Inter-correlated gut microbiota and SCFAs changes upon antibiotics exposure links with rapid body-mass gain in weaned piglet model

Lianqiang Che; Qi Hu; Ru Wang; Du Zhang; Cong Liu; Yihe Zhang; Guizhong Xin; Zhengfeng Fang; Yan Lin; Shengyu Xu; Bin Feng; Daiwen Chen; De Wu; Fei Gao

doi:10.1016/j.jnutbio.2019.108246

Inter-correlated gut microbiota and SCFAs changes upon antibiotics exposure links with rapid body-mass gain in weaned piglet model

Lianqiang Che^*, Qi Hu, Ru Wang, Du Zhang, Cong Liu, Yihe Zhang, Guizhong Xin, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Daiwen Chen, De Wu, Fei Gao

^*Corresponding author for this work

6 Citations (Scopus)

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Abstract

The risk of overweight or obesity in association with early exposure of antibiotics remains an important public issue for health-care of children. Low-dose antibiotics (LDA) have been widely used to enhance growth rate of pigs, providing a good animal model to study the underlying mechanism. In present study, 28 female piglets, weaned at 21 d, were randomly classified into two groups, receiving either a control diet or a diet supplemented with LDA for 4 weeks. The total bacterial load and intestinal microbiota were determined by qPCR and 16S rRNA amplicon sequencing. UPLC-QTRAP-MS/MS and RNA-seq were further used to determine the colonic SCFAs and transcriptomes. Results showed that LDA significantly increased growth rate and food intake. The F/B index, Methanosphaera species, and the pathway of “carbohydrate metabolism” were improved by LDA exposure, indicating the better carbohydrate degradation and energy utilization. Furthermore, correlation analysis indicated the microbial community contributing to SCFAs production was enriched upon LDA exposure, associating with increased concentrations of short-chain and branched-chain fatty acids (caproate, 2-methyl butyrate and 4-methyl valerate). A multivariate linear fitting model analysis highlighted that caproate was positively correlated with two genera (Faecalibacterium and Allisonella) and four differentially expressed genes (ZNF134, TBX5, NEU4 and SEMA6D), which were all significantly increased upon LDA exposure. Collectively, our study indicates that the growth-promoting effect of LDA exposure in early life is associated with the shifts of colonic microbiota to increase utilization of carbohydrates and energy, enhanced SCFAs production and colonic functions.

Original language	English
Article number	108246
Journal	Journal of Nutritional Biochemistry
Volume	74
Number of pages	10
ISSN	0955-2863
DOIs	https://doi.org/10.1016/j.jnutbio.2019.108246
Publication status	Published - 2019

Keywords

Antibiotics
Intestine
Microbiota
Obesity
Overweight

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jnutbio.2019.108246Licence: CC BY-NC-ND

Inter-correlated gut microbiota and SCFAs changes upon antibiotics exposure links with rapid body-mass gain in weaned piglet modelFinal published version, 1.04 MBLicence: CC BY-NC-ND

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Che, L., Hu, Q., Wang, R., Zhang, D., Liu, C., Zhang, Y., Xin, G., Fang, Z., Lin, Y., Xu, S., Feng, B., Chen, D., Wu, D., & Gao, F. (2019). Inter-correlated gut microbiota and SCFAs changes upon antibiotics exposure links with rapid body-mass gain in weaned piglet model. Journal of Nutritional Biochemistry, 74, Article 108246. https://doi.org/10.1016/j.jnutbio.2019.108246

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title = "Inter-correlated gut microbiota and SCFAs changes upon antibiotics exposure links with rapid body-mass gain in weaned piglet model",

abstract = "The risk of overweight or obesity in association with early exposure of antibiotics remains an important public issue for health-care of children. Low-dose antibiotics (LDA) have been widely used to enhance growth rate of pigs, providing a good animal model to study the underlying mechanism. In present study, 28 female piglets, weaned at 21 d, were randomly classified into two groups, receiving either a control diet or a diet supplemented with LDA for 4 weeks. The total bacterial load and intestinal microbiota were determined by qPCR and 16S rRNA amplicon sequencing. UPLC-QTRAP-MS/MS and RNA-seq were further used to determine the colonic SCFAs and transcriptomes. Results showed that LDA significantly increased growth rate and food intake. The F/B index, Methanosphaera species, and the pathway of “carbohydrate metabolism” were improved by LDA exposure, indicating the better carbohydrate degradation and energy utilization. Furthermore, correlation analysis indicated the microbial community contributing to SCFAs production was enriched upon LDA exposure, associating with increased concentrations of short-chain and branched-chain fatty acids (caproate, 2-methyl butyrate and 4-methyl valerate). A multivariate linear fitting model analysis highlighted that caproate was positively correlated with two genera (Faecalibacterium and Allisonella) and four differentially expressed genes (ZNF134, TBX5, NEU4 and SEMA6D), which were all significantly increased upon LDA exposure. Collectively, our study indicates that the growth-promoting effect of LDA exposure in early life is associated with the shifts of colonic microbiota to increase utilization of carbohydrates and energy, enhanced SCFAs production and colonic functions.",

