Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes

TY - JOUR

T1 - Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes

AU - Sørensen, Iben Schildt

AU - Dandanell, Gert

PY - 1997

Y1 - 1997

N2 - From a genomic library of Sulfolobus solfataricus DSM1617 we have isolated and identified the purEK locus. Two open reading frames are identified as homologs of the purE and purK purine biosynthetic genes in Escherichia coli. The C-terminus of purE overlaps with the N-terminus of purK. When either of the genes is expressed from an E. coli promoter they can complement the corresponding purE and purK mutations in E. coli. PurE seems to be more closely related to eubacteria than to other archaea and to eukaryotes. Also the purK gene, which has not yet been found in other archaea, is more closely related to eubacteria than to eukaryotes.

AB - From a genomic library of Sulfolobus solfataricus DSM1617 we have isolated and identified the purEK locus. Two open reading frames are identified as homologs of the purE and purK purine biosynthetic genes in Escherichia coli. The C-terminus of purE overlaps with the N-terminus of purK. When either of the genes is expressed from an E. coli promoter they can complement the corresponding purE and purK mutations in E. coli. PurE seems to be more closely related to eubacteria than to other archaea and to eukaryotes. Also the purK gene, which has not yet been found in other archaea, is more closely related to eubacteria than to eukaryotes.

M3 - Journal article

SN - 0378-1097

VL - 154

SP - 173

EP - 180

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -