Abstract
The glurp gene of Plasmodium falciparum F32 has been inserted into a vaccinia virus, and the recombinant virus was designated VVG4. Expression of glurp in VVG4-infected Vero cells was analyzed by immunoprecipitation and revealed a primary GLURP product of approximately 220,000 Da; GLURP was detected both intracellularly and in culture supernatants. To study the immunogenicity of vaccinia virus-expressed GLURP, mice were immunized with VVG4 and serum samples were analyzed for antibody reactivity with three polypeptides, covering almost the entire GLURP molecule; these three polypeptides were produced in recombinant form in Escherichia coli. The immune response was primarily directed against a carboxy-terminal repeat region. The mouse anti-GLURP serum recognized authentic GLURP by immunoprecipitation analysis from P. falciparum grown in vitro. These results demonstrate that vaccinia virus-expressed glurp product can induce a humoral immune response against GLURP derived from blood-stage parasites.
| Original language | English |
|---|---|
| Journal | Infection and Immunity |
| Volume | 62 |
| Issue number | 8 |
| Pages (from-to) | 3270-5 |
| Number of pages | 6 |
| ISSN | 0019-9567 |
| Publication status | Published - Aug 1994 |
| Externally published | Yes |
Keywords
- Animals
- Base Sequence
- Female
- Humans
- Malaria Vaccines/immunology
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Molecular Weight
- Plasmodium falciparum/immunology
- Protozoan Proteins/biosynthesis
- Protozoan Vaccines
- Vaccines, Synthetic/immunology
- Vaccinia virus/genetics
