In silico assessment of virulence factors in strains of streptococcus oralis and Streptococcus mitis isolated from patients with infective endocarditis

Louise H. Rasmussen; Katrine Højholt; Rimtas Dargis; Jens Jørgen Christensen; Ole Skovgaard; Ulrik S. Justesen; Flemming S. Rosenvinge; Claus Moser; Oksana Lukjancenko; Simon Rasmussen; Xiaohui C. Nielsen

doi:10.1099/jmm.0.000573

In silico assessment of virulence factors in strains of streptococcus oralis and Streptococcus mitis isolated from patients with infective endocarditis

Louise H. Rasmussen, Katrine Højholt, Rimtas Dargis, Jens Jørgen Christensen, Ole Skovgaard, Ulrik S. Justesen, Flemming S. Rosenvinge, Claus Moser, Oksana Lukjancenko, Simon Rasmussen, Xiaohui C. Nielsen^*

^*Corresponding author for this work

Department of Clinical Medicine

12 Citations (Scopus)

Abstract

Purpose. Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though S. oralis and S. mitis are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species Streptococcus tigurinus and Streptococcus dentisani into the species S. oralis as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of S. oralis (subsp. oralis, subsp. tigurinus and subsp. dentisani) and S. mitis. Methodology. Forty clinical S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) and S. mitis genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors. Results/Key findings. Three homologues of pavA, psaA and lmb, encoding adhesion proteins, were present in all strains. Seven homologues of nanA, nanB, ply, lytA, lytB, lytC and iga, of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few S. oralis subspecies specific differences were observed. iga homologues were identified in S. oralis subsp. oralis, whereas lytA homologues were identified in S. oralis subsp. oralis and subsp. tigurinus. Conclusion. Differences in the presence of virulence factors among the three S. oralis subspecies were observed. The virulence gene profiles of the 40 S. mitis and S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) contribute with important new knowledge regarding these species and new subspecies.

Original language	English
Journal	Journal of Medical Microbiology
Volume	66
Pages (from-to)	1316-1323
Number of pages	8
ISSN	0022-2615
DOIs	https://doi.org/10.1099/jmm.0.000573
Publication status	Published - 1 Sept 2017

Keywords

Comparative genomics
Infective endocarditis
Streptococcus mitis
Streptococcus oralis
Virulence factors

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10.1099/jmm.0.000573

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Rasmussen, L. H., Højholt, K., Dargis, R., Christensen, J. J., Skovgaard, O., Justesen, U. S., Rosenvinge, F. S., Moser, C., Lukjancenko, O., Rasmussen, S., & Nielsen, X. C. (2017). In silico assessment of virulence factors in strains of streptococcus oralis and Streptococcus mitis isolated from patients with infective endocarditis. Journal of Medical Microbiology, 66, 1316-1323. https://doi.org/10.1099/jmm.0.000573

In silico assessment of virulence factors in strains of streptococcus oralis and Streptococcus mitis isolated from patients with infective endocarditis. / Rasmussen, Louise H.; Højholt, Katrine; Dargis, Rimtas et al.
In: Journal of Medical Microbiology, Vol. 66, 01.09.2017, p. 1316-1323.

Research output: Contribution to journal › Journal article › Research › peer-review

Rasmussen, LH, Højholt, K, Dargis, R, Christensen, JJ, Skovgaard, O, Justesen, US, Rosenvinge, FS, Moser, C, Lukjancenko, O, Rasmussen, S & Nielsen, XC 2017, 'In silico assessment of virulence factors in strains of streptococcus oralis and Streptococcus mitis isolated from patients with infective endocarditis', Journal of Medical Microbiology, vol. 66, pp. 1316-1323. https://doi.org/10.1099/jmm.0.000573

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title = "In silico assessment of virulence factors in strains of streptococcus oralis and Streptococcus mitis isolated from patients with infective endocarditis",

abstract = "Purpose. Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though S. oralis and S. mitis are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species Streptococcus tigurinus and Streptococcus dentisani into the species S. oralis as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of S. oralis (subsp. oralis, subsp. tigurinus and subsp. dentisani) and S. mitis. Methodology. Forty clinical S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) and S. mitis genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors. Results/Key findings. Three homologues of pavA, psaA and lmb, encoding adhesion proteins, were present in all strains. Seven homologues of nanA, nanB, ply, lytA, lytB, lytC and iga, of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few S. oralis subspecies specific differences were observed. iga homologues were identified in S. oralis subsp. oralis, whereas lytA homologues were identified in S. oralis subsp. oralis and subsp. tigurinus. Conclusion. Differences in the presence of virulence factors among the three S. oralis subspecies were observed. The virulence gene profiles of the 40 S. mitis and S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) contribute with important new knowledge regarding these species and new subspecies.",

