Immunocytochemical demonstration of polyamines in nucleoli and nuclei

Masashi Shin; Hiromichi Nakamuta; Naoko Oda-Ueda; Lars-Inge Larsson; Kunio Fujiwara

doi:10.1007/s00418-008-0400-4

Immunocytochemical demonstration of polyamines in nucleoli and nuclei

Masashi Shin, Hiromichi Nakamuta, Naoko Oda-Ueda, Lars-Inge Larsson, Kunio Fujiwara

11 Citations (Scopus)

Abstract

Although biochemical studies have shown that polyamines (PAs) occur in the nucleus, only few studies have examined the intranuclear distribution of these organic cations. By immunocytochemistry, we have previously demonstrated that PAs are located in ribosomes. We now show that PAs also ar present in both nucleoli and nuclei of a variety of cell types. Detection of nucleolar and nuclear PAs required novel pretreatment procedures involving protease and/or DNase digestion of specimens prior to immunoreaction. Double fluorescence staining confirmed the localizations. This suggests that PAs may be important to the formation of ribosomes in nucleoli, as well as adds support to biochemical studies suggesting that PAs are involved in many biological events in the nucleus. Further biochemical studies will be needed to substantiate this hypothesis.

Original language	English
Journal	Histochemistry and Cell Biology
Volume	129
Issue number	5
Pages (from-to)	659-665
Number of pages	7
ISSN	0948-6143
DOIs	https://doi.org/10.1007/s00418-008-0400-4
Publication status	Published - 2008

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title = "Immunocytochemical demonstration of polyamines in nucleoli and nuclei",

abstract = "Although biochemical studies have shown that polyamines (PAs) occur in the nucleus, only few studies have examined the intranuclear distribution of these organic cations. By immunocytochemistry, we have previously demonstrated that PAs are located in ribosomes. We now show that PAs also ar present in both nucleoli and nuclei of a variety of cell types. Detection of nucleolar and nuclear PAs required novel pretreatment procedures involving protease and/or DNase digestion of specimens prior to immunoreaction. Double fluorescence staining confirmed the localizations. This suggests that PAs may be important to the formation of ribosomes in nucleoli, as well as adds support to biochemical studies suggesting that PAs are involved in many biological events in the nucleus. Further biochemical studies will be needed to substantiate this hypothesis.",

author = "Masashi Shin and Hiromichi Nakamuta and Naoko Oda-Ueda and Lars-Inge Larsson and Kunio Fujiwara",

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T1 - Immunocytochemical demonstration of polyamines in nucleoli and nuclei

AU - Shin, Masashi

AU - Nakamuta, Hiromichi

AU - Oda-Ueda, Naoko

AU - Larsson, Lars-Inge

AU - Fujiwara, Kunio

PY - 2008

Y1 - 2008

N2 - Although biochemical studies have shown that polyamines (PAs) occur in the nucleus, only few studies have examined the intranuclear distribution of these organic cations. By immunocytochemistry, we have previously demonstrated that PAs are located in ribosomes. We now show that PAs also ar present in both nucleoli and nuclei of a variety of cell types. Detection of nucleolar and nuclear PAs required novel pretreatment procedures involving protease and/or DNase digestion of specimens prior to immunoreaction. Double fluorescence staining confirmed the localizations. This suggests that PAs may be important to the formation of ribosomes in nucleoli, as well as adds support to biochemical studies suggesting that PAs are involved in many biological events in the nucleus. Further biochemical studies will be needed to substantiate this hypothesis.

AB - Although biochemical studies have shown that polyamines (PAs) occur in the nucleus, only few studies have examined the intranuclear distribution of these organic cations. By immunocytochemistry, we have previously demonstrated that PAs are located in ribosomes. We now show that PAs also ar present in both nucleoli and nuclei of a variety of cell types. Detection of nucleolar and nuclear PAs required novel pretreatment procedures involving protease and/or DNase digestion of specimens prior to immunoreaction. Double fluorescence staining confirmed the localizations. This suggests that PAs may be important to the formation of ribosomes in nucleoli, as well as adds support to biochemical studies suggesting that PAs are involved in many biological events in the nucleus. Further biochemical studies will be needed to substantiate this hypothesis.

U2 - 10.1007/s00418-008-0400-4

DO - 10.1007/s00418-008-0400-4

M3 - Journal article

C2 - 18301910

SN - 0948-6143

VL - 129

SP - 659

EP - 665

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

IS - 5

ER -

Immunocytochemical demonstration of polyamines in nucleoli and nuclei

Abstract

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