Immunoproteomic characterization of outer membrane vesicles from hyper-vesiculating Actinobacillus pleuropneumoniae

Fabio Antenucci; Zofia Magnowska; Manfred Nimtz; Camille Roesch; Lothar Jänsch; Anders Miki Bojesen

doi:10.1016/j.vetmic.2019.07.001

Immunoproteomic characterization of outer membrane vesicles from hyper-vesiculating Actinobacillus pleuropneumoniae

Fabio Antenucci, Zofia Magnowska, Manfred Nimtz, Camille Roesch, Lothar Jänsch, Anders Miki Bojesen^*

^*Corresponding author for this work

1 Citation (Scopus)

Abstract

Outer membrane vesicles (OMVs) are produced and secreted virtually by every known Gram-negative bacterium. Despite their non-live nature, they share antigenic characteristics with the bacteria they originate from. This, together with their relative ease of purification, casts the OMVs as a very promising and flexible tool in both human and veterinary vaccinology. The aim of the current work was to get an insight into the antigenic pattern of OMVs from the pig pathogen Actinobacillus pleuropneumoniae in the context of vaccine development. Accordingly, we designed a protocol combining 2D Western Blotting and mass spectrometric identification to robustly characterize the antigenic protein pattern of the vesicles. Our analysis revealed that A. pleuropneumoniae OMVs carry several immunoreactive virulence factors. Some of these proteins, LpoA, OsmY and MIDG2331_02184, have never previously been documented as antigenic in A. pleuropneumoniae or other pathogenic bacteria. Additionally, we showed that despite their relative abundance, proteins such as FrpB and DegQ do not contribute to the antigenic profile of A. pleuropneumoniae OMVs.

Original language	English
Journal	Veterinary Microbiology
Volume	235
Pages (from-to)	188-194
Number of pages	7
ISSN	0378-1135
DOIs	https://doi.org/10.1016/j.vetmic.2019.07.001
Publication status	Published - 2019

Keywords

Actinobacillus pleuropneumoniae
Antigenic pattern
Immunoproteomic characterization
Outer membrane vesicles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetmic.2019.07.001

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@article{619452c9d5324e8682f9b1a4b12a7cf0,

title = "Immunoproteomic characterization of outer membrane vesicles from hyper-vesiculating Actinobacillus pleuropneumoniae",

abstract = "Outer membrane vesicles (OMVs) are produced and secreted virtually by every known Gram-negative bacterium. Despite their non-live nature, they share antigenic characteristics with the bacteria they originate from. This, together with their relative ease of purification, casts the OMVs as a very promising and flexible tool in both human and veterinary vaccinology. The aim of the current work was to get an insight into the antigenic pattern of OMVs from the pig pathogen Actinobacillus pleuropneumoniae in the context of vaccine development. Accordingly, we designed a protocol combining 2D Western Blotting and mass spectrometric identification to robustly characterize the antigenic protein pattern of the vesicles. Our analysis revealed that A. pleuropneumoniae OMVs carry several immunoreactive virulence factors. Some of these proteins, LpoA, OsmY and MIDG2331_02184, have never previously been documented as antigenic in A. pleuropneumoniae or other pathogenic bacteria. Additionally, we showed that despite their relative abundance, proteins such as FrpB and DegQ do not contribute to the antigenic profile of A. pleuropneumoniae OMVs.",

keywords = "Actinobacillus pleuropneumoniae, Antigenic pattern, Immunoproteomic characterization, Outer membrane vesicles",

author = "Fabio Antenucci and Zofia Magnowska and Manfred Nimtz and Camille Roesch and Lothar J{\"a}nsch and Bojesen, {Anders Miki}",

year = "2019",

doi = "10.1016/j.vetmic.2019.07.001",

language = "English",

volume = "235",

pages = "188--194",

journal = "Veterinary Microbiology",

issn = "0378-1135",

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TY - JOUR

T1 - Immunoproteomic characterization of outer membrane vesicles from hyper-vesiculating Actinobacillus pleuropneumoniae

AU - Antenucci, Fabio

AU - Magnowska, Zofia

AU - Nimtz, Manfred

AU - Roesch, Camille

AU - Jänsch, Lothar

AU - Bojesen, Anders Miki

PY - 2019

Y1 - 2019

N2 - Outer membrane vesicles (OMVs) are produced and secreted virtually by every known Gram-negative bacterium. Despite their non-live nature, they share antigenic characteristics with the bacteria they originate from. This, together with their relative ease of purification, casts the OMVs as a very promising and flexible tool in both human and veterinary vaccinology. The aim of the current work was to get an insight into the antigenic pattern of OMVs from the pig pathogen Actinobacillus pleuropneumoniae in the context of vaccine development. Accordingly, we designed a protocol combining 2D Western Blotting and mass spectrometric identification to robustly characterize the antigenic protein pattern of the vesicles. Our analysis revealed that A. pleuropneumoniae OMVs carry several immunoreactive virulence factors. Some of these proteins, LpoA, OsmY and MIDG2331_02184, have never previously been documented as antigenic in A. pleuropneumoniae or other pathogenic bacteria. Additionally, we showed that despite their relative abundance, proteins such as FrpB and DegQ do not contribute to the antigenic profile of A. pleuropneumoniae OMVs.

AB - Outer membrane vesicles (OMVs) are produced and secreted virtually by every known Gram-negative bacterium. Despite their non-live nature, they share antigenic characteristics with the bacteria they originate from. This, together with their relative ease of purification, casts the OMVs as a very promising and flexible tool in both human and veterinary vaccinology. The aim of the current work was to get an insight into the antigenic pattern of OMVs from the pig pathogen Actinobacillus pleuropneumoniae in the context of vaccine development. Accordingly, we designed a protocol combining 2D Western Blotting and mass spectrometric identification to robustly characterize the antigenic protein pattern of the vesicles. Our analysis revealed that A. pleuropneumoniae OMVs carry several immunoreactive virulence factors. Some of these proteins, LpoA, OsmY and MIDG2331_02184, have never previously been documented as antigenic in A. pleuropneumoniae or other pathogenic bacteria. Additionally, we showed that despite their relative abundance, proteins such as FrpB and DegQ do not contribute to the antigenic profile of A. pleuropneumoniae OMVs.

KW - Actinobacillus pleuropneumoniae

KW - Antigenic pattern

KW - Immunoproteomic characterization

KW - Outer membrane vesicles

U2 - 10.1016/j.vetmic.2019.07.001

DO - 10.1016/j.vetmic.2019.07.001

M3 - Journal article

C2 - 31383301

AN - SCOPUS:85068676939

SN - 0378-1135

VL - 235

SP - 188

EP - 194

JO - Veterinary Microbiology

JF - Veterinary Microbiology

ER -

Immunoproteomic characterization of outer membrane vesicles from hyper-vesiculating Actinobacillus pleuropneumoniae

Abstract

Keywords

UN SDGs

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