TY - JOUR
T1 - In vitro characterization of the Ac locus in white clover (Trifolium repens L.)
AU - Collinge, David B.
AU - Hughes, M. A.
PY - 1982/10/1
Y1 - 1982/10/1
N2 - Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.
AB - Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.
UR - http://www.scopus.com/inward/record.url?scp=0020198658&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(82)90318-6
DO - 10.1016/0003-9861(82)90318-6
M3 - Journal article
C2 - 7149740
AN - SCOPUS:0020198658
SN - 0003-9861
VL - 218
SP - 38
EP - 45
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -