Impact of in Vivo Ischemic Time on RNA Quality: Experiences from a Colon Cancer Biobank

TY - JOUR

T1 - Impact of in Vivo Ischemic Time on RNA Quality

T2 - Experiences from a Colon Cancer Biobank

AU - Olsen, Jesper

AU - Kirkeby, Lene T

AU - Eiholm, Susanne

AU - Jess, Per

AU - Troelsen, Jesper T

AU - Gögenür, Ismail

AU - Olsen, Jorgen

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Considerable effort has been made to improve differentiated diagnostics as well as personalized treatment for colorectal cancer patients. High-quality fresh frozen tissue is often required to investigate relevant molecular signatures in these patients. In RNA expression studies, the "RNA integrity number" is widely accepted as a reliable marker of RNA quality. Here, we investigate the feasibility of obtaining high-quality tissue from a colon cancer biobank and the impact of in vivo ischemic time and various technical and clinicopathological factors on RNA quality. Biopsies were obtained immediately following the tumor removal. The time from clamping the main arterial supply to resection and removal of the tumor was used to estimate the in vivo ischemic time. We did not observe a significant difference in RNA quality between normal tissue and tumor tissue. We observed a significant correlation between in vivo ischemic time and RNA quality in normal tissue (r = -0.24, p<0.001) but not in tumor tissue. Male gender and laparoscopic procedure were also significantly associated with lower RNA quality in normal tissue only. In tumor tissue, poor differentiation was associated with low RNA quality. In conclusion, in vivo ischemic time, surgical procedure, and gender have minor but significant effects on the quality of RNA from normal colon tissue but not tumor tissue. Poorly differentiated tumors are associated with lower RNA quality. Although its impact is low, it can still be considered to note in vivo ischemic time in colon cancer specimen procurement.

AB - Considerable effort has been made to improve differentiated diagnostics as well as personalized treatment for colorectal cancer patients. High-quality fresh frozen tissue is often required to investigate relevant molecular signatures in these patients. In RNA expression studies, the "RNA integrity number" is widely accepted as a reliable marker of RNA quality. Here, we investigate the feasibility of obtaining high-quality tissue from a colon cancer biobank and the impact of in vivo ischemic time and various technical and clinicopathological factors on RNA quality. Biopsies were obtained immediately following the tumor removal. The time from clamping the main arterial supply to resection and removal of the tumor was used to estimate the in vivo ischemic time. We did not observe a significant difference in RNA quality between normal tissue and tumor tissue. We observed a significant correlation between in vivo ischemic time and RNA quality in normal tissue (r = -0.24, p<0.001) but not in tumor tissue. Male gender and laparoscopic procedure were also significantly associated with lower RNA quality in normal tissue only. In tumor tissue, poor differentiation was associated with low RNA quality. In conclusion, in vivo ischemic time, surgical procedure, and gender have minor but significant effects on the quality of RNA from normal colon tissue but not tumor tissue. Poorly differentiated tumors are associated with lower RNA quality. Although its impact is low, it can still be considered to note in vivo ischemic time in colon cancer specimen procurement.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Biological Specimen Banks

KW - Biopsy

KW - Colon

KW - Colonic Neoplasms

KW - Female

KW - Humans

KW - Ischemia

KW - Laparoscopy

KW - Male

KW - Middle Aged

KW - Neoplasm Invasiveness

KW - RNA, Neoplasm

KW - Regression Analysis

KW - Specimen Handling

KW - Time Factors

KW - Tissue Banks

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1089/bio.2015.0009

DO - 10.1089/bio.2015.0009

M3 - Journal article

C2 - 26186671

SN - 1947-5535

VL - 13

SP - 255

EP - 262

JO - Biopreservation and Biobanking

JF - Biopreservation and Biobanking

IS - 4

ER -