Improved characterization of nod factors and genetically based variation in LysM receptor domains identify amino acids expendable for nod factor recognition in Lotus spp.

Anita Søndergaard Bek; Jørgen Sauer; Mikkel Boas Thygesen; Jens Ø.  Duus; Bent O. Petersen; Søren Skou Thirup; Euan James; Knud Jørgen Jensen; Jens Stougaard; Elena Simona Radutoiu

doi:10.1094/mpmi-23-1-0058

Improved characterization of nod factors and genetically based variation in LysM receptor domains identify amino acids expendable for nod factor recognition in Lotus spp.

Anita Søndergaard Bek, Jørgen Sauer, Mikkel Boas Thygesen, Jens Ø. Duus, Bent O. Petersen, Søren Skou Thirup, Euan James, Knud Jørgen Jensen, Jens Stougaard, Elena Simona Radutoiu

48 Citations (Scopus)

Abstract

Formation of functional nodules is a complex process depending on host-microsymbiont compatibility in all developmental stages. This report uses the contrasting symbiotic phenotypes of Lotus japonicus and L. pedunculatus, inoculated with Mesorhizobium loti or the Bradyrhizobium sp. (Lotus), to investigate the role of Nod factor structure and Nod factor receptors (NFR) for rhizobial recognition, infection thread progression, and bacterial persistence within nodule cells. A key contribution was the use of 800 MHz nuclear magnetic resonance spectroscopy and ultrahigh-performance liquid chromatography coupled to quadrupole-time-offlight mass spectrometry for Nod factor analysis. The Nod factor decorations at the nonreducing end differ between Bradyrhizobium sp. (Lotus) and M. loti, and the NFR1/NFR5 extracellular regions of L. pedunculatus and L. japonicus were found to vary in amino acid composition. Genetic transformation experiments using chimeric and wild-type receptors showed that both receptor variants recognize the structurally different Nod factors but the later symbiotic phenotype remained unchanged. These results highlight the importance of additional checkpoints during nitrogen-fixing symbiosis and define several amino acids in the LysM domains as expendable for perception of the two differentially carbamoylated Nod factors.

Original language	English
Journal	Molecular Plant - Microbe Interactions
Volume	23
Issue number	1
Pages (from-to)	58-66
Number of pages	9
ISSN	0894-0282
DOIs	https://doi.org/10.1094/mpmi-23-1-0058
Publication status	Published - Jan 2010

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Bek, A. S., Sauer, J., Thygesen, M. B., Duus, J. Ø., Petersen, B. O., Thirup, S. S., James, E., Jensen, K. J., Stougaard, J., & Radutoiu, E. S. (2010). Improved characterization of nod factors and genetically based variation in LysM receptor domains identify amino acids expendable for nod factor recognition in Lotus spp. Molecular Plant - Microbe Interactions, 23(1), 58-66. https://doi.org/10.1094/mpmi-23-1-0058

Improved characterization of nod factors and genetically based variation in LysM receptor domains identify amino acids expendable for nod factor recognition in Lotus spp.. / Bek, Anita Søndergaard; Sauer, Jørgen; Thygesen, Mikkel Boas et al.

In: Molecular Plant - Microbe Interactions, Vol. 23, No. 1, 01.2010, p. 58-66.

Research output: Contribution to journal › Journal article › Research › peer-review

Bek, AS, Sauer, J, Thygesen, MB, Duus, JØ, Petersen, BO, Thirup, SS, James, E, Jensen, KJ, Stougaard, J & Radutoiu, ES 2010, 'Improved characterization of nod factors and genetically based variation in LysM receptor domains identify amino acids expendable for nod factor recognition in Lotus spp.', Molecular Plant - Microbe Interactions, vol. 23, no. 1, pp. 58-66. https://doi.org/10.1094/mpmi-23-1-0058

