Increased regulatory T-cell activity and enhanced T-cell homeostatic signaling in slow progressing HIV-infected children

Julia Roider; Abigail Ngoepe; Maximilian Muenchhoff; Emily Adland; Andreas Groll; Thumbi Ndung'u; Henrik Kløverpris; Philip Goulder; Alasdair Leslie

doi:10.3389/fimmu.2019.00213

Increased regulatory T-cell activity and enhanced T-cell homeostatic signaling in slow progressing HIV-infected children

Julia Roider, Abigail Ngoepe, Maximilian Muenchhoff, Emily Adland, Andreas Groll, Thumbi Ndung'u, Henrik Kløverpris, Philip Goulder^*, Alasdair Leslie

^*Corresponding author for this work

4 Citations (Scopus)

16 Downloads (Pure)

Abstract

Pediatric slow progressors (PSP) are rare ART-naïve, HIV-infected children who maintain high CD4 T-cell counts and low immune activation despite persistently high viral loads. Using a well-defined cohort of PSP, we investigated the role of regulatory T-cells (TREG) and of IL-7 homeostatic signaling in maintaining normal-for-age CD4 counts in these individuals. Compared to children with progressive disease, PSP had greater absolute numbers of TREG, skewed toward functionally suppressive phenotypes. As with immune activation, overall T-cell proliferation was lower in PSP, but was uniquely higher in central memory TREG (CM TREG), indicating active engagement of this subset. Furthermore, PSP secreted higher levels of the immunosuppressive cytokine IL-10 than children who progressed. The frequency of suppressive TREG, CM TREG proliferation, and IL-10 production were all lower in PSP who go on to progress at a later time-point, supporting the importance of an active TREG response in preventing disease progression. In addition, we find that IL-7 homeostatic signaling is enhanced in PSP, both through preserved surface IL-7receptor (CD127) expression on central memory T-cells and increased plasma levels of soluble IL-7receptor, which enhances the bioactivity of IL-7. Combined analysis, using a LASSO modeling approach, indicates that both TREG activity and homeostatic T-cell signaling make independent contributions to the preservation of CD4 T-cells in HIV-infected children. Together, these data demonstrate that maintenance of normal-for-age CD4 counts in PSP is an active process, which requires both suppression of immune activation through functional TREG, and enhanced T-cell homeostatic signaling.

Original language	English
Article number	213
Journal	Frontiers in Immunology
Volume	10
Issue number	FEB
Number of pages	12
ISSN	1664-3224
DOIs	https://doi.org/10.3389/fimmu.2019.00213
Publication status	Published - 2019

Keywords

Homeostatic signaling
IL-10
IL-7
Immune activation (IA)
Immune regulation
Pediatric HIV-infection
Pediatric slow progression
Regulatory T cells (Treg)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Increased Regulatory T-Cell Activity and Enhanced T-Cell Homeostatic Signaling in Slow Progressing HIV-infected ChildrenFinal published version, 1.93 MBLicence: CC BY

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@article{b639cb539e8243a1b99db443ea15e2ea,

title = "Increased regulatory T-cell activity and enhanced T-cell homeostatic signaling in slow progressing HIV-infected children",

abstract = "Pediatric slow progressors (PSP) are rare ART-na{\"i}ve, HIV-infected children who maintain high CD4 T-cell counts and low immune activation despite persistently high viral loads. Using a well-defined cohort of PSP, we investigated the role of regulatory T-cells (TREG) and of IL-7 homeostatic signaling in maintaining normal-for-age CD4 counts in these individuals. Compared to children with progressive disease, PSP had greater absolute numbers of TREG, skewed toward functionally suppressive phenotypes. As with immune activation, overall T-cell proliferation was lower in PSP, but was uniquely higher in central memory TREG (CM TREG), indicating active engagement of this subset. Furthermore, PSP secreted higher levels of the immunosuppressive cytokine IL-10 than children who progressed. The frequency of suppressive TREG, CM TREG proliferation, and IL-10 production were all lower in PSP who go on to progress at a later time-point, supporting the importance of an active TREG response in preventing disease progression. In addition, we find that IL-7 homeostatic signaling is enhanced in PSP, both through preserved surface IL-7receptor (CD127) expression on central memory T-cells and increased plasma levels of soluble IL-7receptor, which enhances the bioactivity of IL-7. Combined analysis, using a LASSO modeling approach, indicates that both TREG activity and homeostatic T-cell signaling make independent contributions to the preservation of CD4 T-cells in HIV-infected children. Together, these data demonstrate that maintenance of normal-for-age CD4 counts in PSP is an active process, which requires both suppression of immune activation through functional TREG, and enhanced T-cell homeostatic signaling.",

keywords = "Homeostatic signaling, IL-10, IL-7, Immune activation (IA), Immune regulation, Pediatric HIV-infection, Pediatric slow progression, Regulatory T cells (Treg)",

author = "Julia Roider and Abigail Ngoepe and Maximilian Muenchhoff and Emily Adland and Andreas Groll and Thumbi Ndung'u and Henrik Kl{\o}verpris and Philip Goulder and Alasdair Leslie",

year = "2019",

doi = "10.3389/fimmu.2019.00213",

language = "English",

volume = "10",

journal = "Frontiers in Immunology",

issn = "1664-3224",

publisher = "Frontiers Research Foundation",

number = "FEB",

}

TY - JOUR

T1 - Increased regulatory T-cell activity and enhanced T-cell homeostatic signaling in slow progressing HIV-infected children

