Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes

Martin Thomassen; Thomas Gunnar Petursson Gunnarsson; Peter Møller Christensen; Davor Pavlovic; Michael Jonathan Shattock; Jens Bangsbo

doi:10.1152/ajpregu.00081.2015

Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes

Martin Thomassen, Thomas Gunnar Petursson Gunnarsson, Peter Møller Christensen, Davor Pavlovic, Michael Jonathan Shattock, Jens Bangsbo

The August Krogh Section for Human Physiology

12 Citations (Scopus)

Abstract

The present study examined the effect of intensive training in combination with marked reduction in training volume on phospholemman (FXYD1) expression and phosphorylation at rest and during exercise. Eight well-trained cyclists replaced their regular training with speed-endurance training (10 –12 × ~30-s sprints) two or three times per week and aerobic high-intensity training (4 –5 × 3– 4 min at 90–95% of peak aerobic power output) 1–2 times per week for 7 wk and reduced the training volume by 70%. Muscle biopsies were obtained before and during a repeated high-intensity exercise protocol, and protein expression and phosphorylation were determined by Western blot analysis. Expression of FXYD1 (30%), actin (40%), mammalian target of rapamycin (mTOR) (12%), phospholamban (PLN) (16%), and Ca²⁺/calmodulin-dependent protein kinase II (CaMKII) γ/δ (25%) was higher (P < 0.05) than before the training intervention. In addition, after the intervention, nonspecific FXYD1 phosphorylation was higher (P < 0.05) at rest and during exercise, mainly achieved by an increased FXYD1 Ser-68 phosphorylation, compared with before the intervention. CaMKII, Thr-287, and eukaryotic elongation factor 2 Thr-56 phosphorylation at rest and during exercise, overall PKCα/β, Thr-638/641, and mTOR Ser-2448 phosphorylation during repeated intense exercise as well as resting PLN Thr-17 phosphorylation were also higher (P < 0.05) compared with before the intervention period. Thus, a period of high-intensity training with reduced training volume increases expression and phosphorylation levels of FXYD1, which may affect Na⁺/K⁺ pump activity and muscle K⁺ homeostasis during intense exercise. Furthermore, higher expression of CaMKII and PLN, as well as increased phosphorylation of CaMKII Thr-287 may have improved intracellular Ca²⁺ handling.

Original language	English
Journal	American Journal of Physiology: Regulatory, Integrative and Comparative Physiology
Volume	310
Issue number	7
Pages (from-to)	R659-R669
Number of pages	11
ISSN	0363-6119
DOIs	https://doi.org/10.1152/ajpregu.00081.2015
Publication status	Published - Apr 2016

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Thomassen, M., Gunnarsson, T. G. P., Christensen, P. M., Pavlovic, D., Shattock, M. J., & Bangsbo, J. (2016). Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 310(7), R659-R669. https://doi.org/10.1152/ajpregu.00081.2015

Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes. / Thomassen, Martin; Gunnarsson, Thomas Gunnar Petursson; Christensen, Peter Møller et al.
In: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Vol. 310, No. 7, 04.2016, p. R659-R669.

Research output: Contribution to journal › Journal article › Research › peer-review

Thomassen, M, Gunnarsson, TGP, Christensen, PM, Pavlovic, D, Shattock, MJ & Bangsbo, J 2016, 'Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes', American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, vol. 310, no. 7, pp. R659-R669. https://doi.org/10.1152/ajpregu.00081.2015

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title = "Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes",

abstract = "The present study examined the effect of intensive training in combination with marked reduction in training volume on phospholemman (FXYD1) expression and phosphorylation at rest and during exercise. Eight well-trained cyclists replaced their regular training with speed-endurance training (10 –12 × ~30-s sprints) two or three times per week and aerobic high-intensity training (4 –5 × 3– 4 min at 90–95% of peak aerobic power output) 1–2 times per week for 7 wk and reduced the training volume by 70%. Muscle biopsies were obtained before and during a repeated high-intensity exercise protocol, and protein expression and phosphorylation were determined by Western blot analysis. Expression of FXYD1 (30%), actin (40%), mammalian target of rapamycin (mTOR) (12%), phospholamban (PLN) (16%), and Ca2+/calmodulin-dependent protein kinase II (CaMKII) γ/δ (25%) was higher (P < 0.05) than before the training intervention. In addition, after the intervention, nonspecific FXYD1 phosphorylation was higher (P < 0.05) at rest and during exercise, mainly achieved by an increased FXYD1 Ser-68 phosphorylation, compared with before the intervention. CaMKII, Thr-287, and eukaryotic elongation factor 2 Thr-56 phosphorylation at rest and during exercise, overall PKCα/β, Thr-638/641, and mTOR Ser-2448 phosphorylation during repeated intense exercise as well as resting PLN Thr-17 phosphorylation were also higher (P < 0.05) compared with before the intervention period. Thus, a period of high-intensity training with reduced training volume increases expression and phosphorylation levels of FXYD1, which may affect Na+/K+ pump activity and muscle K+ homeostasis during intense exercise. Furthermore, higher expression of CaMKII and PLN, as well as increased phosphorylation of CaMKII Thr-287 may have improved intracellular Ca2+ handling.",

