Agrobacterium rhizogenes mediated transformation of Rhodiola sp. - an approach to enhance the level of bioactive compounds

TY - GEN

T1 - Agrobacterium rhizogenes mediated transformation of Rhodiola sp. - an approach to enhance the level of bioactive compounds

AU - Himmelboe, Martin

AU - Lauridsen, Uffe Bjerre

AU - Hegelund, Josefine Nymark

AU - Müller, Renate

AU - Lütken, Henrik Vlk

PY - 2015

Y1 - 2015

N2 - Rhodiola rosea, commonly known as roseroot, has since ancient times been used against depression and for improving mental abilities because of its bioactive compounds. Due to excessive exploitation, the natural populations have been declining. Natural transformation with root-loci (rol)-genes from wildtype Agrobacterium rhizogenes causes transformed hairy roots to develop from the site of infection. Many transformed roots exhibit higher contents of secondary metabolites compared to wildtype roots. The purpose of this study was to obtain transformed root cultures containing rol-genes from in vivo grown Rhodiola sp. for future sustainable production of bioactive compounds. For the experiment, in vivo grown stems and leaves of R. rosea and two accessions of R. pachyclados were inoculated with A. rhizogenes strain ATCC43057 in sterile conditions. Subsequent growth of putatively transformed roots in simulated bioreactors including various concentrations of the auxin, indole acetic acid (IAA) were used to improve the growth of the roots. Of R. pachyclados accession no. 1, 41%±10 of inoculated stem explants developed roots. This was not significantly different from the controls of which 53%±0 developed roots. From R. rosea 20%±20 of stem explants developed roots with no control stem explants developing any roots. Of the inoculated leaf explants of R. rosea 15%±5 developed roots which were significantly more than the control leaf explants where no roots developed. No leaf explants of R. pachyclados developed any roots during the experiment. The putatively transformed roots transferred to simulated bioreactors increased in weight with no significant differences between the auxin treatments. The preliminary results indicate that the inoculation of Rhodiola sp. might have resulted in transformed roots. The control roots were indistinguishable from the roots of the inoculated explants, thus selecting transformants will rely solely on PCR tests.

AB - Rhodiola rosea, commonly known as roseroot, has since ancient times been used against depression and for improving mental abilities because of its bioactive compounds. Due to excessive exploitation, the natural populations have been declining. Natural transformation with root-loci (rol)-genes from wildtype Agrobacterium rhizogenes causes transformed hairy roots to develop from the site of infection. Many transformed roots exhibit higher contents of secondary metabolites compared to wildtype roots. The purpose of this study was to obtain transformed root cultures containing rol-genes from in vivo grown Rhodiola sp. for future sustainable production of bioactive compounds. For the experiment, in vivo grown stems and leaves of R. rosea and two accessions of R. pachyclados were inoculated with A. rhizogenes strain ATCC43057 in sterile conditions. Subsequent growth of putatively transformed roots in simulated bioreactors including various concentrations of the auxin, indole acetic acid (IAA) were used to improve the growth of the roots. Of R. pachyclados accession no. 1, 41%±10 of inoculated stem explants developed roots. This was not significantly different from the controls of which 53%±0 developed roots. From R. rosea 20%±20 of stem explants developed roots with no control stem explants developing any roots. Of the inoculated leaf explants of R. rosea 15%±5 developed roots which were significantly more than the control leaf explants where no roots developed. No leaf explants of R. pachyclados developed any roots during the experiment. The putatively transformed roots transferred to simulated bioreactors increased in weight with no significant differences between the auxin treatments. The preliminary results indicate that the inoculation of Rhodiola sp. might have resulted in transformed roots. The control roots were indistinguishable from the roots of the inoculated explants, thus selecting transformants will rely solely on PCR tests.

KW - Natural transformation

KW - Rol-genes

KW - Rosavin

KW - Roseroot

KW - Salidroside

U2 - 10.17660/ActaHortic.2015.1098.15

DO - 10.17660/ActaHortic.2015.1098.15

M3 - Article in proceedings

AN - SCOPUS:84951173276

T3 - Acta Horticulturae

SP - 143

EP - 150

BT - Proceedings of the International Symposium on Medicinal Plants and Natural Products

A2 - Ghaemghami, J.

A2 - Cuerrier, A.

PB - International Society for Horticultural Science

T2 - International Symposium on Medicinal Plants and Natural Products

Y2 - 17 June 2013 through 19 June 2013

ER -