TY - JOUR
T1 - Influence of dynamic flow environment on nanoparticle-protein corona
T2 - From protein patterns to uptake in cancer cells
AU - Palchetti, Sara
AU - Pozzi, Daniela
AU - Capriotti, Anna Laura
AU - Barbera, Giorgia La
AU - Chiozzi, Riccardo Zenezini
AU - Digiacomo, Luca
AU - Peruzzi, Giovanna
AU - Caracciolo, Giulio
AU - Laganà, Aldo
PY - 2017/5/1
Y1 - 2017/5/1
N2 - The fast growing use of nanoparticles (NPs) in biotechnology and biomedicine raises concerns about human health and the environment. When introduced in physiological milieus, NPs adsorb biomolecules (especially proteins) forming the so-called protein corona (PC). As it is the PC that mostly interacts with biological systems, it represents a major element of the NPs’ biological identity with impact on nanotoxicology, nanosafety and targeted delivery of nanomedicines. To date, NP-protein interactions have been largely investigated in vitro, but this condition is far from mimicking the dynamic nature of physiological environments. Here we investigate the effect of shear stress on PC by exposing lipid NPs with different surface chemistry (either unmodified and PEGylated) to circulating fetal bovine serum (FBS). PC formed upon in vitro incubation was used as a reference. We demonstrate that PC is significantly influenced by exposure to dynamic flow and that changes in PC composition are dependent on both exposure time and NP's surface chemistry. Notably, alterations induced by dynamic flow affected cellular uptake of lipid NPs in both human cervical cancer (HeLa) and human breast adenocarcinoma (MCF7) cell lines.
AB - The fast growing use of nanoparticles (NPs) in biotechnology and biomedicine raises concerns about human health and the environment. When introduced in physiological milieus, NPs adsorb biomolecules (especially proteins) forming the so-called protein corona (PC). As it is the PC that mostly interacts with biological systems, it represents a major element of the NPs’ biological identity with impact on nanotoxicology, nanosafety and targeted delivery of nanomedicines. To date, NP-protein interactions have been largely investigated in vitro, but this condition is far from mimicking the dynamic nature of physiological environments. Here we investigate the effect of shear stress on PC by exposing lipid NPs with different surface chemistry (either unmodified and PEGylated) to circulating fetal bovine serum (FBS). PC formed upon in vitro incubation was used as a reference. We demonstrate that PC is significantly influenced by exposure to dynamic flow and that changes in PC composition are dependent on both exposure time and NP's surface chemistry. Notably, alterations induced by dynamic flow affected cellular uptake of lipid NPs in both human cervical cancer (HeLa) and human breast adenocarcinoma (MCF7) cell lines.
KW - Cancer cells
KW - Dynamic flow environment
KW - Nanoparticles
KW - Physiological environments
KW - Protein corona
UR - http://www.scopus.com/inward/record.url?scp=85014354605&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2017.02.037
DO - 10.1016/j.colsurfb.2017.02.037
M3 - Journal article
C2 - 28273493
AN - SCOPUS:85014354605
SN - 0927-7765
VL - 153
SP - 263
EP - 271
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -