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title = "Improved characterization of nod factors and genetically based variation in LysM receptor domains identify amino acids expendable for nod factor recognition in Lotus spp.",

abstract = "Formation of functional nodules is a complex process depending on host-microsymbiont compatibility in all developmental stages. This report uses the contrasting symbiotic phenotypes of Lotus japonicus and L. pedunculatus, inoculated with Mesorhizobium loti or the Bradyrhizobium sp. (Lotus), to investigate the role of Nod factor structure and Nod factor receptors (NFR) for rhizobial recognition, infection thread progression, and bacterial persistence within nodule cells. A key contribution was the use of 800 MHz nuclear magnetic resonance spectroscopy and ultrahigh-performance liquid chromatography coupled to quadrupole-time-offlight mass spectrometry for Nod factor analysis. The Nod factor decorations at the nonreducing end differ between Bradyrhizobium sp. (Lotus) and M. loti, and the NFR1/NFR5 extracellular regions of L. pedunculatus and L. japonicus were found to vary in amino acid composition. Genetic transformation experiments using chimeric and wild-type receptors showed that both receptor variants recognize the structurally different Nod factors but the later symbiotic phenotype remained unchanged. These results highlight the importance of additional checkpoints during nitrogen-fixing symbiosis and define several amino acids in the LysM domains as expendable for perception of the two differentially carbamoylated Nod factors.",

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AU - Thygesen, Mikkel Boas

AU - Duus, Jens Ø.

AU - Petersen, Bent O.

AU - Thirup, Søren Skou

AU - James, Euan

AU - Jensen, Knud Jørgen

AU - Stougaard, Jens

AU - Radutoiu, Elena Simona

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N2 - Formation of functional nodules is a complex process depending on host-microsymbiont compatibility in all developmental stages. This report uses the contrasting symbiotic phenotypes of Lotus japonicus and L. pedunculatus, inoculated with Mesorhizobium loti or the Bradyrhizobium sp. (Lotus), to investigate the role of Nod factor structure and Nod factor receptors (NFR) for rhizobial recognition, infection thread progression, and bacterial persistence within nodule cells. A key contribution was the use of 800 MHz nuclear magnetic resonance spectroscopy and ultrahigh-performance liquid chromatography coupled to quadrupole-time-offlight mass spectrometry for Nod factor analysis. The Nod factor decorations at the nonreducing end differ between Bradyrhizobium sp. (Lotus) and M. loti, and the NFR1/NFR5 extracellular regions of L. pedunculatus and L. japonicus were found to vary in amino acid composition. Genetic transformation experiments using chimeric and wild-type receptors showed that both receptor variants recognize the structurally different Nod factors but the later symbiotic phenotype remained unchanged. These results highlight the importance of additional checkpoints during nitrogen-fixing symbiosis and define several amino acids in the LysM domains as expendable for perception of the two differentially carbamoylated Nod factors.

AB - Formation of functional nodules is a complex process depending on host-microsymbiont compatibility in all developmental stages. This report uses the contrasting symbiotic phenotypes of Lotus japonicus and L. pedunculatus, inoculated with Mesorhizobium loti or the Bradyrhizobium sp. (Lotus), to investigate the role of Nod factor structure and Nod factor receptors (NFR) for rhizobial recognition, infection thread progression, and bacterial persistence within nodule cells. A key contribution was the use of 800 MHz nuclear magnetic resonance spectroscopy and ultrahigh-performance liquid chromatography coupled to quadrupole-time-offlight mass spectrometry for Nod factor analysis. The Nod factor decorations at the nonreducing end differ between Bradyrhizobium sp. (Lotus) and M. loti, and the NFR1/NFR5 extracellular regions of L. pedunculatus and L. japonicus were found to vary in amino acid composition. Genetic transformation experiments using chimeric and wild-type receptors showed that both receptor variants recognize the structurally different Nod factors but the later symbiotic phenotype remained unchanged. These results highlight the importance of additional checkpoints during nitrogen-fixing symbiosis and define several amino acids in the LysM domains as expendable for perception of the two differentially carbamoylated Nod factors.

U2 - 10.1094/mpmi-23-1-0058

DO - 10.1094/mpmi-23-1-0058

M3 - Journal article

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VL - 23

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JO - Molecular Plant - Microbe Interactions

JF - Molecular Plant - Microbe Interactions

IS - 1

ER -

Improved characterization of nod factors and genetically based variation in LysM receptor domains identify amino acids expendable for nod factor recognition in Lotus spp.

Abstract

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