Identification of three QTLs with influence on susceptibility to helminth infections in pigs

TY - ABST

T1 - Identification of three QTLs with influence on susceptibility to helminth infections in pigs

AU - Nejsum, Peter

AU - Thamsborg, Stig Milan

AU - Göring, H. H.

AU - Karlskov-Mortensen, Peter

AU - Archibald, A. L.

AU - Roepstorff, Allan Knud

AU - Anderson, T. J. C.

AU - Fredholm, Merete

AU - Jørgensen, Claus Bøttcher

PY - 2010

Y1 - 2010

N2 -   Intestinal helminth infections are causing health and welfare problems in both human and animal populations. A family, in which susceptibility towards Ascaris (large round worm) and Trichuris (whipworm) infections are segregating, was constructed. Our data demonstrate that genetic components are responsible for approximately 45% and 70% of the variation in Ascaris and Trichuris parasite loads, respectively. A genome scan using the Ilummina 7K SNP-chip has been performed in order to locate genomic regions controlling this susceptibility. A total of 194 pigs from 19 full-sib litters have been genotyped and 3955 informative SNPs were used to perform genotype association analysis. For Trichuris parasite load (faecal egg excretion) four SNPs in a 2 Mb region on SSC13 and 4 SNPs in a 7 Mb region on SSC11 have been identified, whereas 7 SNPs located within a 6 Mb region on SSC4 were associated with Ascaris egg excretion and worm load. The proportion of phenotypic variance accounted for by a SNP's genotype ranged from 0.08-0.14 and 0.06-0.12 for Ascaris and Trichuris, respectively. The identified QTLs will be verified in unrelated pig material and we are currently collecting samples for that purpose. Re-sequencing technology will be applied in candidate regions on pigs with deviating phenotypes, which will provide information on all genetic differences in the genomic regions.

AB -   Intestinal helminth infections are causing health and welfare problems in both human and animal populations. A family, in which susceptibility towards Ascaris (large round worm) and Trichuris (whipworm) infections are segregating, was constructed. Our data demonstrate that genetic components are responsible for approximately 45% and 70% of the variation in Ascaris and Trichuris parasite loads, respectively. A genome scan using the Ilummina 7K SNP-chip has been performed in order to locate genomic regions controlling this susceptibility. A total of 194 pigs from 19 full-sib litters have been genotyped and 3955 informative SNPs were used to perform genotype association analysis. For Trichuris parasite load (faecal egg excretion) four SNPs in a 2 Mb region on SSC13 and 4 SNPs in a 7 Mb region on SSC11 have been identified, whereas 7 SNPs located within a 6 Mb region on SSC4 were associated with Ascaris egg excretion and worm load. The proportion of phenotypic variance accounted for by a SNP's genotype ranged from 0.08-0.14 and 0.06-0.12 for Ascaris and Trichuris, respectively. The identified QTLs will be verified in unrelated pig material and we are currently collecting samples for that purpose. Re-sequencing technology will be applied in candidate regions on pigs with deviating phenotypes, which will provide information on all genetic differences in the genomic regions.

M3 - Conference abstract for conference

T2 - Joint Spring Symposium of the Danish Society for Parasitology & Danish Society for Tropical Medicine and International Health

Y2 - 19 March 2010

ER -