High plasma thiocyanate levels modulate protein damage induced by myeloperoxidase and perturb measurement of 3-chlorotyrosine

TY - JOUR

T1 - High plasma thiocyanate levels modulate protein damage induced by myeloperoxidase and perturb measurement of 3-chlorotyrosine

AU - Talib, Jihan

AU - Pattison, David I

AU - Harmer, Jason A

AU - Celermajer, David S

AU - Davies, Michael Jonathan

N1 - Copyright © 2012 Elsevier Inc. All rights reserved.

PY - 2012/7/1

Y1 - 2012/7/1

N2 - Smokers have an elevated risk of atherosclerosis but the origin of this elevated risk is incompletely defined, though increasing evidence supports a role for the oxidant-generating enzyme myeloperoxidase (MPO). In previous studies we have demonstrated that smokers have elevated levels of thiocyanate ions (SCN -), relative to nonsmokers, and increased thiol oxidation, as SCN - is a favored substrate for MPO, and the resulting hypothiocyanous acid (HOSCN) targets thiol groups rapidly and selectively. In this study we show that increased HOSCN formation by MPO diminishes damage to nonthiol targets on both model proteins and human plasma proteins. Thus high SCN - levels protect against HOCl- and MPO-mediated damage to methionine, tryptophan, lysine, histidine, and tyrosine residues on proteins. Furthermore, levels of the HOCl-mediated marker compound 3-chlorotyrosine and the cross-linked product dityrosine are decreased. Plasma protein 3-chlorotyrosine levels induced by HOCl exposure in nonsmokers are elevated over the levels detected in smokers when exposed to identical oxidative insult (P<0.05), and a strong inverse correlation exists between plasma SCN - levels and 3-chlorotyrosine concentrations (r=0.6182; P<0.0001). These correlations were also significant for smokers (r=0.2724; P<0.05) and nonsmokers (r=0.4141; P<0.01) when analyzed as individual groups. These data indicate that plasma SCN - levels are a key determinant of the extent and type of protein oxidation induced by MPO on isolated and plasma proteins and that smoking status and resulting high SCN - levels can markedly modulate the levels of the widely used biomarker compound 3-chlorotyrosine.

AB - Smokers have an elevated risk of atherosclerosis but the origin of this elevated risk is incompletely defined, though increasing evidence supports a role for the oxidant-generating enzyme myeloperoxidase (MPO). In previous studies we have demonstrated that smokers have elevated levels of thiocyanate ions (SCN -), relative to nonsmokers, and increased thiol oxidation, as SCN - is a favored substrate for MPO, and the resulting hypothiocyanous acid (HOSCN) targets thiol groups rapidly and selectively. In this study we show that increased HOSCN formation by MPO diminishes damage to nonthiol targets on both model proteins and human plasma proteins. Thus high SCN - levels protect against HOCl- and MPO-mediated damage to methionine, tryptophan, lysine, histidine, and tyrosine residues on proteins. Furthermore, levels of the HOCl-mediated marker compound 3-chlorotyrosine and the cross-linked product dityrosine are decreased. Plasma protein 3-chlorotyrosine levels induced by HOCl exposure in nonsmokers are elevated over the levels detected in smokers when exposed to identical oxidative insult (P<0.05), and a strong inverse correlation exists between plasma SCN - levels and 3-chlorotyrosine concentrations (r=0.6182; P<0.0001). These correlations were also significant for smokers (r=0.2724; P<0.05) and nonsmokers (r=0.4141; P<0.01) when analyzed as individual groups. These data indicate that plasma SCN - levels are a key determinant of the extent and type of protein oxidation induced by MPO on isolated and plasma proteins and that smoking status and resulting high SCN - levels can markedly modulate the levels of the widely used biomarker compound 3-chlorotyrosine.

KW - Biological Markers

KW - Blood Proteins

KW - Case-Control Studies

KW - Female

KW - Humans

KW - Male

KW - Oxidants

KW - Oxidation-Reduction

KW - Peroxidase

KW - Smoking

KW - Sulfhydryl Compounds

KW - Thiocyanates

KW - Tyrosine

U2 - 10.1016/j.freeradbiomed.2012.04.018

DO - 10.1016/j.freeradbiomed.2012.04.018

M3 - Journal article

C2 - 22609005

SN - 0891-5849

VL - 53

SP - 20

EP - 29

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

IS - 1

ER -