High expression of the taurine transporter TauT in primary cilia of NIH3T3 fibroblasts

Søren Tvorup Christensen; Jesper W. Voss; Stefan C. Teilmann; Ian H. Lambert

doi:10.1016/j.cellbi.2005.02.003

High expression of the taurine transporter TauT in primary cilia of NIH3T3 fibroblasts

Søren Tvorup Christensen, Jesper W. Voss, Stefan C. Teilmann, Ian H. Lambert

Cell Biology and Physiology

17 Citations (Scopus)

Abstract

Taurine, present in high concentrations in various mammalian cells, is essential for regulation of cell volume, cellular oxidative status as well as the cellular Ca²⁺ homeostasis. Cellular taurine content is a balance between active uptake through the saturable, Na⁺-dependent taurine transporter TauT, and passive release via a volume-sensitive leak pathway. Here we demonstrate that: (i) TauT localizes to the primary cilium of growth-arrested NIH3T3 fibroblasts, (ii) long-term exposure to TNFa or hypertonic sucrose medium, i.e., growth medium supplemented with 100 mM sucrose, increases ciliary TauT expression and (iii) long-term exposure to hypertonic taurine medium, i.e., growth medium supplemented with 100 mM taurine, reduces ciliary TauT expression. These results point to an important role of taurine in the regulation of physiological processes located to the primary cilium.

Original language	English
Journal	Cell Biology International
Volume	29
Issue number	5
Pages (from-to)	347-351
ISSN	1065-6995
DOIs	https://doi.org/10.1016/j.cellbi.2005.02.003
Publication status	Published - 2005

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@article{02d5cf406c3711dcbee902004c4f4f50,

title = "High expression of the taurine transporter TauT in primary cilia of NIH3T3 fibroblasts",

abstract = "Taurine, present in high concentrations in various mammalian cells, is essential for regulation of cell volume, cellular oxidative status as well as the cellular Ca2+ homeostasis. Cellular taurine content is a balance between active uptake through the saturable, Na+-dependent taurine transporter TauT, and passive release via a volume-sensitive leak pathway. Here we demonstrate that: (i) TauT localizes to the primary cilium of growth-arrested NIH3T3 fibroblasts, (ii) long-term exposure to TNFa or hypertonic sucrose medium, i.e., growth medium supplemented with 100 mM sucrose, increases ciliary TauT expression and (iii) long-term exposure to hypertonic taurine medium, i.e., growth medium supplemented with 100 mM taurine, reduces ciliary TauT expression. These results point to an important role of taurine in the regulation of physiological processes located to the primary cilium.",

author = "Christensen, {S{\o}ren Tvorup} and Voss, {Jesper W.} and Teilmann, {Stefan C.} and Lambert, {Ian H.}",

note = "Keywords: Taurine transporter; NIH3T3 cells; Primary cilia; Ca2+ homeostasis; Ion channels; Tumour necrosis factor a",

year = "2005",

doi = "10.1016/j.cellbi.2005.02.003",

language = "English",

volume = "29",

pages = "347--351",

journal = "Cell Biology International",

issn = "1065-6995",

publisher = "Academic Press",

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TY - JOUR

T1 - High expression of the taurine transporter TauT in primary cilia of NIH3T3 fibroblasts

AU - Christensen, Søren Tvorup

AU - Voss, Jesper W.

AU - Teilmann, Stefan C.

AU - Lambert, Ian H.

N1 - Keywords: Taurine transporter; NIH3T3 cells; Primary cilia; Ca2+ homeostasis; Ion channels; Tumour necrosis factor a

PY - 2005

Y1 - 2005

N2 - Taurine, present in high concentrations in various mammalian cells, is essential for regulation of cell volume, cellular oxidative status as well as the cellular Ca2+ homeostasis. Cellular taurine content is a balance between active uptake through the saturable, Na+-dependent taurine transporter TauT, and passive release via a volume-sensitive leak pathway. Here we demonstrate that: (i) TauT localizes to the primary cilium of growth-arrested NIH3T3 fibroblasts, (ii) long-term exposure to TNFa or hypertonic sucrose medium, i.e., growth medium supplemented with 100 mM sucrose, increases ciliary TauT expression and (iii) long-term exposure to hypertonic taurine medium, i.e., growth medium supplemented with 100 mM taurine, reduces ciliary TauT expression. These results point to an important role of taurine in the regulation of physiological processes located to the primary cilium.

AB - Taurine, present in high concentrations in various mammalian cells, is essential for regulation of cell volume, cellular oxidative status as well as the cellular Ca2+ homeostasis. Cellular taurine content is a balance between active uptake through the saturable, Na+-dependent taurine transporter TauT, and passive release via a volume-sensitive leak pathway. Here we demonstrate that: (i) TauT localizes to the primary cilium of growth-arrested NIH3T3 fibroblasts, (ii) long-term exposure to TNFa or hypertonic sucrose medium, i.e., growth medium supplemented with 100 mM sucrose, increases ciliary TauT expression and (iii) long-term exposure to hypertonic taurine medium, i.e., growth medium supplemented with 100 mM taurine, reduces ciliary TauT expression. These results point to an important role of taurine in the regulation of physiological processes located to the primary cilium.

U2 - 10.1016/j.cellbi.2005.02.003

DO - 10.1016/j.cellbi.2005.02.003

M3 - Journal article

SN - 1065-6995

VL - 29

SP - 347

EP - 351

JO - Cell Biology International

JF - Cell Biology International

IS - 5

ER -

High expression of the taurine transporter TauT in primary cilia of NIH3T3 fibroblasts

Abstract

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