In-vivo imaging of O2 dynamics on coral surfaces spray-painted with sensor nanoparticles

Klaus Koren; Sofie L. Jakobsen; Michael Kühl

doi:10.1016/j.snb.2016.05.147

In-vivo imaging of O₂ dynamics on coral surfaces spray-painted with sensor nanoparticles

Klaus Koren, Sofie L. Jakobsen, Michael Kühl

Marine Biology

17 Citations (Scopus)

Abstract

We describe a new method for imaging O₂ dynamics on heterogeneous biotic and abiotic surfaces using optical nanosensors. The method can overcome limitations of commonly used optodes or microsensors. To show the potential of the method, the O₂ dynamics on the surface of living corals were studied. Small coral fragments were spray-painted with O₂ sensor nanoparticles using a conventional airbrush and O₂ imaging was done with a simple SLR camera setup. Good coverage of the coral surface was achieved without significantly compromising the coral wellbeing as shown by measuring photosynthetic activity of the photosymbionts at different light levels. The method enabled analysis of spatial heterogeneities in O₂ concentration over the coral surface linked to different tissue types. Oxygenic photosynthesis and O₂ consumption could be followed at a temporal resolution of <10 s.

Original language	English
Journal	Sensors and Actuators B: Chemical
Volume	237
Pages (from-to)	1095–1101
Number of pages	7
ISSN	0925-4005
DOIs	https://doi.org/10.1016/j.snb.2016.05.147
Publication status	Published - 1 Dec 2016

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title = "In-vivo imaging of O2 dynamics on coral surfaces spray-painted with sensor nanoparticles",

abstract = "We describe a new method for imaging O2 dynamics on heterogeneous biotic and abiotic surfaces using optical nanosensors. The method can overcome limitations of commonly used optodes or microsensors. To show the potential of the method, the O2 dynamics on the surface of living corals were studied. Small coral fragments were spray-painted with O2 sensor nanoparticles using a conventional airbrush and O2 imaging was done with a simple SLR camera setup. Good coverage of the coral surface was achieved without significantly compromising the coral wellbeing as shown by measuring photosynthetic activity of the photosymbionts at different light levels. The method enabled analysis of spatial heterogeneities in O2 concentration over the coral surface linked to different tissue types. Oxygenic photosynthesis and O2 consumption could be followed at a temporal resolution of <10 s.",

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AU - Koren, Klaus

AU - Jakobsen, Sofie L.

AU - Kühl, Michael

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Y1 - 2016/12/1

N2 - We describe a new method for imaging O2 dynamics on heterogeneous biotic and abiotic surfaces using optical nanosensors. The method can overcome limitations of commonly used optodes or microsensors. To show the potential of the method, the O2 dynamics on the surface of living corals were studied. Small coral fragments were spray-painted with O2 sensor nanoparticles using a conventional airbrush and O2 imaging was done with a simple SLR camera setup. Good coverage of the coral surface was achieved without significantly compromising the coral wellbeing as shown by measuring photosynthetic activity of the photosymbionts at different light levels. The method enabled analysis of spatial heterogeneities in O2 concentration over the coral surface linked to different tissue types. Oxygenic photosynthesis and O2 consumption could be followed at a temporal resolution of <10 s.

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JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

In-vivo imaging of O2 dynamics on coral surfaces spray-painted with sensor nanoparticles

Abstract

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In-vivo imaging of O₂ dynamics on coral surfaces spray-painted with sensor nanoparticles