@inbook{0ae21ad26dd640768725904dd3a089e6,
title = "In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection",
abstract = "CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver {"}naked{"} plasmid DNA to 5-40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.",
keywords = "Animals, CRISPR-Cas Systems/genetics, Gene Editing, Hepatocytes/metabolism, Liver/metabolism, Mice, Plasmids/genetics, RNA, Guide/genetics",
author = "Francesco Niola and Frederik Dagn{\ae}s-Hansen and Morten Fr{\"o}din",
year = "2019",
doi = "10.1007/978-1-4939-9170-9_20",
language = "English",
isbn = "978-1-4939-9169-3",
volume = "1961",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "329--341",
editor = "Yonglun Luo",
booktitle = "CRISPR Gene Editing",
address = "United States",
}