Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens

Rie Satoh; Reiko Teshima; Kazumi Kitta; Gang-Hua Lang; Kathleen Schegg; Kenneth Blumenthal; Leslie Hicks; Bénédicte Labory-Carcenac; David Rouquié; Rod A Herman; Corinne Herouet-Guicheney; Gregory S Ladics; Scott McClain; Lars K Poulsen; Laura Privalle; Jason M Ward; Nancy Doerrer; Jean-Baptiste Rascle

doi:10.1080/09168451.2016.1206810

Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens

Rie Satoh, Reiko Teshima, Kazumi Kitta, Gang-Hua Lang, Kathleen Schegg, Kenneth Blumenthal, Leslie Hicks, Bénédicte Labory-Carcenac, David Rouquié, Rod A Herman, Corinne Herouet-Guicheney, Gregory S Ladics, Scott McClain, Lars K Poulsen, Laura Privalle, Jason M Ward, Nancy Doerrer, Jean-Baptiste Rascle

Department of Clinical Medicine

3 Citations (Scopus)

Abstract

In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the á-globulin, the 33 kDa glyoxalase I (Gly I), the 52-63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ringtrial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an interlaboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14-16 kDa á-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.

Original language	English
Journal	Bioscience, Biotechnology, and Biochemistry
Volume	80
Issue number	11
Pages (from-to)	2198-2207
Number of pages	10
ISSN	0916-8451
DOIs	https://doi.org/10.1080/09168451.2016.1206810
Publication status	Published - 2016

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Satoh, R., Teshima, R., Kitta, K., Lang, G.-H., Schegg, K., Blumenthal, K., Hicks, L., Labory-Carcenac, B., Rouquié, D., Herman, R. A., Herouet-Guicheney, C., Ladics, G. S., McClain, S., Poulsen, L. K., Privalle, L., Ward, J. M., Doerrer, N., & Rascle, J.-B. (2016). Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens. Bioscience, Biotechnology, and Biochemistry, 80(11), 2198-2207. https://doi.org/10.1080/09168451.2016.1206810

Satoh, R, Teshima, R, Kitta, K, Lang, G-H, Schegg, K, Blumenthal, K, Hicks, L, Labory-Carcenac, B, Rouquié, D, Herman, RA, Herouet-Guicheney, C, Ladics, GS, McClain, S, Poulsen, LK, Privalle, L, Ward, JM, Doerrer, N & Rascle, J-B 2016, 'Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens', Bioscience, Biotechnology, and Biochemistry, vol. 80, no. 11, pp. 2198-2207. https://doi.org/10.1080/09168451.2016.1206810

@article{70ea5c5ae29c4000a5dc2f9150b7e50d,

title = "Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens",

abstract = "In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the {\'a}-globulin, the 33 kDa glyoxalase I (Gly I), the 52-63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ringtrial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an interlaboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14-16 kDa {\'a}-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.",

author = "Rie Satoh and Reiko Teshima and Kazumi Kitta and Gang-Hua Lang and Kathleen Schegg and Kenneth Blumenthal and Leslie Hicks and B{\'e}n{\'e}dicte Labory-Carcenac and David Rouqui{\'e} and Herman, {Rod A} and Corinne Herouet-Guicheney and Ladics, {Gregory S} and Scott McClain and Poulsen, {Lars K} and Laura Privalle and Ward, {Jason M} and Nancy Doerrer and Jean-Baptiste Rascle",

year = "2016",

doi = "10.1080/09168451.2016.1206810",

language = "English",

volume = "80",

pages = "2198--2207",

journal = "Bioscience, Biotechnology, and Biochemistry",

issn = "0916-8451",

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TY - JOUR

T1 - Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens

AU - Satoh, Rie

AU - Teshima, Reiko

AU - Kitta, Kazumi

AU - Lang, Gang-Hua

AU - Schegg, Kathleen

AU - Blumenthal, Kenneth

AU - Hicks, Leslie

AU - Labory-Carcenac, Bénédicte

AU - Rouquié, David

AU - Herman, Rod A

AU - Herouet-Guicheney, Corinne

AU - Ladics, Gregory S

AU - McClain, Scott

AU - Poulsen, Lars K

AU - Privalle, Laura

AU - Ward, Jason M

AU - Doerrer, Nancy

AU - Rascle, Jean-Baptiste

PY - 2016

Y1 - 2016

N2 - In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the á-globulin, the 33 kDa glyoxalase I (Gly I), the 52-63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ringtrial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an interlaboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14-16 kDa á-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.

AB - In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the á-globulin, the 33 kDa glyoxalase I (Gly I), the 52-63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ringtrial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an interlaboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14-16 kDa á-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.

U2 - 10.1080/09168451.2016.1206810

DO - 10.1080/09168451.2016.1206810

M3 - Journal article

C2 - 27399872

SN - 0916-8451

VL - 80

SP - 2198

EP - 2207

JO - Bioscience, Biotechnology, and Biochemistry

JF - Bioscience, Biotechnology, and Biochemistry

IS - 11

ER -

Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens

Abstract

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