HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana

TY - JOUR

T1 - HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana

AU - Cho, Seok Keun

AU - Ryu, Moon Young

AU - Poulsen, Christian

AU - Kim, Jong Hum

AU - Oh, Tae Rin

AU - Choi, Suk Won

AU - Kim, Mijung

AU - Yang, Jun-Yi

AU - Boo, Kyung Hwan

AU - Geshi, Naomi

AU - Kim, Woo Taek

AU - Yang, Seong Wook

N1 - © 2017 Federation of European Biochemical Societies.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.

AB - A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.

KW - Letter

U2 - 10.1002/1873-3468.12628

DO - 10.1002/1873-3468.12628

M3 - Journal article

C2 - 28321834

SN - 0014-5793

VL - 591

SP - 1383

EP - 1393

JO - F E B S Letters

JF - F E B S Letters

IS - 10

ER -