Infusion of ATP increases leg oxygen delivery but not oxygen uptake in the initial phase of intense knee-extensor exercise in humans

TY - JOUR

T1 - Infusion of ATP increases leg oxygen delivery but not oxygen uptake in the initial phase of intense knee-extensor exercise in humans

AU - Nyberg, Michael Permin

AU - Christensen, Peter Møller

AU - Mortensen, Stefan Peter

AU - Hellsten, Ylva

AU - Bangsbo, Jens

N1 - CURIS 2014 NEXS 294

PY - 2014

Y1 - 2014

N2 - The present study examined whether an increase in leg blood flow and oxygen delivery at the onset of intense exercise would speed the rate of rise in leg oxygen uptake. Nine healthy men (25 ± 1 years old, mean ± SEM) performed one-leg knee-extensor exercise (62 ± 3 W, 86 ± 3% of incremental test peak power) for 4 min during a control setting (CON) and with infusion of ATP into the femoral artery in order to increase blood flow before and during exercise. In the presence of ATP, femoral arterial blood flow and O2 delivery were higher (P < 0.001) at the onset of exercise and throughout exercise (femoral arterial blood flow after 10 s, 5.1 ± 0.5 versus 2.7 ± 0.3 l min-1; after 45 s, 6.0 ± 0.5 versus 4.1 ± 0.4 l min-1; after 90 s, 6.6 ± 0.6 versus 4.5 ± 0.4 l min-1; and after 240 s, 7.0 ± 0.6 versus 5.1 ± 0.3 l min-1 in ATP and CON conditions, respectively). Leg oxygen uptake was not different in ATP and CON conditions during the first 20 s of exercise but was lower (P < 0.05) in the ATP compared with CON conditions after 30 s and until the end of exercise (30 s, 436 ± 42 versus 549 ± 45 ml min-1; and 240 s, 705 ± 31 versus 814 ± 59 ml min-1 in ATP and CON, respectively). Lactate release was lower after 60, 120 and 180 s of exercise with ATP infusion. These results suggest that O2 delivery is not limiting the rise in skeletal muscle oxygen uptake in the initial phase of intense exercise.

AB - The present study examined whether an increase in leg blood flow and oxygen delivery at the onset of intense exercise would speed the rate of rise in leg oxygen uptake. Nine healthy men (25 ± 1 years old, mean ± SEM) performed one-leg knee-extensor exercise (62 ± 3 W, 86 ± 3% of incremental test peak power) for 4 min during a control setting (CON) and with infusion of ATP into the femoral artery in order to increase blood flow before and during exercise. In the presence of ATP, femoral arterial blood flow and O2 delivery were higher (P < 0.001) at the onset of exercise and throughout exercise (femoral arterial blood flow after 10 s, 5.1 ± 0.5 versus 2.7 ± 0.3 l min-1; after 45 s, 6.0 ± 0.5 versus 4.1 ± 0.4 l min-1; after 90 s, 6.6 ± 0.6 versus 4.5 ± 0.4 l min-1; and after 240 s, 7.0 ± 0.6 versus 5.1 ± 0.3 l min-1 in ATP and CON conditions, respectively). Leg oxygen uptake was not different in ATP and CON conditions during the first 20 s of exercise but was lower (P < 0.05) in the ATP compared with CON conditions after 30 s and until the end of exercise (30 s, 436 ± 42 versus 549 ± 45 ml min-1; and 240 s, 705 ± 31 versus 814 ± 59 ml min-1 in ATP and CON, respectively). Lactate release was lower after 60, 120 and 180 s of exercise with ATP infusion. These results suggest that O2 delivery is not limiting the rise in skeletal muscle oxygen uptake in the initial phase of intense exercise.

U2 - 10.1113/expphysiol.2014.081141

DO - 10.1113/expphysiol.2014.081141

M3 - Journal article

C2 - 25085840

SN - 0958-0670

VL - 99

SP - 1399

EP - 1408

JO - Experimental Physiology

JF - Experimental Physiology

IS - 10

ER -