aggressiveness of the fungus. This result suggests that CSEP0055 is involved in the suppression of plant defence. A yeast two-hybrid screen indicated that CSEP0055 interacts with members of the barley pathogenesis-related protein families, PR1 and PR17. Interaction with PR17c was confirmed by bimolecular fluorescence complementation analyses. Down-regulation and over-expression of PR17c in epidermal cells of barley confirmed that this protein is important for penetration resistance against the powdery mildew fungus. In line with this, PR17c was found to be apoplastic, localizing to the papillae formed in response to this fungus. The CSEP0055 transcript did not start to accumulate until 24h after inoculation. This suggests that this gene is expressed too late to influence primary penetration events, but rather sustains the fungus at sites of secondary penetration, where PR17c appears to be able to accumulate.

Original language	English
Journal	Molecular Plant Pathology
Volume	13
Issue number	9
Pages (from-to)	1110-1119
Number of pages	10
ISSN	1464-6722
DOIs	https://doi.org/10.1111/j.1364-3703.2012.00820.x
Publication status	Published - Dec 2012

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@article{1d06b07642b74d2099515456f3bfffd8,

title = "Interaction of barley powdery mildew effector candidate CSEP0055 with the defence protein PR17c",

abstract = "A large number of effector candidates have been identified recently in powdery mildew fungi. However, their roles and how they perform their functions remain unresolved. In this study, we made use of host-induced gene silencing and confirmed that the secreted barley powdery mildew effector candidate, CSEP0055, contributes to the aggressiveness of the fungus. This result suggests that CSEP0055 is involved in the suppression of plant defence. A yeast two-hybrid screen indicated that CSEP0055 interacts with members of the barley pathogenesis-related protein families, PR1 and PR17. Interaction with PR17c was confirmed by bimolecular fluorescence complementation analyses. Down-regulation and over-expression of PR17c in epidermal cells of barley confirmed that this protein is important for penetration resistance against the powdery mildew fungus. In line with this, PR17c was found to be apoplastic, localizing to the papillae formed in response to this fungus. The CSEP0055 transcript did not start to accumulate until 24h after inoculation. This suggests that this gene is expressed too late to influence primary penetration events, but rather sustains the fungus at sites of secondary penetration, where PR17c appears to be able to accumulate.",

author = "Wenjing Zhang and Carsten Pedersen and Kwaaitaal, {Mark Adrianus Cornelis J} and Gregersen, {Per L.} and M{\o}rch, {Sara Melhedeg{\aa}rd} and Susanne Hanisch and Astrid Kristensen and Fuglsang, {Anja Thoe} and Collinge, {David B.} and Hans Thordal-Christensen",

year = "2012",

month = dec,

doi = "10.1111/j.1364-3703.2012.00820.x",

language = "English",

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journal = "Molecular Plant Pathology",

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TY - JOUR

T1 - Interaction of barley powdery mildew effector candidate CSEP0055 with the defence protein PR17c

AU - Zhang, Wenjing

AU - Pedersen, Carsten

AU - Kwaaitaal, Mark Adrianus Cornelis J

AU - Gregersen, Per L.

AU - Mørch, Sara Melhedegård

AU - Hanisch, Susanne

AU - Kristensen, Astrid

AU - Fuglsang, Anja Thoe

AU - Collinge, David B.

AU - Thordal-Christensen, Hans

PY - 2012/12

Y1 - 2012/12

N2 - A large number of effector candidates have been identified recently in powdery mildew fungi. However, their roles and how they perform their functions remain unresolved. In this study, we made use of host-induced gene silencing and confirmed that the secreted barley powdery mildew effector candidate, CSEP0055, contributes to the aggressiveness of the fungus. This result suggests that CSEP0055 is involved in the suppression of plant defence. A yeast two-hybrid screen indicated that CSEP0055 interacts with members of the barley pathogenesis-related protein families, PR1 and PR17. Interaction with PR17c was confirmed by bimolecular fluorescence complementation analyses. Down-regulation and over-expression of PR17c in epidermal cells of barley confirmed that this protein is important for penetration resistance against the powdery mildew fungus. In line with this, PR17c was found to be apoplastic, localizing to the papillae formed in response to this fungus. The CSEP0055 transcript did not start to accumulate until 24h after inoculation. This suggests that this gene is expressed too late to influence primary penetration events, but rather sustains the fungus at sites of secondary penetration, where PR17c appears to be able to accumulate.

AB - A large number of effector candidates have been identified recently in powdery mildew fungi. However, their roles and how they perform their functions remain unresolved. In this study, we made use of host-induced gene silencing and confirmed that the secreted barley powdery mildew effector candidate, CSEP0055, contributes to the aggressiveness of the fungus. This result suggests that CSEP0055 is involved in the suppression of plant defence. A yeast two-hybrid screen indicated that CSEP0055 interacts with members of the barley pathogenesis-related protein families, PR1 and PR17. Interaction with PR17c was confirmed by bimolecular fluorescence complementation analyses. Down-regulation and over-expression of PR17c in epidermal cells of barley confirmed that this protein is important for penetration resistance against the powdery mildew fungus. In line with this, PR17c was found to be apoplastic, localizing to the papillae formed in response to this fungus. The CSEP0055 transcript did not start to accumulate until 24h after inoculation. This suggests that this gene is expressed too late to influence primary penetration events, but rather sustains the fungus at sites of secondary penetration, where PR17c appears to be able to accumulate.

U2 - 10.1111/j.1364-3703.2012.00820.x

DO - 10.1111/j.1364-3703.2012.00820.x

M3 - Journal article

C2 - 22863200

SN - 1464-6722

VL - 13

SP - 1110

EP - 1119

JO - Molecular Plant Pathology

JF - Molecular Plant Pathology

IS - 9

ER -

Interaction of barley powdery mildew effector candidate CSEP0055 with the defence protein PR17c

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