Histamine immunocytochemistry: a new method for detection of basophils in peripheral blood

H H Jacobi; O Johansson; Y Liang; H V Nielsen; C Thygesen; J B Hansen; T Jinquan; P S Skov; Lars K. Poulsen

Histamine immunocytochemistry: a new method for detection of basophils in peripheral blood

H H Jacobi, O Johansson, Y Liang, H V Nielsen, C Thygesen, J B Hansen, T Jinquan, P S Skov, Lars K. Poulsen

4 Citations (Scopus)

Abstract

We report that basophils in peripheral blood can be stained using histamine immunocytochemistry. The staining is based on the fixation of leucocytes with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (CDI) and the subsequent incubation of these cells with antisera raised against histamine conjugated to different carrier proteins using CDI. The staining appears to be specific for basophils and stained cells can be examined using both fluorescence microscopy and flow cytometry. In addition, histamine immunocytochemistry can be combined with conventional immunocytochemistry by incubating leucocytes with antibodies to cell surface antigens prior to or following fixation of the cells with CDI. Thus, histamine immunocytochemistry may be a valuable tool in future studies of human basophils.

Original language	English
Journal	Journal of Immunological Methods
Volume	237
Issue number	1-2
Pages (from-to)	29-37
Number of pages	9
ISSN	0022-1759
Publication status	Published - 3 Apr 2000

Keywords

Alcian Blue
Antibodies, Monoclonal
Antigens, CD
Basophils
Coloring Agents
Ethyldimethylaminopropyl Carbodiimide
Evaluation Studies as Topic
Flow Cytometry
Histamine
Humans
Immunohistochemistry
Leukocytes
Microscopy, Fluorescence
Staining and Labeling

Cite this

@article{df70158fb202482fb6d8f0af86991202,

title = "Histamine immunocytochemistry: a new method for detection of basophils in peripheral blood",

abstract = "We report that basophils in peripheral blood can be stained using histamine immunocytochemistry. The staining is based on the fixation of leucocytes with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (CDI) and the subsequent incubation of these cells with antisera raised against histamine conjugated to different carrier proteins using CDI. The staining appears to be specific for basophils and stained cells can be examined using both fluorescence microscopy and flow cytometry. In addition, histamine immunocytochemistry can be combined with conventional immunocytochemistry by incubating leucocytes with antibodies to cell surface antigens prior to or following fixation of the cells with CDI. Thus, histamine immunocytochemistry may be a valuable tool in future studies of human basophils.",

keywords = "Alcian Blue, Antibodies, Monoclonal, Antigens, CD, Basophils, Coloring Agents, Ethyldimethylaminopropyl Carbodiimide, Evaluation Studies as Topic, Flow Cytometry, Histamine, Humans, Immunohistochemistry, Leukocytes, Microscopy, Fluorescence, Staining and Labeling",

author = "Jacobi, {H H} and O Johansson and Y Liang and Nielsen, {H V} and C Thygesen and Hansen, {J B} and T Jinquan and Skov, {P S} and Poulsen, {Lars K.}",

year = "2000",

month = apr,

day = "3",

language = "English",

volume = "237",

pages = "29--37",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Histamine immunocytochemistry

T2 - a new method for detection of basophils in peripheral blood

AU - Jacobi, H H

AU - Johansson, O

AU - Liang, Y

AU - Nielsen, H V

AU - Thygesen, C

AU - Hansen, J B

AU - Jinquan, T

AU - Skov, P S

AU - Poulsen, Lars K.

PY - 2000/4/3

Y1 - 2000/4/3

N2 - We report that basophils in peripheral blood can be stained using histamine immunocytochemistry. The staining is based on the fixation of leucocytes with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (CDI) and the subsequent incubation of these cells with antisera raised against histamine conjugated to different carrier proteins using CDI. The staining appears to be specific for basophils and stained cells can be examined using both fluorescence microscopy and flow cytometry. In addition, histamine immunocytochemistry can be combined with conventional immunocytochemistry by incubating leucocytes with antibodies to cell surface antigens prior to or following fixation of the cells with CDI. Thus, histamine immunocytochemistry may be a valuable tool in future studies of human basophils.

AB - We report that basophils in peripheral blood can be stained using histamine immunocytochemistry. The staining is based on the fixation of leucocytes with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (CDI) and the subsequent incubation of these cells with antisera raised against histamine conjugated to different carrier proteins using CDI. The staining appears to be specific for basophils and stained cells can be examined using both fluorescence microscopy and flow cytometry. In addition, histamine immunocytochemistry can be combined with conventional immunocytochemistry by incubating leucocytes with antibodies to cell surface antigens prior to or following fixation of the cells with CDI. Thus, histamine immunocytochemistry may be a valuable tool in future studies of human basophils.

KW - Alcian Blue

KW - Antibodies, Monoclonal

KW - Antigens, CD

KW - Basophils

KW - Coloring Agents

KW - Ethyldimethylaminopropyl Carbodiimide

KW - Evaluation Studies as Topic

KW - Flow Cytometry

KW - Histamine

KW - Humans

KW - Immunohistochemistry

KW - Leukocytes

KW - Microscopy, Fluorescence

KW - Staining and Labeling

M3 - Journal article

C2 - 10725449

SN - 0022-1759

VL - 237

SP - 29

EP - 37

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Histamine immunocytochemistry: a new method for detection of basophils in peripheral blood

Abstract

Keywords

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