TY - JOUR
T1 - Human skeletal muscle perilipin 2 and 3 expression varies with insulin sensitivity
AU - Vigelsø Hansen, Andreas
AU - Prats Gavalda, Clara
AU - Ploug, Thorkil
AU - Dela, Flemming
AU - Helge, Jørn Wulff
PY - 2013/5
Y1 - 2013/5
N2 - Background: Impaired insulin sensitivity may partly arise from a dysregulated lipid metabolism in human skeletal muscle. This study investigates the expression levels of perilipin 2, 3, and 5, and four key lipases in human skeletal muscle from the subjects that exhibit a range from normal to very low insulin sensitivity. Methods: 25 middle aged male participants were matched for lean body mass and recruited into three groups; type 2 diabetes patients (T2D), impaired glu- cose tolerance (IGT), and healthy sedentary controls (CON) according to their glucose tolerance and VO2peak. A muscle biopsy was obtained from vastus lateralis, and a two-step sequential euglycaemic-hy- perinsulinaemic clamp was performed. Muscle sam- ples were analyzed by Western blot for expression of perilipin 2, 3, 5, adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), endothelial lipase (EL) and lipoprotein lipase (LPL). Results: Perilipin 3 expression was higher in T2D compared to CON. Perilipin 2 expression was higher in CON than T2D. We observed no difference in expression of perilipin 5, ATGL, HSL, EL or LPL between the groups. Con- clusions: In the present study the muscle perilipin 3 expression and perilipin 2 expression varied mark- edly with insulin sensitivity. This difference in perili- pin expression may indicate that the lipid droplet function and thus storage and release of fatty acid- vary with insulin sensitivity.
AB - Background: Impaired insulin sensitivity may partly arise from a dysregulated lipid metabolism in human skeletal muscle. This study investigates the expression levels of perilipin 2, 3, and 5, and four key lipases in human skeletal muscle from the subjects that exhibit a range from normal to very low insulin sensitivity. Methods: 25 middle aged male participants were matched for lean body mass and recruited into three groups; type 2 diabetes patients (T2D), impaired glu- cose tolerance (IGT), and healthy sedentary controls (CON) according to their glucose tolerance and VO2peak. A muscle biopsy was obtained from vastus lateralis, and a two-step sequential euglycaemic-hy- perinsulinaemic clamp was performed. Muscle sam- ples were analyzed by Western blot for expression of perilipin 2, 3, 5, adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), endothelial lipase (EL) and lipoprotein lipase (LPL). Results: Perilipin 3 expression was higher in T2D compared to CON. Perilipin 2 expression was higher in CON than T2D. We observed no difference in expression of perilipin 5, ATGL, HSL, EL or LPL between the groups. Con- clusions: In the present study the muscle perilipin 3 expression and perilipin 2 expression varied mark- edly with insulin sensitivity. This difference in perili- pin expression may indicate that the lipid droplet function and thus storage and release of fatty acid- vary with insulin sensitivity.
KW - Faculty of Health and Medical Sciences
KW - Lipid Metabolism
KW - Insulin Sensitivity
KW - Perilipin Proteins
KW - Lipolysis
KW - Human Skeletal Muscle
KW - Lipotoxicity
U2 - 10.4236/jbise.2013.65A010
DO - 10.4236/jbise.2013.65A010
M3 - Journal article
SN - 1937-6871
VL - 6
SP - 65
EP - 72
JO - Journal of Biomedical Science and Engineering
JF - Journal of Biomedical Science and Engineering
IS - 5A
ER -