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T1 - Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes

AU - Thomassen, Martin

AU - Gunnarsson, Thomas Gunnar Petursson

AU - Christensen, Peter Møller

AU - Pavlovic, Davor

AU - Shattock, Michael Jonathan

AU - Bangsbo, Jens

N1 - CURIS 2016 NEXS 103

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N2 - The present study examined the effect of intensive training in combination with marked reduction in training volume on phospholemman (FXYD1) expression and phosphorylation at rest and during exercise. Eight well-trained cyclists replaced their regular training with speed-endurance training (10 –12 × ~30-s sprints) two or three times per week and aerobic high-intensity training (4 –5 × 3– 4 min at 90–95% of peak aerobic power output) 1–2 times per week for 7 wk and reduced the training volume by 70%. Muscle biopsies were obtained before and during a repeated high-intensity exercise protocol, and protein expression and phosphorylation were determined by Western blot analysis. Expression of FXYD1 (30%), actin (40%), mammalian target of rapamycin (mTOR) (12%), phospholamban (PLN) (16%), and Ca2+/calmodulin-dependent protein kinase II (CaMKII) γ/δ (25%) was higher (P < 0.05) than before the training intervention. In addition, after the intervention, nonspecific FXYD1 phosphorylation was higher (P < 0.05) at rest and during exercise, mainly achieved by an increased FXYD1 Ser-68 phosphorylation, compared with before the intervention. CaMKII, Thr-287, and eukaryotic elongation factor 2 Thr-56 phosphorylation at rest and during exercise, overall PKCα/β, Thr-638/641, and mTOR Ser-2448 phosphorylation during repeated intense exercise as well as resting PLN Thr-17 phosphorylation were also higher (P < 0.05) compared with before the intervention period. Thus, a period of high-intensity training with reduced training volume increases expression and phosphorylation levels of FXYD1, which may affect Na+/K+ pump activity and muscle K+ homeostasis during intense exercise. Furthermore, higher expression of CaMKII and PLN, as well as increased phosphorylation of CaMKII Thr-287 may have improved intracellular Ca2+ handling.

AB - The present study examined the effect of intensive training in combination with marked reduction in training volume on phospholemman (FXYD1) expression and phosphorylation at rest and during exercise. Eight well-trained cyclists replaced their regular training with speed-endurance training (10 –12 × ~30-s sprints) two or three times per week and aerobic high-intensity training (4 –5 × 3– 4 min at 90–95% of peak aerobic power output) 1–2 times per week for 7 wk and reduced the training volume by 70%. Muscle biopsies were obtained before and during a repeated high-intensity exercise protocol, and protein expression and phosphorylation were determined by Western blot analysis. Expression of FXYD1 (30%), actin (40%), mammalian target of rapamycin (mTOR) (12%), phospholamban (PLN) (16%), and Ca2+/calmodulin-dependent protein kinase II (CaMKII) γ/δ (25%) was higher (P < 0.05) than before the training intervention. In addition, after the intervention, nonspecific FXYD1 phosphorylation was higher (P < 0.05) at rest and during exercise, mainly achieved by an increased FXYD1 Ser-68 phosphorylation, compared with before the intervention. CaMKII, Thr-287, and eukaryotic elongation factor 2 Thr-56 phosphorylation at rest and during exercise, overall PKCα/β, Thr-638/641, and mTOR Ser-2448 phosphorylation during repeated intense exercise as well as resting PLN Thr-17 phosphorylation were also higher (P < 0.05) compared with before the intervention period. Thus, a period of high-intensity training with reduced training volume increases expression and phosphorylation levels of FXYD1, which may affect Na+/K+ pump activity and muscle K+ homeostasis during intense exercise. Furthermore, higher expression of CaMKII and PLN, as well as increased phosphorylation of CaMKII Thr-287 may have improved intracellular Ca2+ handling.

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DO - 10.1152/ajpregu.00081.2015

M3 - Journal article

C2 - 26791827

SN - 0363-6119

VL - 310

SP - R659-R669

JO - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

JF - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

IS - 7

ER -

Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes

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