Hyaluronidase, phospholipase A2 and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa

Marianne Molander Schmidt; Line Hagner Nielsen; Søren Vinter Søgaard; Dan Stærk; Nina Rønsted; Drissa Diallo; Chifundera Kusamba Zacharie; Johannes van Staden; Anna Jäger

doi:10.1016/j.jep.2014.09.027

Hyaluronidase, phospholipase A₂ and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa

Marianne Molander Schmidt, Line Hagner Nielsen, Søren Vinter Søgaard, Dan Stærk, Nina Rønsted, Drissa Diallo, Chifundera Kusamba Zacharie, Johannes van Staden, Anna Jäger

30 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Snakebite envenomation, every year, causes estimated 510,000 mortalities and results in more than 515,000 amputations in sub-Saharan Africa alone. Antiserum is not easily accessible in these regions or doctors are simply not available, thus more than 80% of all patients seek traditional practitioners as first-choice. Therefore it is important to investigate whether the plants used in traditional medicine systems contain compounds against the necrosis-inducing enzymes of snake venom.

Materials and methods: Extracts from traditionally used plants from DR Congo, Mali and South Africa were tested in hyaluronidase, phospholipase A2 and protease enzyme bioassays using Bitis arietans and Naja nigricollis as enzyme source.

Results: A total of 226 extracts from 94 different plant species from the three countries, Mali, Democratic Republic of Congo and South Africa were tested in phospholipase A2, proteases and hyaluronidase enzyme assays. Forty plant species showed more than 90% inhibition in one or more assay. Fabaceae, Anacardiaceae and Malvaceae were the families with the highest number of active species, and the active compounds were distributed in different plant parts depending on plant species. Polyphenols were removed in the search for specific enzyme inhibitors against hyaluronidase, phospholipase A2 or proteases from extracts with IC50 values below 100 ig!ml. Water extracts of Pupalia lappacea, Combretum molle, Strychnos innocua and Grewia mollis and ethanol extract of Lannea acida and Bauhinia thonningii still showed IC50 values below 100 igml in either the hyaluronidase or protease bioassay after removal of polyphenols.

Conclusion: As four of the active plants are widely distributed in the areas where the snake species Bitis arietans and Naja nigricollis occur a potential inhibitor of the necrotic enzymes is accessible for many people in sub-Saharan Africa.

Original language	English
Journal	Journal of Ethnopharmacology
Volume	157
Pages (from-to)	171-180
Number of pages	10
ISSN	0378-8741
DOIs	https://doi.org/10.1016/j.jep.2014.09.027
Publication status	Published - 18 Nov 2014

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10.1016/j.jep.2014.09.027

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Schmidt, M. M., Nielsen, L. H., Søgaard, S. V., Stærk, D., Rønsted, N., Diallo, D., Kusamba Zacharie, C., van Staden, J., & Jäger, A. (2014). Hyaluronidase, phospholipase A₂ and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa. Journal of Ethnopharmacology, 157, 171-180. https://doi.org/10.1016/j.jep.2014.09.027

Hyaluronidase, phospholipase A₂ and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa. / Schmidt, Marianne Molander; Nielsen, Line Hagner; Søgaard, Søren Vinter et al.
In: Journal of Ethnopharmacology, Vol. 157, 18.11.2014, p. 171-180.

Research output: Contribution to journal › Journal article › Research › peer-review

Schmidt, MM, Nielsen, LH, Søgaard, SV, Stærk, D , Rønsted, N, Diallo, D, Kusamba Zacharie, C, van Staden, J & Jäger, A 2014, 'Hyaluronidase, phospholipase A₂ and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa', Journal of Ethnopharmacology, vol. 157, pp. 171-180. https://doi.org/10.1016/j.jep.2014.09.027

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title = "Hyaluronidase, phospholipase A2 and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa",

abstract = "Ethnopharmacological relevance: Snakebite envenomation, every year, causes estimated 510,000 mortalities and results in more than 515,000 amputations in sub-Saharan Africa alone. Antiserum is not easily accessible in these regions or doctors are simply not available, thus more than 80% of all patients seek traditional practitioners as first-choice. Therefore it is important to investigate whether the plants used in traditional medicine systems contain compounds against the necrosis-inducing enzymes of snake venom.Materials and methods: Extracts from traditionally used plants from DR Congo, Mali and South Africa were tested in hyaluronidase, phospholipase A2 and protease enzyme bioassays using Bitis arietans and Naja nigricollis as enzyme source.Results: A total of 226 extracts from 94 different plant species from the three countries, Mali, Democratic Republic of Congo and South Africa were tested in phospholipase A2, proteases and hyaluronidase enzyme assays. Forty plant species showed more than 90% inhibition in one or more assay. Fabaceae, Anacardiaceae and Malvaceae were the families with the highest number of active species, and the active compounds were distributed in different plant parts depending on plant species. Polyphenols were removed in the search for specific enzyme inhibitors against hyaluronidase, phospholipase A2 or proteases from extracts with IC50 values below 100 ig!ml. Water extracts of Pupalia lappacea, Combretum molle, Strychnos innocua and Grewia mollis and ethanol extract of Lannea acida and Bauhinia thonningii still showed IC50 values below 100 igml in either the hyaluronidase or protease bioassay after removal of polyphenols.Conclusion: As four of the active plants are widely distributed in the areas where the snake species Bitis arietans and Naja nigricollis occur a potential inhibitor of the necrotic enzymes is accessible for many people in sub-Saharan Africa.",

author = "Schmidt, {Marianne Molander} and Nielsen, {Line Hagner} and S{\o}gaard, {S{\o}ren Vinter} and Dan St{\ae}rk and Nina R{\o}nsted and Drissa Diallo and {Kusamba Zacharie}, Chifundera and {van Staden}, Johannes and Anna J{\"a}ger",

