Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex

Charlotte Kawecki; Olivier Bocquet; Christian E H Schmelzer; Andrea Heinz; Christian Ihling; Amandine Wahart; Béatrice Romier; Amar Bennasroune; Sébastien Blaise; Christine Terryn; Kenneth J Linton; Laurent Martiny; Laurent Duca; Pascal Maurice

doi:10.1007/s00018-018-2978-6

Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex

Charlotte Kawecki, Olivier Bocquet, Christian E H Schmelzer, Andrea Heinz, Christian Ihling, Amandine Wahart, Béatrice Romier, Amar Bennasroune, Sébastien Blaise, Christine Terryn, Kenneth J Linton, Laurent Martiny, Laurent Duca, Pascal Maurice

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Abstract

In addition to its critical role in lysosomes for catabolism of sialoglycoconjugates, NEU1 is expressed at the plasma membrane and regulates a myriad of receptors by desialylation, playing a key role in many pathophysiological processes. Here, we developed a proteomic approach dedicated to the purification and identification by LC-MS/MS of plasma membrane NEU1 interaction partners in human macrophages. Already known interaction partners were identified as well as several new candidates such as the class B scavenger receptor CD36. Interaction between NEU1 and CD36 was confirmed by complementary approaches. We showed that elastin-derived peptides (EDP) desialylate CD36 and that this effect was blocked by the V14 peptide, which blocks the interaction between bioactive EDP and the elastin receptor complex (ERC). Importantly, EDP also increased the uptake of oxidized LDL by macrophages that is blocked by both the V14 peptide and the sialidase inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA). These results demonstrate, for the first time, that binding of EDP to the ERC indirectly modulates CD36 sialylation level and regulates oxidized LDL uptake through this sialidase. These effects could contribute to the previously reported proatherogenic role of EDP and add a new dimension in the regulation of biological processes through NEU1.

Original language	English
Journal	Cellular and molecular life sciences : CMLS
Volume	76
Issue number	4
Pages (from-to)	791–807
ISSN	1420-682X
DOIs	https://doi.org/10.1007/s00018-018-2978-6
Publication status	Published - 28 Feb 2019

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Identification of CD36 as a new interaction partner of membrane NEU1Final published version, 2.11 MBLicence: CC BY

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Kawecki, C., Bocquet, O., Schmelzer, C. E. H., Heinz, A., Ihling, C., Wahart, A., Romier, B., Bennasroune, A., Blaise, S., Terryn, C., Linton, K. J., Martiny, L., Duca, L., & Maurice, P. (2019). Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex. Cellular and molecular life sciences : CMLS, 76(4), 791–807. https://doi.org/10.1007/s00018-018-2978-6

Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex. / Kawecki, Charlotte; Bocquet, Olivier; Schmelzer, Christian E H et al.
In: Cellular and molecular life sciences : CMLS, Vol. 76, No. 4, 28.02.2019, p. 791–807.

Research output: Contribution to journal › Journal article › Research › peer-review

Kawecki, C, Bocquet, O, Schmelzer, CEH, Heinz, A, Ihling, C, Wahart, A, Romier, B, Bennasroune, A, Blaise, S, Terryn, C, Linton, KJ, Martiny, L, Duca, L & Maurice, P 2019, 'Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex', Cellular and molecular life sciences : CMLS, vol. 76, no. 4, pp. 791–807. https://doi.org/10.1007/s00018-018-2978-6

