Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine

L K Poulsen; H Nolte; I Søndergaard; P Stahl Skov

Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine

L K Poulsen, H Nolte, I Søndergaard, P Stahl Skov

Department of Clinical Medicine

Abstract

For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.

Original language	English
Journal	Agents and Actions
Volume	30
Issue number	3-4
Pages (from-to)	313-8
Number of pages	6
ISSN	0065-4299
Publication status	Published - Jun 1990

Keywords

Adsorption
Animals
Antibody Specificity
Cross Reactions
Histamine
Humans
Immunization
Immunoradiometric Assay
Immunosorbents
Iodine Radioisotopes
Rabbits
Serum Albumin
Staphylococcal Protein A
Journal Article
Research Support, Non-U.S. Gov't

Cite this

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title = "Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine",

abstract = "For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.",

keywords = "Adsorption, Animals, Antibody Specificity, Cross Reactions, Histamine, Humans, Immunization, Immunoradiometric Assay, Immunosorbents, Iodine Radioisotopes, Rabbits, Serum Albumin, Staphylococcal Protein A, Journal Article, Research Support, Non-U.S. Gov't",

author = "Poulsen, {L K} and H Nolte and I S{\o}ndergaard and {Stahl Skov}, P",

year = "1990",

month = jun,

language = "English",

volume = "30",

pages = "313--8",

journal = "Agents and Actions",

issn = "0065-4299",

publisher = "Birkhauser Verlag Basel",

number = "3-4",

}

TY - JOUR

T1 - Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine

AU - Poulsen, L K

AU - Nolte, H

AU - Søndergaard, I

AU - Stahl Skov, P

PY - 1990/6

Y1 - 1990/6

N2 - For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.

AB - For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.

KW - Adsorption

KW - Animals

KW - Antibody Specificity

KW - Cross Reactions

KW - Histamine

KW - Humans

KW - Immunization

KW - Immunoradiometric Assay

KW - Immunosorbents

KW - Iodine Radioisotopes

KW - Rabbits

KW - Serum Albumin

KW - Staphylococcal Protein A

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 2386106

SN - 0065-4299

VL - 30

SP - 313

EP - 318

JO - Agents and Actions

JF - Agents and Actions

IS - 3-4

ER -

Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine

Abstract

Keywords

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