TY - JOUR
T1 - Instant infusion pasteurisation of bovine milk. II.
T2 - Effects on indigenous milk enzymes activity and whey protein denaturation
AU - Hammershøj, Marianne
AU - Hougaard, Anni Bygvrå
AU - Vestergaard, Jannie Steensig
AU - Poulsen, Ole
AU - Ipsen, Richard
PY - 2010/5
Y1 - 2010/5
N2 - Direct heat treatment of two milk types, skimmed and nonstandardised full-fat, was performed by instant steam infusion and compared with indirect heating. Infusion conditions were temperatures of 72-120°C combined with holding times of 100-700 ms, and indirect heat conditions were 72°C/15 s and 85°C/30 s. The activity of indigenous enzymes such as alkaline phosphatase, lactoperoxidase, xanthine oxidase and γ-glutamyl transpeptidase was evaluated. Infusion temperature was the main determinant of inactivation. Whey protein denaturation represented by β-lactoglobulin increased significantly with infusion temperature. The nonstandardised milk had a higher denaturation rate than skimmed milk. The effect of instant infusion on pH and milk fat globule size in relation to whey protein denaturation and association is discussed.
AB - Direct heat treatment of two milk types, skimmed and nonstandardised full-fat, was performed by instant steam infusion and compared with indirect heating. Infusion conditions were temperatures of 72-120°C combined with holding times of 100-700 ms, and indirect heat conditions were 72°C/15 s and 85°C/30 s. The activity of indigenous enzymes such as alkaline phosphatase, lactoperoxidase, xanthine oxidase and γ-glutamyl transpeptidase was evaluated. Infusion temperature was the main determinant of inactivation. Whey protein denaturation represented by β-lactoglobulin increased significantly with infusion temperature. The nonstandardised milk had a higher denaturation rate than skimmed milk. The effect of instant infusion on pH and milk fat globule size in relation to whey protein denaturation and association is discussed.
U2 - 10.1111/j.1471-0307.2010.00583.x
DO - 10.1111/j.1471-0307.2010.00583.x
M3 - Journal article
SN - 1364-727X
VL - 63
SP - 197
EP - 208
JO - International Journal of Dairy Technology
JF - International Journal of Dairy Technology
IS - 2
ER -