Impaired collagen synthesis in the rectum may be a molecular target in anastomotic leakage prophylaxis

TY - JOUR

T1 - Impaired collagen synthesis in the rectum may be a molecular target in anastomotic leakage prophylaxis

AU - Buch, Anastasia S

AU - Schjerling, Peter

AU - Kjaer, Marie

AU - Jorgensen, Lars Nannestad

AU - Krarup, Peter-Martin

AU - Ågren, Magnus S

N1 - © 2017 by the Wound Healing Society.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - The underlying molecular mechanisms for anastomotic leakage (AL) after colorectal surgery are unknown and there are no therapeutics for AL prevention. Our aim was to correlate endogenous matrix metalloproteinase (MMP) activity, collagen concentration, and collagen/MMP/cytokine mRNA levels with anatomic location in human colorectal tissue. We enrolled 22 patients in this prospective study: 7 underwent elective laparoscopic sigmoid resection and 15 underwent low anterior resection for colorectal cancer. Full-thickness intestinal tissue rings from anastomoses constructed with a circular stapler were used for the determination of the MMP activity, tissue collagen concentration and mRNA levels. COL1A1 (p = 0.017) and COL3A1 (p = 0.0013) mRNA levels were lower in rectal tissue than in colonic samples. Neither MMP activities nor collagen concentrations differed significantly between the two anatomic locations. By elucidating the factors responsible for the decreased collagen production we may identify specific molecular targets in AL prophylaxis.

AB - The underlying molecular mechanisms for anastomotic leakage (AL) after colorectal surgery are unknown and there are no therapeutics for AL prevention. Our aim was to correlate endogenous matrix metalloproteinase (MMP) activity, collagen concentration, and collagen/MMP/cytokine mRNA levels with anatomic location in human colorectal tissue. We enrolled 22 patients in this prospective study: 7 underwent elective laparoscopic sigmoid resection and 15 underwent low anterior resection for colorectal cancer. Full-thickness intestinal tissue rings from anastomoses constructed with a circular stapler were used for the determination of the MMP activity, tissue collagen concentration and mRNA levels. COL1A1 (p = 0.017) and COL3A1 (p = 0.0013) mRNA levels were lower in rectal tissue than in colonic samples. Neither MMP activities nor collagen concentrations differed significantly between the two anatomic locations. By elucidating the factors responsible for the decreased collagen production we may identify specific molecular targets in AL prophylaxis.

KW - Journal Article

U2 - 10.1111/wrr.12535

DO - 10.1111/wrr.12535

M3 - Letter

C2 - 28371122

SN - 1067-1927

VL - 25

SP - 532

EP - 535

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

IS - 3

ER -