Instant infusion pasteurisation of bovine milk. II. Effects on indigenous milk enzymes activity and whey protein denaturation

Marianne Hammershøj, Anni Bygvrå Hougaard, Jannie Steensig Vestergaard, Ole Poulsen, Richard Ipsen

    11 Citations (Scopus)

    Abstract

    Direct heat treatment of two milk types, skimmed and nonstandardised full-fat, was performed by instant steam infusion and compared with indirect heating. Infusion conditions were temperatures of 72-120°C combined with holding times of 100-700 ms, and indirect heat conditions were 72°C/15 s and 85°C/30 s. The activity of indigenous enzymes such as alkaline phosphatase, lactoperoxidase, xanthine oxidase and γ-glutamyl transpeptidase was evaluated. Infusion temperature was the main determinant of inactivation. Whey protein denaturation represented by β-lactoglobulin increased significantly with infusion temperature. The nonstandardised milk had a higher denaturation rate than skimmed milk. The effect of instant infusion on pH and milk fat globule size in relation to whey protein denaturation and association is discussed.

    Original languageEnglish
    JournalInternational Journal of Dairy Technology
    Volume63
    Issue number2
    Pages (from-to)197-208
    Number of pages12
    ISSN1364-727X
    DOIs
    Publication statusPublished - May 2010

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