AU - Roider, Julia

AU - Ngoepe, Abigail

AU - Muenchhoff, Maximilian

AU - Adland, Emily

AU - Groll, Andreas

AU - Ndung'u, Thumbi

AU - Kløverpris, Henrik

AU - Goulder, Philip

AU - Leslie, Alasdair

PY - 2019

Y1 - 2019

N2 - Pediatric slow progressors (PSP) are rare ART-naïve, HIV-infected children who maintain high CD4 T-cell counts and low immune activation despite persistently high viral loads. Using a well-defined cohort of PSP, we investigated the role of regulatory T-cells (TREG) and of IL-7 homeostatic signaling in maintaining normal-for-age CD4 counts in these individuals. Compared to children with progressive disease, PSP had greater absolute numbers of TREG, skewed toward functionally suppressive phenotypes. As with immune activation, overall T-cell proliferation was lower in PSP, but was uniquely higher in central memory TREG (CM TREG), indicating active engagement of this subset. Furthermore, PSP secreted higher levels of the immunosuppressive cytokine IL-10 than children who progressed. The frequency of suppressive TREG, CM TREG proliferation, and IL-10 production were all lower in PSP who go on to progress at a later time-point, supporting the importance of an active TREG response in preventing disease progression. In addition, we find that IL-7 homeostatic signaling is enhanced in PSP, both through preserved surface IL-7receptor (CD127) expression on central memory T-cells and increased plasma levels of soluble IL-7receptor, which enhances the bioactivity of IL-7. Combined analysis, using a LASSO modeling approach, indicates that both TREG activity and homeostatic T-cell signaling make independent contributions to the preservation of CD4 T-cells in HIV-infected children. Together, these data demonstrate that maintenance of normal-for-age CD4 counts in PSP is an active process, which requires both suppression of immune activation through functional TREG, and enhanced T-cell homeostatic signaling.

AB - Pediatric slow progressors (PSP) are rare ART-naïve, HIV-infected children who maintain high CD4 T-cell counts and low immune activation despite persistently high viral loads. Using a well-defined cohort of PSP, we investigated the role of regulatory T-cells (TREG) and of IL-7 homeostatic signaling in maintaining normal-for-age CD4 counts in these individuals. Compared to children with progressive disease, PSP had greater absolute numbers of TREG, skewed toward functionally suppressive phenotypes. As with immune activation, overall T-cell proliferation was lower in PSP, but was uniquely higher in central memory TREG (CM TREG), indicating active engagement of this subset. Furthermore, PSP secreted higher levels of the immunosuppressive cytokine IL-10 than children who progressed. The frequency of suppressive TREG, CM TREG proliferation, and IL-10 production were all lower in PSP who go on to progress at a later time-point, supporting the importance of an active TREG response in preventing disease progression. In addition, we find that IL-7 homeostatic signaling is enhanced in PSP, both through preserved surface IL-7receptor (CD127) expression on central memory T-cells and increased plasma levels of soluble IL-7receptor, which enhances the bioactivity of IL-7. Combined analysis, using a LASSO modeling approach, indicates that both TREG activity and homeostatic T-cell signaling make independent contributions to the preservation of CD4 T-cells in HIV-infected children. Together, these data demonstrate that maintenance of normal-for-age CD4 counts in PSP is an active process, which requires both suppression of immune activation through functional TREG, and enhanced T-cell homeostatic signaling.

KW - Homeostatic signaling

KW - IL-10

KW - IL-7

KW - Immune activation (IA)

KW - Immune regulation

KW - Pediatric HIV-infection

KW - Pediatric slow progression

KW - Regulatory T cells (Treg)

U2 - 10.3389/fimmu.2019.00213

DO - 10.3389/fimmu.2019.00213

M3 - Journal article

C2 - 30809229

AN - SCOPUS:85065912889

SN - 1664-3224

VL - 10

JO - Frontiers in Immunology

JF - Frontiers in Immunology

IS - FEB

M1 - 213

ER -

Increased regulatory T-cell activity and enhanced T-cell homeostatic signaling in slow progressing HIV-infected children

Abstract

Keywords

UN SDGs

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