Increased capillary density in skeletal muscle is not associated with impaired insulin sensitivity induced by bed rest in healthy young men

TY - JOUR

T1 - Increased capillary density in skeletal muscle is not associated with impaired insulin sensitivity induced by bed rest in healthy young men

AU - Montero, David

AU - Oberholzer, Laura

AU - Haider, Thomas

AU - Andersen, Andreas Breenfeldt

AU - Dandanell, Sune

AU - Meinild-Lundby, Anne-Kristine

AU - Maconochie, Hannah

AU - Lundby, Carsten

N1 - CURIS 2018 NEXS 405

PY - 2018

Y1 - 2018

N2 - Physical inactivity alters glucose homeostasis in skeletal muscle, potentially developing into overt metabolic disease. The present study sought to investigate the role of skeletal muscle capillarization in glucose tolerance and insulin sensitivity (IS) using a classic human model of physical inactivity. Thirteen healthy males (age = 23 ± 2 years) underwent 4 days of full-time supervised and diet-controlled bed rest. Oral glucose tolerance test, indices of IS (quantitative insulin sensitivity check index (QUICKI), Matsuda index), as well as skeletal muscle biopsies with measurement of fiber type distribution, fiber cross-sectional area (FCSA), capillary-to-fiber ratio (C/F ratio), and capillary density (CD) were assessed prior to and after bed rest. Body weight and composition were unaltered by bed rest. Fasting glucose/insulin ratio (G 0 /I 0 ratio) (−25%, P = 0.016), QUICKI (−7%, P = 0.023), and Matsuda index (−24%, P = 0.003) diminished with bed rest. Skeletal muscle FCSA decreased (−737.4 ± 763.2 μm −2 (−12%), P = 0.005) while C/F ratio was preserved, resulting in augmented CD (+93.9 ± 91.5 capillaries·mm −2 (+37%), P = 0.003) with bed rest. No association was detected between changes in skeletal muscle variables and metabolic outcomes. Independently of bed rest-induced effects, a positive linear relationship was detected between C/F ratio and G 0 /I 0 ratio (β = 17.09, P = 0.021). In conclusion, impaired glucose homeostasis with bed rest is not prevented nor associated with enhanced skeletal muscle capillarization in healthy individuals.

AB - Physical inactivity alters glucose homeostasis in skeletal muscle, potentially developing into overt metabolic disease. The present study sought to investigate the role of skeletal muscle capillarization in glucose tolerance and insulin sensitivity (IS) using a classic human model of physical inactivity. Thirteen healthy males (age = 23 ± 2 years) underwent 4 days of full-time supervised and diet-controlled bed rest. Oral glucose tolerance test, indices of IS (quantitative insulin sensitivity check index (QUICKI), Matsuda index), as well as skeletal muscle biopsies with measurement of fiber type distribution, fiber cross-sectional area (FCSA), capillary-to-fiber ratio (C/F ratio), and capillary density (CD) were assessed prior to and after bed rest. Body weight and composition were unaltered by bed rest. Fasting glucose/insulin ratio (G 0 /I 0 ratio) (−25%, P = 0.016), QUICKI (−7%, P = 0.023), and Matsuda index (−24%, P = 0.003) diminished with bed rest. Skeletal muscle FCSA decreased (−737.4 ± 763.2 μm −2 (−12%), P = 0.005) while C/F ratio was preserved, resulting in augmented CD (+93.9 ± 91.5 capillaries·mm −2 (+37%), P = 0.003) with bed rest. No association was detected between changes in skeletal muscle variables and metabolic outcomes. Independently of bed rest-induced effects, a positive linear relationship was detected between C/F ratio and G 0 /I 0 ratio (β = 17.09, P = 0.021). In conclusion, impaired glucose homeostasis with bed rest is not prevented nor associated with enhanced skeletal muscle capillarization in healthy individuals.

KW - Faculty of Science

KW - Glucose tolerance

KW - Insulin resistance

KW - Skeletal muscle capillarization

KW - Bed rest

U2 - 10.1139/apnm-2018-0195

DO - 10.1139/apnm-2018-0195

M3 - Journal article

C2 - 29920224

SN - 1715-5312

VL - 43

SP - 1334

EP - 1340

JO - Applied Physiology, Nutrition and Metabolism

JF - Applied Physiology, Nutrition and Metabolism

IS - 12

ER -