Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit

Frederik Banch Clausen; Grethe Risum Krog; Klaus Rieneck; Morten Hanefeld Dziegiel

doi:10.1002/pd.1605

Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit

Frederik Banch Clausen, Grethe Risum Krog, Klaus Rieneck, Morten Hanefeld Dziegiel

52 Citations (Scopus)

Abstract

Prenatal diagnostic assays have been developed using free fetal DNA circulating in the maternal blood of pregnant women. Efficient DNA extraction is crucial for a robust analysis. To improve fetal DNA yield, we tested two manual extraction methods--the NucliSens Magnetic Extraction (NMAG) system and the QIAamp DSP Virus Kit (QDSP)--against our current standard method, the widely used QIAamp DNA Blood Mini Kit (QDNA).

Original language	English
Journal	Prenatal Diagnosis
Volume	27
Issue number	1
Pages (from-to)	6-10
Number of pages	5
ISSN	0197-3851
DOIs	https://doi.org/10.1002/pd.1605
Publication status	Published - Jan 2007

Keywords

DNA
Exons
Female
Fetal Blood
Fetomaternal Transfusion
Glyceraldehyde-3-Phosphate Dehydrogenases
Humans
Magnetics
Polymerase Chain Reaction
Pregnancy
Prenatal Diagnosis
Reagent Kits, Diagnostic
Rh-Hr Blood-Group System

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Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit. / Clausen, Frederik Banch; Krog, Grethe Risum; Rieneck, Klaus et al.

In: Prenatal Diagnosis, Vol. 27, No. 1, 01.2007, p. 6-10.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Prenatal diagnostic assays have been developed using free fetal DNA circulating in the maternal blood of pregnant women. Efficient DNA extraction is crucial for a robust analysis. To improve fetal DNA yield, we tested two manual extraction methods--the NucliSens Magnetic Extraction (NMAG) system and the QIAamp DSP Virus Kit (QDSP)--against our current standard method, the widely used QIAamp DNA Blood Mini Kit (QDNA).",

keywords = "DNA, Exons, Female, Fetal Blood, Fetomaternal Transfusion, Glyceraldehyde-3-Phosphate Dehydrogenases, Humans, Magnetics, Polymerase Chain Reaction, Pregnancy, Prenatal Diagnosis, Reagent Kits, Diagnostic, Rh-Hr Blood-Group System",

author = "Clausen, {Frederik Banch} and Krog, {Grethe Risum} and Klaus Rieneck and Dziegiel, {Morten Hanefeld}",

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AU - Rieneck, Klaus

AU - Dziegiel, Morten Hanefeld

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KW - Fetomaternal Transfusion

KW - Glyceraldehyde-3-Phosphate Dehydrogenases

KW - Humans

KW - Magnetics

KW - Polymerase Chain Reaction

KW - Pregnancy

KW - Prenatal Diagnosis

KW - Reagent Kits, Diagnostic

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Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit

Abstract

Keywords

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