Immunogold detection of L-glutamate and D-serine in small synaptic-like microvesicles in adult hippocampal astrocytes

L H Bergersen; C Morland; L Ormel; J E Rinholm; M Larsson; J F H Wold; A T Røe; A Stranna; M Santello; D Bouvier; O P Ottersen; A Volterra; V Gundersen

doi:10.1093/cercor/bhr254

Immunogold detection of L-glutamate and D-serine in small synaptic-like microvesicles in adult hippocampal astrocytes

L H Bergersen, C Morland, L Ormel, J E Rinholm, M Larsson, J F H Wold, A T Røe, A Stranna, M Santello, D Bouvier, O P Ottersen, A Volterra, V Gundersen

74 Citations (Scopus)

Abstract

Glutamate and the N-methyl-D-aspartate receptor ligand D-serine are putative gliotransmitters. Here, we show by immunogold cytochemistry of the adult hippocampus that glutamate and D-serine accumulate in synaptic-like microvesicles (SLMVs) in the perisynaptic processes of astrocytes. The estimated concentration of fixed glutamate in the astrocytic SLMVs is comparable to that in synaptic vesicles of excitatory nerve terminals (≈ 45 and ≈ 55 mM, respectively), whereas the D-serine level is about 6 mM. The vesicles are organized in small spaced clusters located near the astrocytic plasma membrane. Endoplasmic reticulum is regularly found in close vicinity to SLMVs, suggesting that astrocytes contain functional nanodomains, where a local Ca(2+) increase can trigger release of glutamate and/or D-serine.

Original language	English
Journal	Cerebral Cortex
Volume	22
Issue number	7
Pages (from-to)	1690-7
ISSN	1047-3211
DOIs	https://doi.org/10.1093/cercor/bhr254
Publication status	Published - Jul 2012
Externally published	Yes

Keywords

Animals
Astrocytes/metabolism
Cells, Cultured
Glutamic Acid/metabolism
Gold
Hippocampus/metabolism
Immunohistochemistry/methods
Rats
Rats, Wistar
Serine/metabolism
Synaptic Vesicles/metabolism

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Bergersen, L. H., Morland, C., Ormel, L., Rinholm, J. E., Larsson, M., Wold, J. F. H., Røe, A. T., Stranna, A., Santello, M., Bouvier, D., Ottersen, O. P., Volterra, A., & Gundersen, V. (2012). Immunogold detection of L-glutamate and D-serine in small synaptic-like microvesicles in adult hippocampal astrocytes. Cerebral Cortex, 22(7), 1690-7. https://doi.org/10.1093/cercor/bhr254

Bergersen, LH, Morland, C, Ormel, L, Rinholm, JE, Larsson, M, Wold, JFH, Røe, AT, Stranna, A, Santello, M, Bouvier, D, Ottersen, OP, Volterra, A & Gundersen, V 2012, 'Immunogold detection of L-glutamate and D-serine in small synaptic-like microvesicles in adult hippocampal astrocytes', Cerebral Cortex, vol. 22, no. 7, pp. 1690-7. https://doi.org/10.1093/cercor/bhr254

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title = "Immunogold detection of L-glutamate and D-serine in small synaptic-like microvesicles in adult hippocampal astrocytes",

abstract = "Glutamate and the N-methyl-D-aspartate receptor ligand D-serine are putative gliotransmitters. Here, we show by immunogold cytochemistry of the adult hippocampus that glutamate and D-serine accumulate in synaptic-like microvesicles (SLMVs) in the perisynaptic processes of astrocytes. The estimated concentration of fixed glutamate in the astrocytic SLMVs is comparable to that in synaptic vesicles of excitatory nerve terminals (≈ 45 and ≈ 55 mM, respectively), whereas the D-serine level is about 6 mM. The vesicles are organized in small spaced clusters located near the astrocytic plasma membrane. Endoplasmic reticulum is regularly found in close vicinity to SLMVs, suggesting that astrocytes contain functional nanodomains, where a local Ca(2+) increase can trigger release of glutamate and/or D-serine.",

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doi = "10.1093/cercor/bhr254",

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AU - Bergersen, L H

AU - Morland, C

AU - Ormel, L

AU - Rinholm, J E

AU - Larsson, M

AU - Wold, J F H

AU - Røe, A T

AU - Stranna, A

AU - Santello, M

AU - Bouvier, D

AU - Ottersen, O P

AU - Volterra, A

AU - Gundersen, V

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AB - Glutamate and the N-methyl-D-aspartate receptor ligand D-serine are putative gliotransmitters. Here, we show by immunogold cytochemistry of the adult hippocampus that glutamate and D-serine accumulate in synaptic-like microvesicles (SLMVs) in the perisynaptic processes of astrocytes. The estimated concentration of fixed glutamate in the astrocytic SLMVs is comparable to that in synaptic vesicles of excitatory nerve terminals (≈ 45 and ≈ 55 mM, respectively), whereas the D-serine level is about 6 mM. The vesicles are organized in small spaced clusters located near the astrocytic plasma membrane. Endoplasmic reticulum is regularly found in close vicinity to SLMVs, suggesting that astrocytes contain functional nanodomains, where a local Ca(2+) increase can trigger release of glutamate and/or D-serine.

KW - Animals

KW - Astrocytes/metabolism

KW - Cells, Cultured

KW - Glutamic Acid/metabolism

KW - Gold

KW - Hippocampus/metabolism

KW - Immunohistochemistry/methods

KW - Rats

KW - Rats, Wistar

KW - Serine/metabolism

KW - Synaptic Vesicles/metabolism

U2 - 10.1093/cercor/bhr254

DO - 10.1093/cercor/bhr254

M3 - Journal article

C2 - 21914633

SN - 1047-3211

VL - 22

SP - 1690

EP - 1697

JO - Cerebral Cortex

JF - Cerebral Cortex

IS - 7

ER -

Immunogold detection of L-glutamate and D-serine in small synaptic-like microvesicles in adult hippocampal astrocytes

Abstract

Keywords

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