In vitro characterization of the Ac locus in white clover (Trifolium repens L.)

David B. Collinge; M. A. Hughes

doi:10.1016/0003-9861(82)90318-6

In vitro characterization of the Ac locus in white clover (Trifolium repens L.)

David B. Collinge^*, M. A. Hughes

^*Corresponding author for this work

Microbial Ecology and Biotechnology

19 Citations (Scopus)

Abstract

Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.

Original language	English
Journal	Archives of Biochemistry and Biophysics
Volume	218
Issue number	1
Pages (from-to)	38-45
Number of pages	8
ISSN	0003-9861
DOIs	https://doi.org/10.1016/0003-9861(82)90318-6
Publication status	Published - 1 Oct 1982

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Collinge, D. B., & Hughes, M. A. (1982). In vitro characterization of the Ac locus in white clover (Trifolium repens L.). Archives of Biochemistry and Biophysics, 218(1), 38-45. https://doi.org/10.1016/0003-9861(82)90318-6

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abstract = "Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.",

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N2 - Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.

AB - Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.

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In vitro characterization of the Ac locus in white clover (Trifolium repens L.)

Abstract

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