keywords = "Antibiotics, Intestine, Microbiota, Obesity, Overweight",

author = "Lianqiang Che and Qi Hu and Ru Wang and Du Zhang and Cong Liu and Yihe Zhang and Guizhong Xin and Zhengfeng Fang and Yan Lin and Shengyu Xu and Bin Feng and Daiwen Chen and De Wu and Fei Gao",

year = "2019",

doi = "10.1016/j.jnutbio.2019.108246",

language = "English",

volume = "74",

journal = "Journal of Nutritional Biochemistry",

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TY - JOUR

T1 - Inter-correlated gut microbiota and SCFAs changes upon antibiotics exposure links with rapid body-mass gain in weaned piglet model

AU - Che, Lianqiang

AU - Hu, Qi

AU - Wang, Ru

AU - Zhang, Du

AU - Liu, Cong

AU - Zhang, Yihe

AU - Xin, Guizhong

AU - Fang, Zhengfeng

AU - Lin, Yan

AU - Xu, Shengyu

AU - Feng, Bin

AU - Chen, Daiwen

AU - Wu, De

AU - Gao, Fei

PY - 2019

Y1 - 2019

N2 - The risk of overweight or obesity in association with early exposure of antibiotics remains an important public issue for health-care of children. Low-dose antibiotics (LDA) have been widely used to enhance growth rate of pigs, providing a good animal model to study the underlying mechanism. In present study, 28 female piglets, weaned at 21 d, were randomly classified into two groups, receiving either a control diet or a diet supplemented with LDA for 4 weeks. The total bacterial load and intestinal microbiota were determined by qPCR and 16S rRNA amplicon sequencing. UPLC-QTRAP-MS/MS and RNA-seq were further used to determine the colonic SCFAs and transcriptomes. Results showed that LDA significantly increased growth rate and food intake. The F/B index, Methanosphaera species, and the pathway of “carbohydrate metabolism” were improved by LDA exposure, indicating the better carbohydrate degradation and energy utilization. Furthermore, correlation analysis indicated the microbial community contributing to SCFAs production was enriched upon LDA exposure, associating with increased concentrations of short-chain and branched-chain fatty acids (caproate, 2-methyl butyrate and 4-methyl valerate). A multivariate linear fitting model analysis highlighted that caproate was positively correlated with two genera (Faecalibacterium and Allisonella) and four differentially expressed genes (ZNF134, TBX5, NEU4 and SEMA6D), which were all significantly increased upon LDA exposure. Collectively, our study indicates that the growth-promoting effect of LDA exposure in early life is associated with the shifts of colonic microbiota to increase utilization of carbohydrates and energy, enhanced SCFAs production and colonic functions.

AB - The risk of overweight or obesity in association with early exposure of antibiotics remains an important public issue for health-care of children. Low-dose antibiotics (LDA) have been widely used to enhance growth rate of pigs, providing a good animal model to study the underlying mechanism. In present study, 28 female piglets, weaned at 21 d, were randomly classified into two groups, receiving either a control diet or a diet supplemented with LDA for 4 weeks. The total bacterial load and intestinal microbiota were determined by qPCR and 16S rRNA amplicon sequencing. UPLC-QTRAP-MS/MS and RNA-seq were further used to determine the colonic SCFAs and transcriptomes. Results showed that LDA significantly increased growth rate and food intake. The F/B index, Methanosphaera species, and the pathway of “carbohydrate metabolism” were improved by LDA exposure, indicating the better carbohydrate degradation and energy utilization. Furthermore, correlation analysis indicated the microbial community contributing to SCFAs production was enriched upon LDA exposure, associating with increased concentrations of short-chain and branched-chain fatty acids (caproate, 2-methyl butyrate and 4-methyl valerate). A multivariate linear fitting model analysis highlighted that caproate was positively correlated with two genera (Faecalibacterium and Allisonella) and four differentially expressed genes (ZNF134, TBX5, NEU4 and SEMA6D), which were all significantly increased upon LDA exposure. Collectively, our study indicates that the growth-promoting effect of LDA exposure in early life is associated with the shifts of colonic microbiota to increase utilization of carbohydrates and energy, enhanced SCFAs production and colonic functions.

KW - Antibiotics

KW - Intestine

KW - Microbiota

KW - Obesity

KW - Overweight

U2 - 10.1016/j.jnutbio.2019.108246

DO - 10.1016/j.jnutbio.2019.108246

M3 - Journal article

C2 - 31671360

AN - SCOPUS:85074142870

SN - 0955-2863

VL - 74

JO - Journal of Nutritional Biochemistry

JF - Journal of Nutritional Biochemistry

M1 - 108246

ER -

Inter-correlated gut microbiota and SCFAs changes upon antibiotics exposure links with rapid body-mass gain in weaned piglet model

Abstract

Keywords

UN SDGs

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