Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

M Skovbro; M Baranowski; C Skov-Jensen; A Flint; F Dela; J Gorski; J W Helge

doi:10.1007/s00125-008-1014-z

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

M Skovbro, M Baranowski, C Skov-Jensen, A Flint, F Dela, J Gorski, J W Helge

104 Citations (Scopus)

Abstract

AIMS/HYPOTHESIS: In skeletal muscle, ceramides may be involved in the pathogenesis of insulin resistance through an attenuation of insulin signalling. This study investigated total skeletal muscle ceramide fatty acid content in participants exhibiting a wide range of insulin sensitivities. METHODS: The middle-aged male participants (n=33) were matched for lean body mass and divided into four groups: type 2 diabetes (T2D, n=8), impaired glucose tolerance (IGT, n=9), healthy controls (CON, n=8) and endurance-trained (TR, n=8). A two step (28 and 80 mU m(-2) min(-1)) sequential euglycaemic-hyperinsulinaemic clamp was performed for 120 and 90 min for step 1 and step 2, respectively. Muscle biopsies were obtained from vastus lateralis at baseline, and after steps 1 and 2. RESULTS: Glucose infusion rates increased in response to insulin infusion, and significant differences were present between groups (T2D

Original language	English
Journal	Diabetologia
Volume	51
Issue number	7
Pages (from-to)	1253-60
Number of pages	7
ISSN	0012-186X
DOIs	https://doi.org/10.1007/s00125-008-1014-z
Publication status	Published - 2008

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10.1007/s00125-008-1014-z

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@article{8851dd00f1f911ddbf70000ea68e967b,

title = "Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity",

abstract = "AIMS/HYPOTHESIS: In skeletal muscle, ceramides may be involved in the pathogenesis of insulin resistance through an attenuation of insulin signalling. This study investigated total skeletal muscle ceramide fatty acid content in participants exhibiting a wide range of insulin sensitivities. METHODS: The middle-aged male participants (n=33) were matched for lean body mass and divided into four groups: type 2 diabetes (T2D, n=8), impaired glucose tolerance (IGT, n=9), healthy controls (CON, n=8) and endurance-trained (TR, n=8). A two step (28 and 80 mU m(-2) min(-1)) sequential euglycaemic-hyperinsulinaemic clamp was performed for 120 and 90 min for step 1 and step 2, respectively. Muscle biopsies were obtained from vastus lateralis at baseline, and after steps 1 and 2. RESULTS: Glucose infusion rates increased in response to insulin infusion, and significant differences were present between groups (T2D",

author = "M Skovbro and M Baranowski and C Skov-Jensen and A Flint and F Dela and J Gorski and Helge, {J W}",

note = "Keywords: Biopsy; Blood Glucose; Ceramides; Diabetes Mellitus, Type 2; Diglycerides; Glucose Clamp Technique; Glucose Intolerance; Humans; Hyperinsulinism; Insulin Resistance; Male; Middle Aged; Muscle, Skeletal; Oxygen Consumption; Physical Endurance; Triglycerides",

year = "2008",

doi = "10.1007/s00125-008-1014-z",

language = "English",

volume = "51",

pages = "1253--60",

journal = "Diabetologia",

issn = "0012-186X",

publisher = "Springer",

number = "7",

}

TY - JOUR

T1 - Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

AU - Skovbro, M

AU - Baranowski, M

AU - Skov-Jensen, C

AU - Flint, A

AU - Dela, F

AU - Gorski, J

AU - Helge, J W

N1 - Keywords: Biopsy; Blood Glucose; Ceramides; Diabetes Mellitus, Type 2; Diglycerides; Glucose Clamp Technique; Glucose Intolerance; Humans; Hyperinsulinism; Insulin Resistance; Male; Middle Aged; Muscle, Skeletal; Oxygen Consumption; Physical Endurance; Triglycerides

PY - 2008

Y1 - 2008

N2 - AIMS/HYPOTHESIS: In skeletal muscle, ceramides may be involved in the pathogenesis of insulin resistance through an attenuation of insulin signalling. This study investigated total skeletal muscle ceramide fatty acid content in participants exhibiting a wide range of insulin sensitivities. METHODS: The middle-aged male participants (n=33) were matched for lean body mass and divided into four groups: type 2 diabetes (T2D, n=8), impaired glucose tolerance (IGT, n=9), healthy controls (CON, n=8) and endurance-trained (TR, n=8). A two step (28 and 80 mU m(-2) min(-1)) sequential euglycaemic-hyperinsulinaemic clamp was performed for 120 and 90 min for step 1 and step 2, respectively. Muscle biopsies were obtained from vastus lateralis at baseline, and after steps 1 and 2. RESULTS: Glucose infusion rates increased in response to insulin infusion, and significant differences were present between groups (T2D

AB - AIMS/HYPOTHESIS: In skeletal muscle, ceramides may be involved in the pathogenesis of insulin resistance through an attenuation of insulin signalling. This study investigated total skeletal muscle ceramide fatty acid content in participants exhibiting a wide range of insulin sensitivities. METHODS: The middle-aged male participants (n=33) were matched for lean body mass and divided into four groups: type 2 diabetes (T2D, n=8), impaired glucose tolerance (IGT, n=9), healthy controls (CON, n=8) and endurance-trained (TR, n=8). A two step (28 and 80 mU m(-2) min(-1)) sequential euglycaemic-hyperinsulinaemic clamp was performed for 120 and 90 min for step 1 and step 2, respectively. Muscle biopsies were obtained from vastus lateralis at baseline, and after steps 1 and 2. RESULTS: Glucose infusion rates increased in response to insulin infusion, and significant differences were present between groups (T2D

U2 - 10.1007/s00125-008-1014-z

DO - 10.1007/s00125-008-1014-z

M3 - Journal article

C2 - 18458871

SN - 0012-186X

VL - 51

SP - 1253

EP - 1260

JO - Diabetologia

JF - Diabetologia

IS - 7

ER -

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

Abstract

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