Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection

Hitokazu Esaki; David A Ewald; Benjamin Ungar; Mariya Rozenblit; Xiuzhong Zheng; Hui Xu; Yeriel D Estrada; Xiangyu Peng; Hiroshi Mitsui; Thomas Litman; Mayte Suárez-Fariñas; James G Krueger; Emma Guttman-Yassky

doi:10.1016/j.jaci.2014.10.037

Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection

Hitokazu Esaki, David A Ewald, Benjamin Ungar, Mariya Rozenblit, Xiuzhong Zheng, Hui Xu, Yeriel D Estrada, Xiangyu Peng, Hiroshi Mitsui, Thomas Litman, Mayte Suárez-Fariñas, James G Krueger, Emma Guttman-Yassky

112 Citations (Scopus)

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Abstract

BACKGROUND: The molecular signature of atopic dermatitis (AD) lesions is associated with TH2 and TH22 activation and epidermal alterations. However, the epidermal and dermal AD transcriptomes and their respective contributions to abnormalities in respective immune and barrier phenotypes are unknown.

OBJECTIVE: We sought to establish the genomic profile of the epidermal and dermal compartments of lesional and nonlesional AD skin compared with normal skin.

METHODS: Laser capture microdissection was performed to separate the epidermis and dermis of lesional and nonlesional skin from patients with AD and normal skin from healthy volunteers, followed by gene expression (microarrays and real-time PCR) and immunostaining studies.

RESULTS: Our study identified novel immune and barrier genes, including the IL-34 cytokine and claudins 4 and 8, and showed increased detection of key AD genes usually undetectable on arrays (ie, IL22, thymic stromal lymphopoietin [TSLP], CCL22, and CCL26). Overall, the combined epidermal and dermal transcriptomes enlarged the AD transcriptome, adding 674 upregulated and 405 downregulated differentially expressed genes between lesional and nonlesional skin to the AD transcriptome. We were also able to localize individual transcripts as primarily epidermal (defensin, beta 4A [DEFB4A]) or dermal (IL22, cytotoxic T-lymphocyte antigen 4 [CTLA4], and CCR7) and link their expressions to possible cellular sources.

CONCLUSIONS: This is the first report that establishes robust epidermal and dermal genomic signatures of lesional and nonlesional AD skin and normal skin compared with whole tissues. These data establish the utility of laser capture microdissection to separate different compartments and cellular subsets in patients with AD, allowing localization of key barrier or immune molecules and enabling detection of gene products usually not detected on arrays.

Original language	English
Journal	The Journal of allergy and clinical immunology
Volume	135
Issue number	1
Pages (from-to)	153-63
Number of pages	11
ISSN	0091-6749
DOIs	https://doi.org/10.1016/j.jaci.2014.10.037
Publication status	Published - 1 Jan 2015
Externally published	Yes

Keywords

Adult
Dermatitis, Atopic/genetics
Female
Gene Expression Profiling
Humans
Laser Capture Microdissection
Male
Middle Aged
Oligonucleotide Array Sequence Analysis
Real-Time Polymerase Chain Reaction
Skin/metabolism

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10.1016/j.jaci.2014.10.037

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Esaki, H., Ewald, D. A., Ungar, B., Rozenblit, M., Zheng, X., Xu, H., Estrada, Y. D., Peng, X., Mitsui, H., Litman, T., Suárez-Fariñas, M., Krueger, J. G., & Guttman-Yassky, E. (2015). Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection. The Journal of allergy and clinical immunology, 135(1), 153-63. https://doi.org/10.1016/j.jaci.2014.10.037

Esaki, H, Ewald, DA, Ungar, B, Rozenblit, M, Zheng, X, Xu, H, Estrada, YD, Peng, X, Mitsui, H, Litman, T, Suárez-Fariñas, M, Krueger, JG & Guttman-Yassky, E 2015, 'Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection', The Journal of allergy and clinical immunology, vol. 135, no. 1, pp. 153-63. https://doi.org/10.1016/j.jaci.2014.10.037

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title = "Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection",

abstract = "BACKGROUND: The molecular signature of atopic dermatitis (AD) lesions is associated with TH2 and TH22 activation and epidermal alterations. However, the epidermal and dermal AD transcriptomes and their respective contributions to abnormalities in respective immune and barrier phenotypes are unknown.OBJECTIVE: We sought to establish the genomic profile of the epidermal and dermal compartments of lesional and nonlesional AD skin compared with normal skin.METHODS: Laser capture microdissection was performed to separate the epidermis and dermis of lesional and nonlesional skin from patients with AD and normal skin from healthy volunteers, followed by gene expression (microarrays and real-time PCR) and immunostaining studies.RESULTS: Our study identified novel immune and barrier genes, including the IL-34 cytokine and claudins 4 and 8, and showed increased detection of key AD genes usually undetectable on arrays (ie, IL22, thymic stromal lymphopoietin [TSLP], CCL22, and CCL26). Overall, the combined epidermal and dermal transcriptomes enlarged the AD transcriptome, adding 674 upregulated and 405 downregulated differentially expressed genes between lesional and nonlesional skin to the AD transcriptome. We were also able to localize individual transcripts as primarily epidermal (defensin, beta 4A [DEFB4A]) or dermal (IL22, cytotoxic T-lymphocyte antigen 4 [CTLA4], and CCR7) and link their expressions to possible cellular sources.CONCLUSIONS: This is the first report that establishes robust epidermal and dermal genomic signatures of lesional and nonlesional AD skin and normal skin compared with whole tissues. These data establish the utility of laser capture microdissection to separate different compartments and cellular subsets in patients with AD, allowing localization of key barrier or immune molecules and enabling detection of gene products usually not detected on arrays.",

keywords = "Adult, Dermatitis, Atopic/genetics, Female, Gene Expression Profiling, Humans, Laser Capture Microdissection, Male, Middle Aged, Oligonucleotide Array Sequence Analysis, Real-Time Polymerase Chain Reaction, Skin/metabolism",

author = "Hitokazu Esaki and Ewald, {David A} and Benjamin Ungar and Mariya Rozenblit and Xiuzhong Zheng and Hui Xu and Estrada, {Yeriel D} and Xiangyu Peng and Hiroshi Mitsui and Thomas Litman and Mayte Su{\'a}rez-Fari{\~n}as and Krueger, {James G} and Emma Guttman-Yassky",

