High-throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity-reducing process

TY - JOUR

T1 - High-throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity-reducing process

AU - Huang, Yuhong

AU - Willats, William George Tycho

AU - Lange, Lene

AU - Jin, Yanling

AU - Fang, Yang

AU - Asunción Salmeán, Armando

AU - Pedersen, Henriette Lodberg

AU - Busk, Peter Kamp

AU - Zhao, Hai

N1 - © 2015 International Union of Biochemistry and Molecular Biology, Inc.

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Viscosity reduction has a great impact on the efficiency of ethanol production when using roots and tubers as feedstock. Plant cell wall-degrading enzymes have been successfully applied to overcome the challenges posed by high viscosity. However, the changes in cell wall polymers during the viscosity-reducing process are poorly characterized. Comprehensive microarray polymer profiling, which is a high-throughput microarray, was used for the first time to map changes in the cell wall polymers of sweet potato (Ipomoea batatas), cassava (Manihot esculenta), and Canna edulis Ker. over the entire viscosity-reducing process. The results indicated that the composition of cell wall polymers among these three roots and tubers was markedly different. The gel-like matrix and glycoprotein network in the C. edulis Ker. cell wall caused difficulty in viscosity reduction. The obvious viscosity reduction of the sweet potato and the cassava was attributed to the degradation of homogalacturonan and the released 1,4-β-d-galactan and 1,5-α-l-arabinan.

AB - Viscosity reduction has a great impact on the efficiency of ethanol production when using roots and tubers as feedstock. Plant cell wall-degrading enzymes have been successfully applied to overcome the challenges posed by high viscosity. However, the changes in cell wall polymers during the viscosity-reducing process are poorly characterized. Comprehensive microarray polymer profiling, which is a high-throughput microarray, was used for the first time to map changes in the cell wall polymers of sweet potato (Ipomoea batatas), cassava (Manihot esculenta), and Canna edulis Ker. over the entire viscosity-reducing process. The results indicated that the composition of cell wall polymers among these three roots and tubers was markedly different. The gel-like matrix and glycoprotein network in the C. edulis Ker. cell wall caused difficulty in viscosity reduction. The obvious viscosity reduction of the sweet potato and the cassava was attributed to the degradation of homogalacturonan and the released 1,4-β-d-galactan and 1,5-α-l-arabinan.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/bab.1367

DO - 10.1002/bab.1367

M3 - Journal article

C2 - 25757626

SN - 0885-4513

VL - 63

SP - 178

EP - 189

JO - Biotechnology and Applied Biochemistry

JF - Biotechnology and Applied Biochemistry

IS - 2

ER -