Identity and effects of quorum-sensing inhibitors produced by Penicillium species

Thomas Bovbjerg Rasmussen; Mette E Skindersoe; Thomas Bjarnsholt; Richard K Phipps; Kathrine Bisgaard Christensen; Peter Østrup Jensen; Jens Bo Andersen; Birgit Koch; Thomas Ostenfeld Larsen; Morten Hentzer; Leo Eberl; Niels Høiby; Michael Givskov

doi:10.1099/mic.0.27715-0

Identity and effects of quorum-sensing inhibitors produced by Penicillium species

Thomas Bovbjerg Rasmussen, Mette E Skindersoe, Thomas Bjarnsholt, Richard K Phipps, Kathrine Bisgaard Christensen, Peter Østrup Jensen, Jens Bo Andersen, Birgit Koch, Thomas Ostenfeld Larsen, Morten Hentzer, Leo Eberl, Niels Høiby, Michael Givskov

318 Citations (Scopus)

Abstract

Quorum sensing (QS) communication systems are thought to afford bacteria with a mechanism to strategically cause disease. One example is Pseudomonas aeruginosa, which infects immunocompromised individuals such as cystic fibrosis patients. The authors have previously documented that blockage of the QS systems not only attenuates Ps. aeruginosa but also renders biofilms highly susceptible to treatment with conventional antibiotics. Filamentous fungi produce a battery of secondary metabolites, some of which are already in clinical use as antimicrobial drugs. Fungi coexist with bacteria but lack active immune systems, so instead rely on chemical defence mechanisms. It was speculated that some of these secondary metabolites could interfere with bacterial QS communication. During a screening of 100 extracts from 50 Penicillium species, 33 were found to produce QS inhibitory (QSI) compounds. In two cases, patulin and penicillic acid were identified as being biologically active QSI compounds. Their effect on QS-controlled gene expression in Ps. aeruginosa was verified by DNA microarray transcriptomics. Similar to previously investigated QSI compounds, patulin was found to enhance biofilm susceptibility to tobramycin treatment. Ps. aeruginosa has developed QS-dependent mechanisms that block development of the oxidative burst in PMN neutrophils. Accordingly, when the bacteria were treated with either patulin or penicillic acid, the neutrophils became activated. In a mouse pulmonary infection model, Ps. aeruginosa was more rapidly cleared from the mice that were treated with patulin compared with the placebo group.

Original language	English
Journal	Microbiology
Volume	151
Issue number	Pt 5
Pages (from-to)	1325-40
Number of pages	15
ISSN	1350-0872
DOIs	https://doi.org/10.1099/mic.0.27715-0
Publication status	Published - 2005

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@article{e882dfd0fcee11ddb219000ea68e967b,

title = "Identity and effects of quorum-sensing inhibitors produced by Penicillium species",

abstract = "Quorum sensing (QS) communication systems are thought to afford bacteria with a mechanism to strategically cause disease. One example is Pseudomonas aeruginosa, which infects immunocompromised individuals such as cystic fibrosis patients. The authors have previously documented that blockage of the QS systems not only attenuates Ps. aeruginosa but also renders biofilms highly susceptible to treatment with conventional antibiotics. Filamentous fungi produce a battery of secondary metabolites, some of which are already in clinical use as antimicrobial drugs. Fungi coexist with bacteria but lack active immune systems, so instead rely on chemical defence mechanisms. It was speculated that some of these secondary metabolites could interfere with bacterial QS communication. During a screening of 100 extracts from 50 Penicillium species, 33 were found to produce QS inhibitory (QSI) compounds. In two cases, patulin and penicillic acid were identified as being biologically active QSI compounds. Their effect on QS-controlled gene expression in Ps. aeruginosa was verified by DNA microarray transcriptomics. Similar to previously investigated QSI compounds, patulin was found to enhance biofilm susceptibility to tobramycin treatment. Ps. aeruginosa has developed QS-dependent mechanisms that block development of the oxidative burst in PMN neutrophils. Accordingly, when the bacteria were treated with either patulin or penicillic acid, the neutrophils became activated. In a mouse pulmonary infection model, Ps. aeruginosa was more rapidly cleared from the mice that were treated with patulin compared with the placebo group.",

author = "Rasmussen, {Thomas Bovbjerg} and Skindersoe, {Mette E} and Thomas Bjarnsholt and Phipps, {Richard K} and Christensen, {Kathrine Bisgaard} and Jensen, {Peter {\O}strup} and Andersen, {Jens Bo} and Birgit Koch and Larsen, {Thomas Ostenfeld} and Morten Hentzer and Leo Eberl and Niels H{\o}iby and Michael Givskov",

note = "Keywords: Animals; Anti-Bacterial Agents; Bacterial Proteins; Biofilms; Dose-Response Relationship, Drug; Female; Gene Expression Regulation, Bacterial; Humans; Mice; Mice, Inbred BALB C; Microbial Sensitivity Tests; Neutrophils; Oligonucleotide Array Sequence Analysis; Patulin; Penicillic Acid; Penicillium; Proteome; Pseudomonas Infections; Pseudomonas aeruginosa; Signal Transduction",

year = "2005",

doi = "10.1099/mic.0.27715-0",

language = "English",

volume = "151",

pages = "1325--40",

journal = "Microbiology",

issn = "1350-0872",

publisher = "Society for General Microbiology",

number = "Pt 5",

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TY - JOUR

T1 - Identity and effects of quorum-sensing inhibitors produced by Penicillium species

