Immune responses to band 3 neoantigens on Plasmodium falciparum-infected erythrocytes in subjects living in an area of intense malaria transmission are associated with low parasite density and high hematocrit value

B Hogh; E Petersen; I Crandall; A Gottschau; I W Sherman

Immune responses to band 3 neoantigens on Plasmodium falciparum-infected erythrocytes in subjects living in an area of intense malaria transmission are associated with low parasite density and high hematocrit value

B Hogh, E Petersen, I Crandall, A Gottschau, I W Sherman

Department of Computer Science

33 Citations (Scopus)

Abstract

During the intracellular development of the human malarial parasite, Plasmodium falciparum, cryptic regions of the erythrocyte band 3 protein are exposed. Antibodies against these band 3-related neoantigens block cytoadherence, and peptides based on amino acid sequences of putative exofacial loops of band 3 protein block the in vitro and in vivo adherence of P. falciparum-infected erythrocytes. At present, it is not known whether reactivity to these antigens is related to exposure to the malaria parasite or is correlated with protective immunity. The reactivities of plasma to peptides containing amino acid sequences of putative exofacial loops 3 and 7 of human band 3 protein were determined for children and adults living in an area of perennial malaria transmission (Liberia) and for donors who had never been exposed to malaria (Denmark). Plasma samples from children and adults living in an area of intense malaria transmission showed a much higher reactivity with the band 3 peptides than did those from nonimmune individuals. High reactivity to the loop 3 peptide (amino acids 546 to 555) was correlated with lower mean parasite density in children in the 5- to 9-year-old age group. The presence of antibodies against loop 3 and 7 peptides was not associated with a low packed erythrocyte volume (hematocrit); in fact, higher-than-average reactivities to both peptides were positively correlated with high hematocrit values, indicating that antibodies which specifically recognize the band 3-related neoantigens are not involved in hemolysis (autoimmunity).

Original language	English
Journal	Infection and Immunity
Volume	62
Issue number	10
Pages (from-to)	4362-6
Number of pages	5
ISSN	0019-9567
Publication status	Published - Oct 1994

Keywords

Adolescent
Adult
Aged
Amino Acid Sequence
Animals
Anion Exchange Protein 1, Erythrocyte/immunology
Antibodies, Protozoan/analysis
Autoimmunity
Child
Child, Preschool
Erythrocytes/parasitology
Hematocrit
Humans
Infant
Malaria, Falciparum/immunology
Middle Aged
Molecular Sequence Data
Plasmodium falciparum/immunology

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Immune responses to band 3 neoantigens on Plasmodium falciparum-infected erythrocytes in subjects living in an area of intense malaria transmission are associated with low parasite density and high hematocrit value. / Hogh, B ; Petersen, E; Crandall, I et al.
In: Infection and Immunity, Vol. 62, No. 10, 10.1994, p. 4362-6.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Immune responses to band 3 neoantigens on Plasmodium falciparum-infected erythrocytes in subjects living in an area of intense malaria transmission are associated with low parasite density and high hematocrit value",

abstract = "During the intracellular development of the human malarial parasite, Plasmodium falciparum, cryptic regions of the erythrocyte band 3 protein are exposed. Antibodies against these band 3-related neoantigens block cytoadherence, and peptides based on amino acid sequences of putative exofacial loops of band 3 protein block the in vitro and in vivo adherence of P. falciparum-infected erythrocytes. At present, it is not known whether reactivity to these antigens is related to exposure to the malaria parasite or is correlated with protective immunity. The reactivities of plasma to peptides containing amino acid sequences of putative exofacial loops 3 and 7 of human band 3 protein were determined for children and adults living in an area of perennial malaria transmission (Liberia) and for donors who had never been exposed to malaria (Denmark). Plasma samples from children and adults living in an area of intense malaria transmission showed a much higher reactivity with the band 3 peptides than did those from nonimmune individuals. High reactivity to the loop 3 peptide (amino acids 546 to 555) was correlated with lower mean parasite density in children in the 5- to 9-year-old age group. The presence of antibodies against loop 3 and 7 peptides was not associated with a low packed erythrocyte volume (hematocrit); in fact, higher-than-average reactivities to both peptides were positively correlated with high hematocrit values, indicating that antibodies which specifically recognize the band 3-related neoantigens are not involved in hemolysis (autoimmunity).",