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title = "Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex",

abstract = "In addition to its critical role in lysosomes for catabolism of sialoglycoconjugates, NEU1 is expressed at the plasma membrane and regulates a myriad of receptors by desialylation, playing a key role in many pathophysiological processes. Here, we developed a proteomic approach dedicated to the purification and identification by LC-MS/MS of plasma membrane NEU1 interaction partners in human macrophages. Already known interaction partners were identified as well as several new candidates such as the class B scavenger receptor CD36. Interaction between NEU1 and CD36 was confirmed by complementary approaches. We showed that elastin-derived peptides (EDP) desialylate CD36 and that this effect was blocked by the V14 peptide, which blocks the interaction between bioactive EDP and the elastin receptor complex (ERC). Importantly, EDP also increased the uptake of oxidized LDL by macrophages that is blocked by both the V14 peptide and the sialidase inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA). These results demonstrate, for the first time, that binding of EDP to the ERC indirectly modulates CD36 sialylation level and regulates oxidized LDL uptake through this sialidase. These effects could contribute to the previously reported proatherogenic role of EDP and add a new dimension in the regulation of biological processes through NEU1.",

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T1 - Identification of CD36 as a new interaction partner of membrane NEU1

T2 - potential implication in the pro-atherogenic effects of the elastin receptor complex

AU - Kawecki, Charlotte

AU - Bocquet, Olivier

AU - Schmelzer, Christian E H

AU - Heinz, Andrea

AU - Ihling, Christian

AU - Wahart, Amandine

AU - Romier, Béatrice

AU - Bennasroune, Amar

AU - Blaise, Sébastien

AU - Terryn, Christine

AU - Linton, Kenneth J

AU - Martiny, Laurent

AU - Duca, Laurent

AU - Maurice, Pascal

PY - 2019/2/28

Y1 - 2019/2/28

N2 - In addition to its critical role in lysosomes for catabolism of sialoglycoconjugates, NEU1 is expressed at the plasma membrane and regulates a myriad of receptors by desialylation, playing a key role in many pathophysiological processes. Here, we developed a proteomic approach dedicated to the purification and identification by LC-MS/MS of plasma membrane NEU1 interaction partners in human macrophages. Already known interaction partners were identified as well as several new candidates such as the class B scavenger receptor CD36. Interaction between NEU1 and CD36 was confirmed by complementary approaches. We showed that elastin-derived peptides (EDP) desialylate CD36 and that this effect was blocked by the V14 peptide, which blocks the interaction between bioactive EDP and the elastin receptor complex (ERC). Importantly, EDP also increased the uptake of oxidized LDL by macrophages that is blocked by both the V14 peptide and the sialidase inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA). These results demonstrate, for the first time, that binding of EDP to the ERC indirectly modulates CD36 sialylation level and regulates oxidized LDL uptake through this sialidase. These effects could contribute to the previously reported proatherogenic role of EDP and add a new dimension in the regulation of biological processes through NEU1.

AB - In addition to its critical role in lysosomes for catabolism of sialoglycoconjugates, NEU1 is expressed at the plasma membrane and regulates a myriad of receptors by desialylation, playing a key role in many pathophysiological processes. Here, we developed a proteomic approach dedicated to the purification and identification by LC-MS/MS of plasma membrane NEU1 interaction partners in human macrophages. Already known interaction partners were identified as well as several new candidates such as the class B scavenger receptor CD36. Interaction between NEU1 and CD36 was confirmed by complementary approaches. We showed that elastin-derived peptides (EDP) desialylate CD36 and that this effect was blocked by the V14 peptide, which blocks the interaction between bioactive EDP and the elastin receptor complex (ERC). Importantly, EDP also increased the uptake of oxidized LDL by macrophages that is blocked by both the V14 peptide and the sialidase inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA). These results demonstrate, for the first time, that binding of EDP to the ERC indirectly modulates CD36 sialylation level and regulates oxidized LDL uptake through this sialidase. These effects could contribute to the previously reported proatherogenic role of EDP and add a new dimension in the regulation of biological processes through NEU1.

U2 - 10.1007/s00018-018-2978-6

DO - 10.1007/s00018-018-2978-6

M3 - Journal article

C2 - 30498996

SN - 1420-682X

VL - 76

SP - 791

EP - 807

JO - Cellular and molecular life sciences : CMLS

JF - Cellular and molecular life sciences : CMLS

IS - 4

ER -

Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex

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