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doi = "10.1016/j.jaci.2014.10.037",

language = "English",

volume = "135",

pages = "153--63",

journal = "Journal of Allergy and Clinical Immunology",

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T1 - Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection

AU - Esaki, Hitokazu

AU - Ewald, David A

AU - Ungar, Benjamin

AU - Rozenblit, Mariya

AU - Zheng, Xiuzhong

AU - Xu, Hui

AU - Estrada, Yeriel D

AU - Peng, Xiangyu

AU - Mitsui, Hiroshi

AU - Litman, Thomas

AU - Suárez-Fariñas, Mayte

AU - Krueger, James G

AU - Guttman-Yassky, Emma

PY - 2015/1/1

Y1 - 2015/1/1

N2 - BACKGROUND: The molecular signature of atopic dermatitis (AD) lesions is associated with TH2 and TH22 activation and epidermal alterations. However, the epidermal and dermal AD transcriptomes and their respective contributions to abnormalities in respective immune and barrier phenotypes are unknown.OBJECTIVE: We sought to establish the genomic profile of the epidermal and dermal compartments of lesional and nonlesional AD skin compared with normal skin.METHODS: Laser capture microdissection was performed to separate the epidermis and dermis of lesional and nonlesional skin from patients with AD and normal skin from healthy volunteers, followed by gene expression (microarrays and real-time PCR) and immunostaining studies.RESULTS: Our study identified novel immune and barrier genes, including the IL-34 cytokine and claudins 4 and 8, and showed increased detection of key AD genes usually undetectable on arrays (ie, IL22, thymic stromal lymphopoietin [TSLP], CCL22, and CCL26). Overall, the combined epidermal and dermal transcriptomes enlarged the AD transcriptome, adding 674 upregulated and 405 downregulated differentially expressed genes between lesional and nonlesional skin to the AD transcriptome. We were also able to localize individual transcripts as primarily epidermal (defensin, beta 4A [DEFB4A]) or dermal (IL22, cytotoxic T-lymphocyte antigen 4 [CTLA4], and CCR7) and link their expressions to possible cellular sources.CONCLUSIONS: This is the first report that establishes robust epidermal and dermal genomic signatures of lesional and nonlesional AD skin and normal skin compared with whole tissues. These data establish the utility of laser capture microdissection to separate different compartments and cellular subsets in patients with AD, allowing localization of key barrier or immune molecules and enabling detection of gene products usually not detected on arrays.

AB - BACKGROUND: The molecular signature of atopic dermatitis (AD) lesions is associated with TH2 and TH22 activation and epidermal alterations. However, the epidermal and dermal AD transcriptomes and their respective contributions to abnormalities in respective immune and barrier phenotypes are unknown.OBJECTIVE: We sought to establish the genomic profile of the epidermal and dermal compartments of lesional and nonlesional AD skin compared with normal skin.METHODS: Laser capture microdissection was performed to separate the epidermis and dermis of lesional and nonlesional skin from patients with AD and normal skin from healthy volunteers, followed by gene expression (microarrays and real-time PCR) and immunostaining studies.RESULTS: Our study identified novel immune and barrier genes, including the IL-34 cytokine and claudins 4 and 8, and showed increased detection of key AD genes usually undetectable on arrays (ie, IL22, thymic stromal lymphopoietin [TSLP], CCL22, and CCL26). Overall, the combined epidermal and dermal transcriptomes enlarged the AD transcriptome, adding 674 upregulated and 405 downregulated differentially expressed genes between lesional and nonlesional skin to the AD transcriptome. We were also able to localize individual transcripts as primarily epidermal (defensin, beta 4A [DEFB4A]) or dermal (IL22, cytotoxic T-lymphocyte antigen 4 [CTLA4], and CCR7) and link their expressions to possible cellular sources.CONCLUSIONS: This is the first report that establishes robust epidermal and dermal genomic signatures of lesional and nonlesional AD skin and normal skin compared with whole tissues. These data establish the utility of laser capture microdissection to separate different compartments and cellular subsets in patients with AD, allowing localization of key barrier or immune molecules and enabling detection of gene products usually not detected on arrays.

KW - Adult

KW - Dermatitis, Atopic/genetics

KW - Female

KW - Gene Expression Profiling

KW - Humans

KW - Laser Capture Microdissection

KW - Male

KW - Middle Aged

KW - Oligonucleotide Array Sequence Analysis

KW - Real-Time Polymerase Chain Reaction

KW - Skin/metabolism

U2 - 10.1016/j.jaci.2014.10.037

DO - 10.1016/j.jaci.2014.10.037

M3 - Journal article

C2 - 25567045

SN - 0091-6749

VL - 135

SP - 153

EP - 163

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

IS - 1

ER -

Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection

Abstract

Keywords

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