Hyposensitization therapy with whole pollen extract or purified allergens monitored by immunoblotting

E Jarolim; F Matthiesen; P S Skov; M Tejkl; O Oesterballe; L K Poulsen

Hyposensitization therapy with whole pollen extract or purified allergens monitored by immunoblotting

E Jarolim, F Matthiesen, P S Skov, M Tejkl, O Oesterballe, L K Poulsen

4 Citations (Scopus)

Abstract

Patients allergic to grass pollen were hyposensitized with two major allergenic components or whole extract of timothy grass pollen. Specific IgE, IgG1, and IgG4 formed during immunotherapy were analyzed by immunoblotting. Similar antibody-binding patterns were observed in both patient groups. Inhibition experiments using allergenic components isolated by preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that all antigenic components of timothy grass pollen detected in immunoblot dispose of private and cross-reactive determinants for binding of human IgE. The worse clinical outcome of immunotherapy after hyposensitization with two cross-reactive components did, therefore, not correlate with a specific antibody formation pattern.

Original language	English
Journal	International Archives of Allergy and Applied Immunology
Volume	92
Issue number	3
Pages (from-to)	254-64
Number of pages	11
ISSN	0020-5915
Publication status	Published - 1990

Keywords

Allergens
Antibody Affinity
Cross Reactions
Desensitization, Immunologic
Electrophoresis, Polyacrylamide Gel
Histamine Release
Humans
Immunoblotting
Immunoglobulin E
Immunoglobulin G
Poaceae
Pollen
Journal Article

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@article{65a84928c65345c0ad85ffa8521d3795,

title = "Hyposensitization therapy with whole pollen extract or purified allergens monitored by immunoblotting",

abstract = "Patients allergic to grass pollen were hyposensitized with two major allergenic components or whole extract of timothy grass pollen. Specific IgE, IgG1, and IgG4 formed during immunotherapy were analyzed by immunoblotting. Similar antibody-binding patterns were observed in both patient groups. Inhibition experiments using allergenic components isolated by preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that all antigenic components of timothy grass pollen detected in immunoblot dispose of private and cross-reactive determinants for binding of human IgE. The worse clinical outcome of immunotherapy after hyposensitization with two cross-reactive components did, therefore, not correlate with a specific antibody formation pattern.",

keywords = "Allergens, Antibody Affinity, Cross Reactions, Desensitization, Immunologic, Electrophoresis, Polyacrylamide Gel, Histamine Release, Humans, Immunoblotting, Immunoglobulin E, Immunoglobulin G, Poaceae, Pollen, Journal Article",

author = "E Jarolim and F Matthiesen and Skov, {P S} and M Tejkl and O Oesterballe and Poulsen, {L K}",

year = "1990",

language = "English",

volume = "92",

pages = "254--64",

journal = "International Archives of Allergy and Applied Immunology",

issn = "0020-5915",

publisher = "S. Karger AG",

number = "3",

}

TY - JOUR

T1 - Hyposensitization therapy with whole pollen extract or purified allergens monitored by immunoblotting

AU - Jarolim, E

AU - Matthiesen, F

AU - Skov, P S

AU - Tejkl, M

AU - Oesterballe, O

AU - Poulsen, L K

PY - 1990

Y1 - 1990

N2 - Patients allergic to grass pollen were hyposensitized with two major allergenic components or whole extract of timothy grass pollen. Specific IgE, IgG1, and IgG4 formed during immunotherapy were analyzed by immunoblotting. Similar antibody-binding patterns were observed in both patient groups. Inhibition experiments using allergenic components isolated by preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that all antigenic components of timothy grass pollen detected in immunoblot dispose of private and cross-reactive determinants for binding of human IgE. The worse clinical outcome of immunotherapy after hyposensitization with two cross-reactive components did, therefore, not correlate with a specific antibody formation pattern.

AB - Patients allergic to grass pollen were hyposensitized with two major allergenic components or whole extract of timothy grass pollen. Specific IgE, IgG1, and IgG4 formed during immunotherapy were analyzed by immunoblotting. Similar antibody-binding patterns were observed in both patient groups. Inhibition experiments using allergenic components isolated by preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that all antigenic components of timothy grass pollen detected in immunoblot dispose of private and cross-reactive determinants for binding of human IgE. The worse clinical outcome of immunotherapy after hyposensitization with two cross-reactive components did, therefore, not correlate with a specific antibody formation pattern.

KW - Allergens

KW - Antibody Affinity

KW - Cross Reactions

KW - Desensitization, Immunologic

KW - Electrophoresis, Polyacrylamide Gel

KW - Histamine Release

KW - Humans

KW - Immunoblotting

KW - Immunoglobulin E

KW - Immunoglobulin G

KW - Poaceae

KW - Pollen

KW - Journal Article

M3 - Journal article

C2 - 1703516

SN - 0020-5915

VL - 92

SP - 254

EP - 264

JO - International Archives of Allergy and Applied Immunology

JF - International Archives of Allergy and Applied Immunology

IS - 3

ER -

Hyposensitization therapy with whole pollen extract or purified allergens monitored by immunoblotting

Abstract

Keywords

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