Inhibition of hippocampal synaptic transmission by impairment of Ral function.

Björn Owe-Larsson; Esteban Chaves-Olarte; Ashok Chauhan; Ole Kjaerulff; Johan Brask; Monica Thelestam; Lennart Brodin; Peter Löw

Inhibition of hippocampal synaptic transmission by impairment of Ral function.

Björn Owe-Larsson, Esteban Chaves-Olarte, Ashok Chauhan, Ole Kjaerulff, Johan Brask, Monica Thelestam, Lennart Brodin, Peter Löw

Department of Neuroscience

4 Citations (Scopus)

Abstract

Large clostridial cytotoxins and protein overexpression were used to probe for involvement of Ras-related GTPases (guanosine triphosphate) in synaptic transmission in cultured rat hippocampal neurons. The toxins TcdA-10463 (inactivates Rho, Rac, Cdc42, Rap) and TcsL-1522 (inactivates Ral, Rac, Ras, R-Ras, Rap) both inhibited autaptic responses. In a proportion of the neurons (25%, TcdA-10463; 54%, TcsL-1522), the inhibition was associated with a shift from activity-dependent depression to facilitation, indicating that the synaptic release probability was reduced. Overexpression of a dominant negative Ral mutant, Ral A28N, caused a strong inhibition of autaptic responses, which was associated with a shift to facilitation in a majority (80%) of the neurons. These results indicate that Ral, along with at least one other non-Rab GTPase, participates in presynaptic regulation in hippocampal neurons.

Original language	English
Journal	NeuroReport
Volume	16
Issue number	16
Pages (from-to)	1805-8
Number of pages	3
ISSN	0959-4965
Publication status	Published - 2005

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@article{41a54c00752311dd8d9f000ea68e967b,

title = "Inhibition of hippocampal synaptic transmission by impairment of Ral function.",

abstract = "Large clostridial cytotoxins and protein overexpression were used to probe for involvement of Ras-related GTPases (guanosine triphosphate) in synaptic transmission in cultured rat hippocampal neurons. The toxins TcdA-10463 (inactivates Rho, Rac, Cdc42, Rap) and TcsL-1522 (inactivates Ral, Rac, Ras, R-Ras, Rap) both inhibited autaptic responses. In a proportion of the neurons (25%, TcdA-10463; 54%, TcsL-1522), the inhibition was associated with a shift from activity-dependent depression to facilitation, indicating that the synaptic release probability was reduced. Overexpression of a dominant negative Ral mutant, Ral A28N, caused a strong inhibition of autaptic responses, which was associated with a shift to facilitation in a majority (80%) of the neurons. These results indicate that Ral, along with at least one other non-Rab GTPase, participates in presynaptic regulation in hippocampal neurons.",

author = "Bj{\"o}rn Owe-Larsson and Esteban Chaves-Olarte and Ashok Chauhan and Ole Kjaerulff and Johan Brask and Monica Thelestam and Lennart Brodin and Peter L{\"o}w",

note = "Keywords: Alanine; Animals; Arabidopsis Proteins; Asparagine; Bacterial Toxins; Blotting, Western; Carbon Isotopes; Cells, Cultured; Drug Interactions; Electric Stimulation; Enterotoxins; Excitatory Amino Acid Agonists; Gene Expression Regulation; Glucose; Hippocampus; Membrane Potentials; Mutation; Neural Inhibition; Neurons; Patch-Clamp Techniques; Rats; Synaptic Transmission; Toxins, Biological; Transfection; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; gamma-Aminobutyric Acid; ral GTP-Binding Proteins",

year = "2005",

language = "English",

volume = "16",

pages = "1805--8",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams & Wilkins",

number = "16",

}

TY - JOUR

T1 - Inhibition of hippocampal synaptic transmission by impairment of Ral function.

AU - Owe-Larsson, Björn

AU - Chaves-Olarte, Esteban

AU - Chauhan, Ashok

AU - Kjaerulff, Ole

AU - Brask, Johan

AU - Thelestam, Monica

AU - Brodin, Lennart

AU - Löw, Peter

N1 - Keywords: Alanine; Animals; Arabidopsis Proteins; Asparagine; Bacterial Toxins; Blotting, Western; Carbon Isotopes; Cells, Cultured; Drug Interactions; Electric Stimulation; Enterotoxins; Excitatory Amino Acid Agonists; Gene Expression Regulation; Glucose; Hippocampus; Membrane Potentials; Mutation; Neural Inhibition; Neurons; Patch-Clamp Techniques; Rats; Synaptic Transmission; Toxins, Biological; Transfection; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; gamma-Aminobutyric Acid; ral GTP-Binding Proteins

PY - 2005

Y1 - 2005

N2 - Large clostridial cytotoxins and protein overexpression were used to probe for involvement of Ras-related GTPases (guanosine triphosphate) in synaptic transmission in cultured rat hippocampal neurons. The toxins TcdA-10463 (inactivates Rho, Rac, Cdc42, Rap) and TcsL-1522 (inactivates Ral, Rac, Ras, R-Ras, Rap) both inhibited autaptic responses. In a proportion of the neurons (25%, TcdA-10463; 54%, TcsL-1522), the inhibition was associated with a shift from activity-dependent depression to facilitation, indicating that the synaptic release probability was reduced. Overexpression of a dominant negative Ral mutant, Ral A28N, caused a strong inhibition of autaptic responses, which was associated with a shift to facilitation in a majority (80%) of the neurons. These results indicate that Ral, along with at least one other non-Rab GTPase, participates in presynaptic regulation in hippocampal neurons.

AB - Large clostridial cytotoxins and protein overexpression were used to probe for involvement of Ras-related GTPases (guanosine triphosphate) in synaptic transmission in cultured rat hippocampal neurons. The toxins TcdA-10463 (inactivates Rho, Rac, Cdc42, Rap) and TcsL-1522 (inactivates Ral, Rac, Ras, R-Ras, Rap) both inhibited autaptic responses. In a proportion of the neurons (25%, TcdA-10463; 54%, TcsL-1522), the inhibition was associated with a shift from activity-dependent depression to facilitation, indicating that the synaptic release probability was reduced. Overexpression of a dominant negative Ral mutant, Ral A28N, caused a strong inhibition of autaptic responses, which was associated with a shift to facilitation in a majority (80%) of the neurons. These results indicate that Ral, along with at least one other non-Rab GTPase, participates in presynaptic regulation in hippocampal neurons.

M3 - Journal article

C2 - 16237331

SN - 0959-4965

VL - 16

SP - 1805

EP - 1808

JO - NeuroReport

JF - NeuroReport

IS - 16

ER -

Inhibition of hippocampal synaptic transmission by impairment of Ral function.

Abstract

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