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month = nov,

day = "18",

doi = "10.1016/j.jep.2014.09.027",

language = "English",

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T1 - Hyaluronidase, phospholipase A2 and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa

AU - Schmidt, Marianne Molander

AU - Nielsen, Line Hagner

AU - Søgaard, Søren Vinter

AU - Stærk, Dan

AU - Rønsted, Nina

AU - Diallo, Drissa

AU - Kusamba Zacharie, Chifundera

AU - van Staden, Johannes

AU - Jäger, Anna

PY - 2014/11/18

Y1 - 2014/11/18

N2 - Ethnopharmacological relevance: Snakebite envenomation, every year, causes estimated 510,000 mortalities and results in more than 515,000 amputations in sub-Saharan Africa alone. Antiserum is not easily accessible in these regions or doctors are simply not available, thus more than 80% of all patients seek traditional practitioners as first-choice. Therefore it is important to investigate whether the plants used in traditional medicine systems contain compounds against the necrosis-inducing enzymes of snake venom.Materials and methods: Extracts from traditionally used plants from DR Congo, Mali and South Africa were tested in hyaluronidase, phospholipase A2 and protease enzyme bioassays using Bitis arietans and Naja nigricollis as enzyme source.Results: A total of 226 extracts from 94 different plant species from the three countries, Mali, Democratic Republic of Congo and South Africa were tested in phospholipase A2, proteases and hyaluronidase enzyme assays. Forty plant species showed more than 90% inhibition in one or more assay. Fabaceae, Anacardiaceae and Malvaceae were the families with the highest number of active species, and the active compounds were distributed in different plant parts depending on plant species. Polyphenols were removed in the search for specific enzyme inhibitors against hyaluronidase, phospholipase A2 or proteases from extracts with IC50 values below 100 ig!ml. Water extracts of Pupalia lappacea, Combretum molle, Strychnos innocua and Grewia mollis and ethanol extract of Lannea acida and Bauhinia thonningii still showed IC50 values below 100 igml in either the hyaluronidase or protease bioassay after removal of polyphenols.Conclusion: As four of the active plants are widely distributed in the areas where the snake species Bitis arietans and Naja nigricollis occur a potential inhibitor of the necrotic enzymes is accessible for many people in sub-Saharan Africa.

AB - Ethnopharmacological relevance: Snakebite envenomation, every year, causes estimated 510,000 mortalities and results in more than 515,000 amputations in sub-Saharan Africa alone. Antiserum is not easily accessible in these regions or doctors are simply not available, thus more than 80% of all patients seek traditional practitioners as first-choice. Therefore it is important to investigate whether the plants used in traditional medicine systems contain compounds against the necrosis-inducing enzymes of snake venom.Materials and methods: Extracts from traditionally used plants from DR Congo, Mali and South Africa were tested in hyaluronidase, phospholipase A2 and protease enzyme bioassays using Bitis arietans and Naja nigricollis as enzyme source.Results: A total of 226 extracts from 94 different plant species from the three countries, Mali, Democratic Republic of Congo and South Africa were tested in phospholipase A2, proteases and hyaluronidase enzyme assays. Forty plant species showed more than 90% inhibition in one or more assay. Fabaceae, Anacardiaceae and Malvaceae were the families with the highest number of active species, and the active compounds were distributed in different plant parts depending on plant species. Polyphenols were removed in the search for specific enzyme inhibitors against hyaluronidase, phospholipase A2 or proteases from extracts with IC50 values below 100 ig!ml. Water extracts of Pupalia lappacea, Combretum molle, Strychnos innocua and Grewia mollis and ethanol extract of Lannea acida and Bauhinia thonningii still showed IC50 values below 100 igml in either the hyaluronidase or protease bioassay after removal of polyphenols.Conclusion: As four of the active plants are widely distributed in the areas where the snake species Bitis arietans and Naja nigricollis occur a potential inhibitor of the necrotic enzymes is accessible for many people in sub-Saharan Africa.

U2 - 10.1016/j.jep.2014.09.027

DO - 10.1016/j.jep.2014.09.027

M3 - Journal article

C2 - 25256691

SN - 0378-8741

VL - 157

SP - 171

EP - 180

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

ER -

Hyaluronidase, phospholipase A2 and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa

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Hyaluronidase, phospholipase A₂ and protease inhibitory activity of plants used in traditional treatment of snakebite-induced tissue necrosis in Mali, DR Congo and South Africa