Human and rodent muscle Na(+)-K(+)-ATPase in diabetes related to insulin, starvation, and training

TY - JOUR

T1 - Human and rodent muscle Na(+)-K(+)-ATPase in diabetes related to insulin, starvation, and training

AU - Schmidt, T A

AU - Hasselbalch, S

AU - Farrell, P A

AU - Vestergaard, H

AU - Kjeldsen, K

PY - 1994

Y1 - 1994

N2 - As determined by vanadate-facilitated [3H]ouabain binding to intact samples, semistarvation and untreated streptozotocin- or partial pancreatectomy-induced diabetes reduced rat soleus muscle Na(+)-K(+)-adenosinetriphosphatase (Na(+)-K(+)-ATPase) concentration by 12-21% (P < 0.05). Conversely, insulin treatment of rats with streptozotocin-induced diabetes induced an increase of 18-26% above control (P < 0.05). Treadmill training diminished the reduction in muscle [3H]ouabain binding site concentration induced by untreated diabetes to only 2-5%. No significant variation was observed in rat cerebral cortex Na(+)-K(+)-ATPase concentration as a result of diabetes, semistarvation, or insulin treatment. In human subjects, Na(+)-K(+)-ATPase concentration in vastus lateralis muscle biopsies was 17 and 22% greater (P < 0.05), respectively, in patients with treated non-insulin-dependent diabetes mellitus (n = 24) and insulin-dependent diabetes mellitus (n = 7) than in control subjects (n = 8). A positive linear correlation between muscle Na(+)-K(+)-ATPase and plasma insulin concentrations was observed (r = 0.50, P = 0.006; n = 29). Thus, insulin seems a regulator of muscle Na(+)-K(+)-ATPase concentration, reduction of muscle Na(+)-K(+)-ATPase concentration with untreated diabetes bears similarities with undernourishment, and physical conditioning may ameliorate the muscle Na(+)-K(+)-ATPase concentration decrease induced by diabetes.

AB - As determined by vanadate-facilitated [3H]ouabain binding to intact samples, semistarvation and untreated streptozotocin- or partial pancreatectomy-induced diabetes reduced rat soleus muscle Na(+)-K(+)-adenosinetriphosphatase (Na(+)-K(+)-ATPase) concentration by 12-21% (P < 0.05). Conversely, insulin treatment of rats with streptozotocin-induced diabetes induced an increase of 18-26% above control (P < 0.05). Treadmill training diminished the reduction in muscle [3H]ouabain binding site concentration induced by untreated diabetes to only 2-5%. No significant variation was observed in rat cerebral cortex Na(+)-K(+)-ATPase concentration as a result of diabetes, semistarvation, or insulin treatment. In human subjects, Na(+)-K(+)-ATPase concentration in vastus lateralis muscle biopsies was 17 and 22% greater (P < 0.05), respectively, in patients with treated non-insulin-dependent diabetes mellitus (n = 24) and insulin-dependent diabetes mellitus (n = 7) than in control subjects (n = 8). A positive linear correlation between muscle Na(+)-K(+)-ATPase and plasma insulin concentrations was observed (r = 0.50, P = 0.006; n = 29). Thus, insulin seems a regulator of muscle Na(+)-K(+)-ATPase concentration, reduction of muscle Na(+)-K(+)-ATPase concentration with untreated diabetes bears similarities with undernourishment, and physical conditioning may ameliorate the muscle Na(+)-K(+)-ATPase concentration decrease induced by diabetes.

KW - Adult

KW - Animals

KW - Cerebral Cortex

KW - Diabetes Mellitus

KW - Diabetes Mellitus, Experimental

KW - Diabetes Mellitus, Type 1

KW - Diabetes Mellitus, Type 2

KW - Female

KW - Humans

KW - Insulin

KW - Male

KW - Middle Aged

KW - Muscles

KW - Ouabain

KW - Pancreatectomy

KW - Physical Conditioning, Animal

KW - Rats

KW - Rats, Sprague-Dawley

KW - Rats, Wistar

KW - Sodium-Potassium-Exchanging ATPase

KW - Starvation

KW - Streptozocin

M3 - Journal article

C2 - 8063678

SN - 8750-7587

VL - 76

SP - 2140

EP - 2146

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 5

ER -