Immunoglobulin M and G antibody responses to Plasmodium falciparum glutamate-rich protein: correlation with clinical immunity in Gambian children

TY - JOUR

T1 - Immunoglobulin M and G antibody responses to Plasmodium falciparum glutamate-rich protein

T2 - correlation with clinical immunity in Gambian children

AU - Dziegiel, Morten Hanefeld

AU - Rowe, P

AU - Bennett, S

AU - Allen, S J

AU - Olerup, O

AU - Gottschau, A

AU - Borre, M

AU - Riley, E M

PY - 1993/1

Y1 - 1993/1

N2 - The aims of the present study were to describe the age-related immunoglobulin M (IgM) and IgG response to part of a 220-kDa glutamate-rich protein (GLURP) from Plasmodium falciparum and to determine possible correlations of possession of these antibodies with malaria morbidity. IgM and IgG levels were measured with a recombinant fusion protein consisting of the carboxy-terminal 783 amino acids of the GLURP. Samples for the study were obtained during a longitudinal malaria morbidity survey performed in The Gambia; cross-sectional surveys were performed at the beginning of the transmission season in May and in October. Seropositivity rates increased with age to a maximum of 77% for IgM and 95% for IgG in adults. High prevalences of seropositivity were associated with certain human leukocyte antigen class II alleles (DRw8, DR9, DR7, DR4, DQw7, and DQw2) or haplotypes. The relationship between anti-GLURP489-1271 antibodies and clinical immunity is not clear; asymptomatically infected children aged 5 to 8 years had significantly higher levels of IgG than clinically ill children of the same age, suggesting that antibodies to the carboxy-terminal part of the GLURP may contribute to immunity to P. falciparum. However, this was not significant for younger children.

AB - The aims of the present study were to describe the age-related immunoglobulin M (IgM) and IgG response to part of a 220-kDa glutamate-rich protein (GLURP) from Plasmodium falciparum and to determine possible correlations of possession of these antibodies with malaria morbidity. IgM and IgG levels were measured with a recombinant fusion protein consisting of the carboxy-terminal 783 amino acids of the GLURP. Samples for the study were obtained during a longitudinal malaria morbidity survey performed in The Gambia; cross-sectional surveys were performed at the beginning of the transmission season in May and in October. Seropositivity rates increased with age to a maximum of 77% for IgM and 95% for IgG in adults. High prevalences of seropositivity were associated with certain human leukocyte antigen class II alleles (DRw8, DR9, DR7, DR4, DQw7, and DQw2) or haplotypes. The relationship between anti-GLURP489-1271 antibodies and clinical immunity is not clear; asymptomatically infected children aged 5 to 8 years had significantly higher levels of IgG than clinically ill children of the same age, suggesting that antibodies to the carboxy-terminal part of the GLURP may contribute to immunity to P. falciparum. However, this was not significant for younger children.

KW - Adolescent

KW - Adult

KW - Age Factors

KW - Aged

KW - Aged, 80 and over

KW - Animals

KW - Antigens, Surface

KW - Child

KW - Child, Preschool

KW - Cross Reactions

KW - Cross-Sectional Studies

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Gambia

KW - HLA-DQ Antigens

KW - HLA-DR Antigens

KW - Humans

KW - Immunity

KW - Immunoglobulin G

KW - Immunoglobulin M

KW - Malaria, Falciparum

KW - Male

KW - Middle Aged

KW - Morbidity

KW - Protozoan Proteins

KW - Recombinant Fusion Proteins

KW - Sex Factors

M3 - Journal article

C2 - 8418032

SN - 0019-9567

VL - 61

SP - 103

EP - 108

JO - Infection and Immunity

JF - Infection and Immunity

IS - 1

ER -