In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

Jakob Toudahl Nielsen; Natalia V. Kulminskaya; Morten Bjerring; Juha M Linnanto; Margus Rätsep; Marie Østergaard Pedersen; Petar H. Lambrev; Márta Dorogi; Győző Garab; Karen Thomsen; Caroline Jegerschöld; Niels-Ulrik Frigaard; Martin Lindahl; Niels Chr. Nielsen

doi:10.1038/ncomms12454

In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

Jakob Toudahl Nielsen, Natalia V. Kulminskaya, Morten Bjerring, Juha M Linnanto, Margus Rätsep, Marie Østergaard Pedersen, Petar H. Lambrev, Márta Dorogi, Győző Garab, Karen Thomsen, Caroline Jegerschöld, Niels-Ulrik Frigaard, Martin Lindahl, Niels Chr. Nielsen

Marine Biology

30 Citations (Scopus)

400 Downloads (Pure)

Abstract

Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

Original language	English
Article number	12454
Journal	Nature Communications
Volume	7
Number of pages	12
ISSN	2041-1723
DOIs	https://doi.org/10.1038/ncomms12454
Publication status	Published - 18 Aug 2016

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In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidumFinal published version, 4.44 MBLicence: CC BY

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Nielsen, J. T., Kulminskaya, N. V., Bjerring, M., Linnanto, J. M., Rätsep, M., Pedersen, M. Ø., Lambrev, P. H., Dorogi, M., Garab, G., Thomsen, K., Jegerschöld, C., Frigaard, N-U., Lindahl, M., & Nielsen, N. C. (2016). In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum. Nature Communications, 7, [12454]. https://doi.org/10.1038/ncomms12454

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abstract = "Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.",

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T1 - In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

AU - Nielsen, Jakob Toudahl

AU - Kulminskaya, Natalia V.

AU - Bjerring, Morten

AU - Linnanto, Juha M

AU - Rätsep, Margus

AU - Pedersen, Marie Østergaard

AU - Lambrev, Petar H.

AU - Dorogi, Márta

AU - Garab, Győző

AU - Thomsen, Karen

AU - Jegerschöld, Caroline

AU - Frigaard, Niels-Ulrik

AU - Lindahl, Martin

AU - Nielsen, Niels Chr.

PY - 2016/8/18

Y1 - 2016/8/18

N2 - Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

AB - Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

KW - Journal Article

U2 - 10.1038/ncomms12454

DO - 10.1038/ncomms12454

M3 - Journal article

C2 - 27534696

SN - 2041-1723

VL - 7

JO - Nature Communications

JF - Nature Communications

M1 - 12454

ER -

In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

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