Identification of a novel human tRNA(Ser(CGA)) functional in murine leukemia virus replication.

TY - JOUR

T1 - Identification of a novel human tRNA(Ser(CGA)) functional in murine leukemia virus replication.

AU - Lund, Anders Henrik

AU - Schmitz, A

AU - Pedersen, F S

AU - Duch, M

N1 - Keywords: Animals; Base Sequence; Cells, Cultured; Chromosome Mapping; Chromosomes, Human, Pair 6; DNA; Genome, Human; Haplorhini; Hela Cells; Humans; Leukemia Virus, Murine; Mice; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Transfer, Ser; Virus Replication

PY - 2000

Y1 - 2000

N2 - We have identified a human tRNA(Ser) isoacceptor matching the UCG codon. The tRNA was discovered via its ability to act in reverse transcription of a murine leukemia virus vector containing a complementary tRNA primer binding site (Lund et al., Nucleic Acids Res., 28 (2000) 791-799). The tRNA(Ser(CGA)) was detected in cell lines of human, monkey and mouse origin. The UCG codon is the most rarely used codon in human genes. The cloned human tRNA(Ser(CGA)) gene encodes an 85 nucleotide, intron-less tRNA, contains a consensus split intragenic promoter and is located at region p21.3-22.2 on chromosome 6. The integrity and functionality of the cloned tRNA(Ser(CGA)) gene was verified by in vitro transcription analysis in HeLa nuclear extracts.

AB - We have identified a human tRNA(Ser) isoacceptor matching the UCG codon. The tRNA was discovered via its ability to act in reverse transcription of a murine leukemia virus vector containing a complementary tRNA primer binding site (Lund et al., Nucleic Acids Res., 28 (2000) 791-799). The tRNA(Ser(CGA)) was detected in cell lines of human, monkey and mouse origin. The UCG codon is the most rarely used codon in human genes. The cloned human tRNA(Ser(CGA)) gene encodes an 85 nucleotide, intron-less tRNA, contains a consensus split intragenic promoter and is located at region p21.3-22.2 on chromosome 6. The integrity and functionality of the cloned tRNA(Ser(CGA)) gene was verified by in vitro transcription analysis in HeLa nuclear extracts.

M3 - Journal article

C2 - 11004500

SN - 0304-4165

VL - 1492

SP - 264

EP - 268

JO - B B A - General Subjects

JF - B B A - General Subjects

IS - 1

ER -