Hydroxyproline interference during the gas chromatographic analysis of D/L aspartic acid in human dentine

E. R. Waite; M. J. Collins; A. C.T. Van Duin

doi:10.1007/s004140050214

Hydroxyproline interference during the gas chromatographic analysis of D/L aspartic acid in human dentine

E. R. Waite^*, M. J. Collins, A. C.T. Van Duin

^*Corresponding author for this work

14 Citations (Scopus)

Abstract

The proportion of D- to L-enantiomers of aspartic acid in metabolically isolated proteins has been used by forensic scientists to estimate age at death. We have demonstrated the interference of a derivative of hydroxyproline (N-TFA isopropyl Hyp ester) with the NTFA isopropyl L-Aspartic (Asp) acid ester during gas chromatography of amino acids. This has serious implications for the accurate quantification of the D- to L-Asp ratio extracted from collagenous proteins. Having demonstrated the potential for this co-elution in amino acid standards, acid-soluble dentine proteins and non-mineralised collagen, we argue that this problem can be overcome either by high resolution separation or by analysis of the (Hyp-poor) non- collagenous protein fraction.

Original language	English
Journal	International Journal of Legal Medicine
Volume	112
Issue number	2
Pages (from-to)	124-131
Number of pages	8
ISSN	0937-9827
DOIs	https://doi.org/10.1007/s004140050214
Publication status	Published - 1 Jan 1999

Keywords

Collagen
D/L Aspartic acid
Gas chromatography
Hydroxyproline
N-TFA isopropyl ester derivatives

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10.1007/s004140050214

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title = "Hydroxyproline interference during the gas chromatographic analysis of D/L aspartic acid in human dentine",

abstract = "The proportion of D- to L-enantiomers of aspartic acid in metabolically isolated proteins has been used by forensic scientists to estimate age at death. We have demonstrated the interference of a derivative of hydroxyproline (N-TFA isopropyl Hyp ester) with the NTFA isopropyl L-Aspartic (Asp) acid ester during gas chromatography of amino acids. This has serious implications for the accurate quantification of the D- to L-Asp ratio extracted from collagenous proteins. Having demonstrated the potential for this co-elution in amino acid standards, acid-soluble dentine proteins and non-mineralised collagen, we argue that this problem can be overcome either by high resolution separation or by analysis of the (Hyp-poor) non- collagenous protein fraction.",

keywords = "Collagen, D/L Aspartic acid, Gas chromatography, Hydroxyproline, N-TFA isopropyl ester derivatives",

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T1 - Hydroxyproline interference during the gas chromatographic analysis of D/L aspartic acid in human dentine

AU - Waite, E. R.

AU - Collins, M. J.

AU - Van Duin, A. C.T.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The proportion of D- to L-enantiomers of aspartic acid in metabolically isolated proteins has been used by forensic scientists to estimate age at death. We have demonstrated the interference of a derivative of hydroxyproline (N-TFA isopropyl Hyp ester) with the NTFA isopropyl L-Aspartic (Asp) acid ester during gas chromatography of amino acids. This has serious implications for the accurate quantification of the D- to L-Asp ratio extracted from collagenous proteins. Having demonstrated the potential for this co-elution in amino acid standards, acid-soluble dentine proteins and non-mineralised collagen, we argue that this problem can be overcome either by high resolution separation or by analysis of the (Hyp-poor) non- collagenous protein fraction.

AB - The proportion of D- to L-enantiomers of aspartic acid in metabolically isolated proteins has been used by forensic scientists to estimate age at death. We have demonstrated the interference of a derivative of hydroxyproline (N-TFA isopropyl Hyp ester) with the NTFA isopropyl L-Aspartic (Asp) acid ester during gas chromatography of amino acids. This has serious implications for the accurate quantification of the D- to L-Asp ratio extracted from collagenous proteins. Having demonstrated the potential for this co-elution in amino acid standards, acid-soluble dentine proteins and non-mineralised collagen, we argue that this problem can be overcome either by high resolution separation or by analysis of the (Hyp-poor) non- collagenous protein fraction.

KW - Collagen

KW - D/L Aspartic acid

KW - Gas chromatography

KW - Hydroxyproline

KW - N-TFA isopropyl ester derivatives

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DO - 10.1007/s004140050214

M3 - Journal article

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SP - 124

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JO - International Journal of Legal Medicine

JF - International Journal of Legal Medicine

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ER -

Hydroxyproline interference during the gas chromatographic analysis of D/L aspartic acid in human dentine

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