keywords = "Adolescent, Adult, Aged, Amino Acid Sequence, Animals, Anion Exchange Protein 1, Erythrocyte/immunology, Antibodies, Protozoan/analysis, Autoimmunity, Child, Child, Preschool, Erythrocytes/parasitology, Hematocrit, Humans, Infant, Malaria, Falciparum/immunology, Middle Aged, Molecular Sequence Data, Plasmodium falciparum/immunology",

author = "B Hogh and E Petersen and I Crandall and A Gottschau and Sherman, {I W}",

year = "1994",

month = oct,

language = "English",

volume = "62",

pages = "4362--6",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Immune responses to band 3 neoantigens on Plasmodium falciparum-infected erythrocytes in subjects living in an area of intense malaria transmission are associated with low parasite density and high hematocrit value

AU - Hogh, B

AU - Petersen, E

AU - Crandall, I

AU - Gottschau, A

AU - Sherman, I W

PY - 1994/10

Y1 - 1994/10

N2 - During the intracellular development of the human malarial parasite, Plasmodium falciparum, cryptic regions of the erythrocyte band 3 protein are exposed. Antibodies against these band 3-related neoantigens block cytoadherence, and peptides based on amino acid sequences of putative exofacial loops of band 3 protein block the in vitro and in vivo adherence of P. falciparum-infected erythrocytes. At present, it is not known whether reactivity to these antigens is related to exposure to the malaria parasite or is correlated with protective immunity. The reactivities of plasma to peptides containing amino acid sequences of putative exofacial loops 3 and 7 of human band 3 protein were determined for children and adults living in an area of perennial malaria transmission (Liberia) and for donors who had never been exposed to malaria (Denmark). Plasma samples from children and adults living in an area of intense malaria transmission showed a much higher reactivity with the band 3 peptides than did those from nonimmune individuals. High reactivity to the loop 3 peptide (amino acids 546 to 555) was correlated with lower mean parasite density in children in the 5- to 9-year-old age group. The presence of antibodies against loop 3 and 7 peptides was not associated with a low packed erythrocyte volume (hematocrit); in fact, higher-than-average reactivities to both peptides were positively correlated with high hematocrit values, indicating that antibodies which specifically recognize the band 3-related neoantigens are not involved in hemolysis (autoimmunity).

AB - During the intracellular development of the human malarial parasite, Plasmodium falciparum, cryptic regions of the erythrocyte band 3 protein are exposed. Antibodies against these band 3-related neoantigens block cytoadherence, and peptides based on amino acid sequences of putative exofacial loops of band 3 protein block the in vitro and in vivo adherence of P. falciparum-infected erythrocytes. At present, it is not known whether reactivity to these antigens is related to exposure to the malaria parasite or is correlated with protective immunity. The reactivities of plasma to peptides containing amino acid sequences of putative exofacial loops 3 and 7 of human band 3 protein were determined for children and adults living in an area of perennial malaria transmission (Liberia) and for donors who had never been exposed to malaria (Denmark). Plasma samples from children and adults living in an area of intense malaria transmission showed a much higher reactivity with the band 3 peptides than did those from nonimmune individuals. High reactivity to the loop 3 peptide (amino acids 546 to 555) was correlated with lower mean parasite density in children in the 5- to 9-year-old age group. The presence of antibodies against loop 3 and 7 peptides was not associated with a low packed erythrocyte volume (hematocrit); in fact, higher-than-average reactivities to both peptides were positively correlated with high hematocrit values, indicating that antibodies which specifically recognize the band 3-related neoantigens are not involved in hemolysis (autoimmunity).

KW - Adolescent

KW - Adult

KW - Aged

KW - Amino Acid Sequence

KW - Animals

KW - Anion Exchange Protein 1, Erythrocyte/immunology

KW - Antibodies, Protozoan/analysis

KW - Autoimmunity

KW - Child

KW - Child, Preschool

KW - Erythrocytes/parasitology

KW - Hematocrit

KW - Humans

KW - Infant

KW - Malaria, Falciparum/immunology

KW - Middle Aged

KW - Molecular Sequence Data

KW - Plasmodium falciparum/immunology

M3 - Journal article

C2 - 7927696

SN - 0019-9567

VL - 62

SP - 4362

EP - 4366

JO - Infection and Immunity

JF - Infection and Immunity

IS - 10

ER -