Increased cardiac BNP expression associated with myocardial ischemia

TY - JOUR

T1 - Increased cardiac BNP expression associated with myocardial ischemia

AU - Goetze, J P

AU - Christoffersen, Christina

AU - Perko, M

AU - Arendrup, H

AU - Rehfeld, J F

AU - Kastrup, J

AU - Nielsen, L B

PY - 2003/6

Y1 - 2003/6

N2 - Congestive heart failure is accompanied by increased cardiac brain natriuretic peptide (BNP) gene expression with elevated plasma concentrations of BNP and its precursor, proBNP. We investigated if myocardial ischemia in the absence of overt heart failure may be another mechanism for increased myocardial BNP expression. The BNP expression was examined in hypoxic myocardium of patients undergoing coronary bypass grafting surgery, in patients with coronary artery disease and normal left ventricular function undergoing percutaneous transluminal intervention therapy, and in heart failure patients without coronary artery disease. BNP mRNA was quantified by real-time PCR, and plasma BNP and proBNP concentrations were measured with radioimmunoassays. Quantitative analysis of BNP mRNA in atrial and ventricular biopsies from coronary bypass grafting patients revealed close associations of plasma BNP and proBNP concentrations to ventricular, but not atrial, BNP mRNA levels. Plasma BNP and proBNP concentrations were markedly increased in patients with coronary artery disease but without concomitant left ventricular dysfunction. These results are compatible with the notion that myocardial ischemia, even in the absence of left ventricular dysfunction, augments cardiac BNP gene expression and increases plasma BNP and proBNP concentrations. Thus, elevated BNP and proBNP concentrations do not necessarily reflect heart failure but may also result from cardiac ischemia.

AB - Congestive heart failure is accompanied by increased cardiac brain natriuretic peptide (BNP) gene expression with elevated plasma concentrations of BNP and its precursor, proBNP. We investigated if myocardial ischemia in the absence of overt heart failure may be another mechanism for increased myocardial BNP expression. The BNP expression was examined in hypoxic myocardium of patients undergoing coronary bypass grafting surgery, in patients with coronary artery disease and normal left ventricular function undergoing percutaneous transluminal intervention therapy, and in heart failure patients without coronary artery disease. BNP mRNA was quantified by real-time PCR, and plasma BNP and proBNP concentrations were measured with radioimmunoassays. Quantitative analysis of BNP mRNA in atrial and ventricular biopsies from coronary bypass grafting patients revealed close associations of plasma BNP and proBNP concentrations to ventricular, but not atrial, BNP mRNA levels. Plasma BNP and proBNP concentrations were markedly increased in patients with coronary artery disease but without concomitant left ventricular dysfunction. These results are compatible with the notion that myocardial ischemia, even in the absence of left ventricular dysfunction, augments cardiac BNP gene expression and increases plasma BNP and proBNP concentrations. Thus, elevated BNP and proBNP concentrations do not necessarily reflect heart failure but may also result from cardiac ischemia.

KW - Angioplasty, Balloon, Coronary

KW - Coronary Artery Bypass

KW - Coronary Artery Disease

KW - Gene Expression Regulation

KW - Heart Atria

KW - Heart Ventricles

KW - Humans

KW - Models, Cardiovascular

KW - Myocardial Ischemia

KW - Myocardium

KW - Natriuretic Peptide, Brain

KW - Nerve Tissue Proteins

KW - Peptide Fragments

KW - RNA, Messenger

U2 - 10.1096/fj.02-0796fje

DO - 10.1096/fj.02-0796fje

M3 - Journal article

C2 - 12709407

SN - 0892-6638

VL - 17

SP - 1105

EP - 1107

JO - F A S E B Journal

JF - F A S E B Journal

IS - 9

ER -