AU - Rasmussen, Thomas Bovbjerg

AU - Skindersoe, Mette E

AU - Bjarnsholt, Thomas

AU - Phipps, Richard K

AU - Christensen, Kathrine Bisgaard

AU - Jensen, Peter Østrup

AU - Andersen, Jens Bo

AU - Koch, Birgit

AU - Larsen, Thomas Ostenfeld

AU - Hentzer, Morten

AU - Eberl, Leo

AU - Høiby, Niels

AU - Givskov, Michael

N1 - Keywords: Animals; Anti-Bacterial Agents; Bacterial Proteins; Biofilms; Dose-Response Relationship, Drug; Female; Gene Expression Regulation, Bacterial; Humans; Mice; Mice, Inbred BALB C; Microbial Sensitivity Tests; Neutrophils; Oligonucleotide Array Sequence Analysis; Patulin; Penicillic Acid; Penicillium; Proteome; Pseudomonas Infections; Pseudomonas aeruginosa; Signal Transduction

PY - 2005

Y1 - 2005

N2 - Quorum sensing (QS) communication systems are thought to afford bacteria with a mechanism to strategically cause disease. One example is Pseudomonas aeruginosa, which infects immunocompromised individuals such as cystic fibrosis patients. The authors have previously documented that blockage of the QS systems not only attenuates Ps. aeruginosa but also renders biofilms highly susceptible to treatment with conventional antibiotics. Filamentous fungi produce a battery of secondary metabolites, some of which are already in clinical use as antimicrobial drugs. Fungi coexist with bacteria but lack active immune systems, so instead rely on chemical defence mechanisms. It was speculated that some of these secondary metabolites could interfere with bacterial QS communication. During a screening of 100 extracts from 50 Penicillium species, 33 were found to produce QS inhibitory (QSI) compounds. In two cases, patulin and penicillic acid were identified as being biologically active QSI compounds. Their effect on QS-controlled gene expression in Ps. aeruginosa was verified by DNA microarray transcriptomics. Similar to previously investigated QSI compounds, patulin was found to enhance biofilm susceptibility to tobramycin treatment. Ps. aeruginosa has developed QS-dependent mechanisms that block development of the oxidative burst in PMN neutrophils. Accordingly, when the bacteria were treated with either patulin or penicillic acid, the neutrophils became activated. In a mouse pulmonary infection model, Ps. aeruginosa was more rapidly cleared from the mice that were treated with patulin compared with the placebo group.

AB - Quorum sensing (QS) communication systems are thought to afford bacteria with a mechanism to strategically cause disease. One example is Pseudomonas aeruginosa, which infects immunocompromised individuals such as cystic fibrosis patients. The authors have previously documented that blockage of the QS systems not only attenuates Ps. aeruginosa but also renders biofilms highly susceptible to treatment with conventional antibiotics. Filamentous fungi produce a battery of secondary metabolites, some of which are already in clinical use as antimicrobial drugs. Fungi coexist with bacteria but lack active immune systems, so instead rely on chemical defence mechanisms. It was speculated that some of these secondary metabolites could interfere with bacterial QS communication. During a screening of 100 extracts from 50 Penicillium species, 33 were found to produce QS inhibitory (QSI) compounds. In two cases, patulin and penicillic acid were identified as being biologically active QSI compounds. Their effect on QS-controlled gene expression in Ps. aeruginosa was verified by DNA microarray transcriptomics. Similar to previously investigated QSI compounds, patulin was found to enhance biofilm susceptibility to tobramycin treatment. Ps. aeruginosa has developed QS-dependent mechanisms that block development of the oxidative burst in PMN neutrophils. Accordingly, when the bacteria were treated with either patulin or penicillic acid, the neutrophils became activated. In a mouse pulmonary infection model, Ps. aeruginosa was more rapidly cleared from the mice that were treated with patulin compared with the placebo group.

U2 - 10.1099/mic.0.27715-0

DO - 10.1099/mic.0.27715-0

M3 - Journal article

C2 - 15870443

SN - 1350-0872

VL - 151

SP - 1325

EP - 1340

JO - Microbiology

JF - Microbiology

IS - Pt 5

ER -

Identity and effects of quorum-sensing inhibitors produced by Penicillium species

Abstract

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