High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L

S. H. Hansen; A. G. Jensen; Claus Cornett; I. Bjornsdottir; S. Taylor; B. Wright; I. D. Wilson

High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L

S. H. Hansen, A. G. Jensen, Claus Cornett, I. Bjornsdottir, S. Taylor, B. Wright, I. D. Wilson

125 Citations (Scopus)

Abstract

The on-line separation and structure elucidation of naphthodianthrones, flavonoids, and other constituents of an extract from Hypericum perforatum L, using high performance liquid chromatography (HPLC) coupled on-line with ultraviolet-visible, nuclear magnetic resonance (NMR), and mass spectrometry (MS) is described. A conventional reversed-phase HPLC system using ammonium acetate as the buffer substance in the eluent tvas used, and proton NMR spectra were obtained on a 500 MHz NMR instrument. The MS and MS/MS analyses were performed using negative electrospray ionization, In the present study, all of the major known constituents in extracts from Hypericum perforatum L, were identified, and two new substances which had not previously been reported as constituents of extracts of Hypericum perforatum L. were identified and their structures elucidated.

Original language	Undefined/Unknown
Journal	Analytical Chemistry
Volume	71
Issue number	22
Pages (from-to)	5235-5241
Number of pages	7
ISSN	0003-2700
Publication status	Published - 1999

Cite this

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title = "High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L",

abstract = "The on-line separation and structure elucidation of naphthodianthrones, flavonoids, and other constituents of an extract from Hypericum perforatum L, using high performance liquid chromatography (HPLC) coupled on-line with ultraviolet-visible, nuclear magnetic resonance (NMR), and mass spectrometry (MS) is described. A conventional reversed-phase HPLC system using ammonium acetate as the buffer substance in the eluent tvas used, and proton NMR spectra were obtained on a 500 MHz NMR instrument. The MS and MS/MS analyses were performed using negative electrospray ionization, In the present study, all of the major known constituents in extracts from Hypericum perforatum L, were identified, and two new substances which had not previously been reported as constituents of extracts of Hypericum perforatum L. were identified and their structures elucidated.",

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T1 - High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L

AU - Hansen, S. H.

AU - Jensen, A. G.

AU - Cornett, Claus

AU - Bjornsdottir, I.

AU - Taylor, S.

AU - Wright, B.

AU - Wilson, I. D.

PY - 1999

Y1 - 1999

N2 - The on-line separation and structure elucidation of naphthodianthrones, flavonoids, and other constituents of an extract from Hypericum perforatum L, using high performance liquid chromatography (HPLC) coupled on-line with ultraviolet-visible, nuclear magnetic resonance (NMR), and mass spectrometry (MS) is described. A conventional reversed-phase HPLC system using ammonium acetate as the buffer substance in the eluent tvas used, and proton NMR spectra were obtained on a 500 MHz NMR instrument. The MS and MS/MS analyses were performed using negative electrospray ionization, In the present study, all of the major known constituents in extracts from Hypericum perforatum L, were identified, and two new substances which had not previously been reported as constituents of extracts of Hypericum perforatum L. were identified and their structures elucidated.

AB - The on-line separation and structure elucidation of naphthodianthrones, flavonoids, and other constituents of an extract from Hypericum perforatum L, using high performance liquid chromatography (HPLC) coupled on-line with ultraviolet-visible, nuclear magnetic resonance (NMR), and mass spectrometry (MS) is described. A conventional reversed-phase HPLC system using ammonium acetate as the buffer substance in the eluent tvas used, and proton NMR spectra were obtained on a 500 MHz NMR instrument. The MS and MS/MS analyses were performed using negative electrospray ionization, In the present study, all of the major known constituents in extracts from Hypericum perforatum L, were identified, and two new substances which had not previously been reported as constituents of extracts of Hypericum perforatum L. were identified and their structures elucidated.

KW - identification products

M3 - Tidsskriftartikel

SN - 0003-2700

VL - 71

SP - 5235

EP - 5241

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 22

ER -