keywords = "Comparative genomics, Infective endocarditis, Streptococcus mitis, Streptococcus oralis, Virulence factors",

author = "Rasmussen, {Louise H.} and Katrine H{\o}jholt and Rimtas Dargis and Christensen, {Jens J{\o}rgen} and Ole Skovgaard and Justesen, {Ulrik S.} and Rosenvinge, {Flemming S.} and Claus Moser and Oksana Lukjancenko and Simon Rasmussen and Nielsen, {Xiaohui C.}",

year = "2017",

month = sep,

day = "1",

doi = "10.1099/jmm.0.000573",

language = "English",

volume = "66",

pages = "1316--1323",

journal = "Journal of Medical Microbiology",

issn = "0022-2615",

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TY - JOUR

T1 - In silico assessment of virulence factors in strains of streptococcus oralis and Streptococcus mitis isolated from patients with infective endocarditis

AU - Rasmussen, Louise H.

AU - Højholt, Katrine

AU - Dargis, Rimtas

AU - Christensen, Jens Jørgen

AU - Skovgaard, Ole

AU - Justesen, Ulrik S.

AU - Rosenvinge, Flemming S.

AU - Moser, Claus

AU - Lukjancenko, Oksana

AU - Rasmussen, Simon

AU - Nielsen, Xiaohui C.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Purpose. Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though S. oralis and S. mitis are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species Streptococcus tigurinus and Streptococcus dentisani into the species S. oralis as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of S. oralis (subsp. oralis, subsp. tigurinus and subsp. dentisani) and S. mitis. Methodology. Forty clinical S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) and S. mitis genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors. Results/Key findings. Three homologues of pavA, psaA and lmb, encoding adhesion proteins, were present in all strains. Seven homologues of nanA, nanB, ply, lytA, lytB, lytC and iga, of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few S. oralis subspecies specific differences were observed. iga homologues were identified in S. oralis subsp. oralis, whereas lytA homologues were identified in S. oralis subsp. oralis and subsp. tigurinus. Conclusion. Differences in the presence of virulence factors among the three S. oralis subspecies were observed. The virulence gene profiles of the 40 S. mitis and S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) contribute with important new knowledge regarding these species and new subspecies.

AB - Purpose. Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in the human oral cavity. Even though S. oralis and S. mitis are oral commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of the Mitis group has embedded the species Streptococcus tigurinus and Streptococcus dentisani into the species S. oralis as subspecies. In this study, the distribution of virulence factors that contribute to bacterial immune evasion, colonization and adhesion was assessed in clinical strains of S. oralis (subsp. oralis, subsp. tigurinus and subsp. dentisani) and S. mitis. Methodology. Forty clinical S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) and S. mitis genomes were annotated with the pipeline PanFunPro and aligned against the VFDB database for assessment of virulence factors. Results/Key findings. Three homologues of pavA, psaA and lmb, encoding adhesion proteins, were present in all strains. Seven homologues of nanA, nanB, ply, lytA, lytB, lytC and iga, of importance regarding survival in blood and modulation of the human immune system, were variously present in the genomes. Few S. oralis subspecies specific differences were observed. iga homologues were identified in S. oralis subsp. oralis, whereas lytA homologues were identified in S. oralis subsp. oralis and subsp. tigurinus. Conclusion. Differences in the presence of virulence factors among the three S. oralis subspecies were observed. The virulence gene profiles of the 40 S. mitis and S. oralis (subsp. oralis, subsp. dentisani and subsp. tigurinus) contribute with important new knowledge regarding these species and new subspecies.

KW - Comparative genomics

KW - Infective endocarditis

KW - Streptococcus mitis

KW - Streptococcus oralis

KW - Virulence factors

U2 - 10.1099/jmm.0.000573

DO - 10.1099/jmm.0.000573

M3 - Journal article

C2 - 28874232

AN - SCOPUS:85029573039

SN - 0022-2615

VL - 66

SP - 1316

EP - 1323

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

ER -

In silico assessment of virulence factors in strains of streptococcus oralis and Streptococcus mitis isolated from patients with infective endocarditis

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Keywords

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