Abstract
Glucagon-like-peptide 1 (GLP-1) released from the intestine is considered to be an important incretin. We have recently demonstrated that glucose-dependent insulinotropic peptide (GIP) stimulated GLP-1 secretion from canine ileal L cells in culture. To investigate further the interplay between GLP-1- and GIP-secreting cells, we set out to determine the exact location and abundance of both cell types throughout the canine intestine. Canine small intestine was subdivided into 15-20 segments and investigated by immunocytochemistry with computer-assisted imaging. The abundance of GIP-, GLP-1- and somatostatin-immunoreactive cells was determined. GIP-secreting K cells were equally distributed in duodenum and jejunum, with the GLP-1-secreting L cells concentrated in the jejunum (5% duodenum, 73% jejunum and 22% ileum). These results indicated that the middle section of the small intestine containing 69% of the K cells also contained 51% of the L cells. Double immunostaining confirmed this overlap and furthermore over 30% of the L cells in this region were found adjacent to K cells. These results suggest the existence of a paracrine interaction between the K and L cells and indicate the importance of the jejunum in the regulation of insulin release by enteric-derived incretins.
| Original language | English |
|---|---|
| Journal | Cell and Tissue Research |
| Volume | 298 |
| Issue number | 2 |
| Pages (from-to) | 287-93 |
| Number of pages | 7 |
| ISSN | 0302-766X |
| Publication status | Published - Nov 1999 |
Keywords
- Animals
- Caenorhabditis elegans Proteins
- Dogs
- Enteroendocrine Cells
- Female
- Gastric Inhibitory Polypeptide
- Immunohistochemistry
- Intestine, Small
- Killer Cells, Natural
- Male
- Membrane Glycoproteins
- Paracrine Communication
- Somatostatin
- Somatostatin-Secreting Cells
- Tissue Distribution